CAZyme Information: MGYG000001136_00156

Basic Information

• Species: CAG-103 sp900543625
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; CAG-103; CAG-103 sp900543625
• CAZyme ID: MGYG000001136_00156
• CAZy Family: GT84
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2377	MGYG000001136_3|CGC1	257433.11	8.3307

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001136	2106281	MAG	China	Asia

• Gene Location: Start: 26440; End: 33573 Strand: -

Full Sequence      

MAETNAGALREAGARDARALTVTGRRRARTGSIRRAIKALTRTAKAVTPETPNGAWLRDN
RTLARTAADDAAAALRHAHTLRASGAQTVLHACCAGLLRACGNALTPKAAQAYLAGFQDV
LPLETAELALLVPGLQAAAVCALADTYAHDGAAAPALFTSLRALGTAAWAALTERCDRVG
RILARDPAGVYPAMDAATRTRYRQTVARLARRTGRTEIDIAEDVLARAQSGAGARRHVGW
FLLREVLGAPEKRRDGAWYIAAVIVGTLALSLLLGFATGSVSGAVLLLIPVSEAVKGLLD
AVLLRVTKPRFVPRLALRRGIPPEGKTLCVIAALLTCPGDAHTLAARLEEYALCNRDAGE
QLLFGLLADLPEADMEHAPIDPAILRAARTEIETLNARYGGRFYLFTRERVQTPDGKWSA
WERKRGALLRLAQLLCGEADAMHCAAGDAAALRGTAYLLTLDADTRLTPGAARALVGAML
HPLNRAVVDARRGVVTHGYGLLHPRMATELQSARASDWARVFAGPGGSDPYGGVCGELYM
DCFDRGGFSGKGILDVRALLTCCGGDALPEQRILSHDALEGAYLHGGFLGDVELTDTFPA
APLAWGARAHRWIRGDWQNAPWIFSRRARALHPIDRFRLADSLRRSLVAPATWAAVFLGC
VLRWPGLRLAAYAALLALAQGLIFALGHPIVHPADARVRYHSSVLPGLAAAVVQTVLRLI
LLPWETVVNASAIVTALWRMTVSHRDLLQWQTAAQRAAKRDGLGAYLRALWPASALGLLT
AVLTPSPTGLAAGIVWTLSPFVLAALGAPNAPGAAPLTHAAQRELLGWAKATWHYFETFC
TAGEHYLPPDNVQTQPPTGTAHRTSPTNMGFALLSALCAHALGIDNGRGLALAERMLTTM
EQLPRWNGHFYNWYHTCTLRPMPPLYVSTVDSGNCAAALLAAANALRGWGQGALAVRAQA
LCDGMDFALLYDPQRRLMHIGIDTGSGKRSEGYYDLLESEARLTSYFAVARGDVPREHWR
ALSRAQVQHERYRGCVSWSGSVFEYLMPELFLPPQRDSLLWESAKFCLRVQRRRVRAGQP
WGVSESAYFALDSALSYRYKAHGCAALAMQPDMDRELVLSPYSAFLALAVAPRAAVRDLR
RFAARGLLGQYGFREALDCTRARTGGGGQIVHCVMAHHQGMSLLSACNALCGGQVQRWFL
ADPAMRAHMSLLSERLPLGAPALRRRPETQRGKPHLRSLPDYTVEGTGVDAEAPRCTLLS
NGTYCLAVTEAGQSFARWHALSVCRRGTRSDPAAAPMLSLETGGACTPLLPGTADAARWR
FTVRAAQFSTRHGSVTAVQTFTVPERVNGEVRALTLSSPDGIDGTVRLTFEAVLAPWEDY
VNHPAFARLGFQSVLRRNALLLRRLGRGEKPETWLCAACDRQADFCAEGAIPGWLRAGYV
QVRVAVHTPPDTPWRVRFALGVGAHEDDAFAAAQRALVLPPESAAVLPGALAAQYGMTDA
ELEGAMAHISPLLFPAVSPDAAAEQLKRPALWAHGISGDLPVWCARPEAHVLRQWALLRA
LGIGCDLALCTSDGADYLQPTRAKVRGALAALDLAHCLDAPGGVHLVPEDAFADVAAAAA
IRPGMAHTRCTLPLLPPPGDRRTMPTGSDVHWDADGTVAFTGLPPRAWSNVLTNGRFGFL
ATDAGTGHLWYRNAHTGRINRWLCDPWVLRGTETLRMTTRAGTVSLFDDDGHARVEYGFG
WAAWERTVDGMSVRVTAFVPEDANARVLLIECAGRARITWHTDLVCAGRDADAPAVVTAY
SGGVFTAENARADTPLRVCAAVGMPLTGWTCDRFSFLRGQMDRRAGAGLSPCFALEGVVE
RQGVLVCGCDTRANLLRLTQPDEAVHALHRVRERWLGAVSRLWMTTPDENMNRYLGGWAA
YQTLCCRLLARTSLYQNGGAFGFRDQLQDAVNLLLLDSKPAREQIGCACAHQFSAGDVCH
WWHTGFGVEHGVRTRISDDLLWLPWAVCEYAQKTGDTQILSAEYPFLAGEELEENERDRY
QPLTPGAETGTVLEHCRRAFMRVLARGVGAHGLLRIGTGDWNDAFDRVGAQGRGESVWLT
WFFAVTARKFAALIGGHAAEQLTLAADRCTRAAEAAWDGAWYRRGYYDDGQPLGSEKSGE
CRIDAIAQAFAAFDTHADPTHVHAALTSAVTQLFDREHNVVRLFDPPITRRTPETGYVRS
YGAGLRENGGQYTHGALWLAMALLRTGRTDEGAEILRAVLPAAHDPARYEGEPYVLAADV
AAGDNAGVAGWTWYTGAAGWYLRIAAEELLGLHLRGGVLYPEPRLPAGWPECAVRWRDGA
GLLHTIRLRPDGVTVDEKPYDGGGIGKKRPKPYSQDR

Enzyme Prediction     

EC	2.4.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH94	1525	2357	1.9e-174	0.8494208494208494
GT84	993	1203	2.2e-69	0.9906976744186047

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3459	COG3459	9.00e-131	1665	2353	293	1023	Cellobiose phosphorylase [Carbohydrate transport and metabolism].
pfam17167	Glyco_hydro_36	9.26e-79	1895	2312	2	424	Glycosyl hydrolase 36 superfamily, catalytic domain. This is the catalytic region of the superfamily of enzymes referred to as GH36. UniProtKB:Q76IQ9 is a chitobiose phosphorylase that catalyzes the reversible phosphorolysis of chitobiose into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. The full-length enzyme comprises a beta sandwich domain and an (alpha/alpha)(6) barrel domain. The alpha-helical barrel component of the domain, this family, is the catalytic region.
cd11756	GH94N_ChvB_NdvB_1_like	3.21e-23	1664	1860	28	254	First GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This first of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.
pfam06165	Glyco_transf_36	2.41e-22	1665	1860	1	233	Glycosyltransferase family 36. The glycosyltransferase family 36 includes cellobiose phosphorylase (EC:2.4.1.20), cellodextrin phosphorylase (EC:2.4.1.49), chitobiose phosphorylase (EC:2.4.1.-). Many members of this family contain two copies of this domain.
cd11753	GH94N_ChvB_NdvB_2_like	2.36e-19	1196	1389	2	205	Second GH94N domain of cyclic beta 1-2 glucan synthetase and similar domains. The glycoside hydrolase family 94 (previously known as glycosyltransferase family 36) includes cyclic beta 1-2 glucan synthetase (EC:2.4.1.20) or ChvB (encoded by the chromosomal chvB virulence gene). This second of two tandemly repeated GH94-N-terminal-like domains has not been characterized functionally. Some beta 1-2 glucan synthetases are annotated as NdvB (nodule development B) gene products, glycosyltransferases required for the synthesis of cyclic beta-(1,2)-glucans, which play a role in interactions between bacteria and plants.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QBB66089.1	0.0	13	2363	18	2383
ALP93881.1	0.0	13	2081	18	2102
AVQ95160.1	0.0	89	2365	106	2528
AYF37850.1	0.0	89	2365	106	2528
QCN91406.1	0.0	89	2365	106	2528

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2CQS_A	9.88e-36	1644	2362	15	824	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Ammonium Sulfate [Cellulomonas gilvus],2CQT_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],2CQT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Crystallized from Sodium/Potassium Phosphate [Cellulomonas gilvus],3QFY_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFY_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and Isofagomine [Cellulomonas gilvus],3QFZ_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QFZ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Sulfate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_A Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus],3QG0_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Complexed with Phosphate and 1-Deoxynojirimycin [Cellulomonas gilvus]
3AFJ_A	3.96e-35	1644	2362	15	824	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127],3AFJ_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase triple mutant [Cellulomonas gilvus ATCC 13127]
3ACT_A	6.91e-35	1644	2362	15	824	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant [Cellulomonas gilvus ATCC 13127],3ACT_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase Histidine mutant [Cellulomonas gilvus ATCC 13127]
3ACS_A	1.59e-34	1644	2362	15	824	CrystalStructure of Cellvibrio gilvus Cellobiose Phosphorylase W488F mutant [Cellulomonas gilvus ATCC 13127],3ACS_B Crystal Structure of Cellvibrio gilvus Cellobiose Phosphorylase W488F mutant [Cellulomonas gilvus ATCC 13127]
3S4C_A	2.17e-32	1651	2362	5	804	Lactosephosphorylase in complex with sulfate [Cellulomonas uda],3S4D_A Lactose phosphorylase in a ternary complex with cellobiose and sulfate [Cellulomonas uda]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P20471	2.02e-284	157	2351	288	2808	Cyclic beta-(1,2)-glucan synthase NdvB OS=Rhizobium meliloti (strain 1021) OX=266834 GN=ndvB PE=1 SV=2
B9K7M6	5.39e-37	1877	2365	291	782	Cellobiose phosphorylase OS=Thermotoga neapolitana (strain ATCC 49049 / DSM 4359 / NBRC 107923 / NS-E) OX=309803 GN=cbpA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000052	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
256	278