CAZyme Information: MGYG000001157_00826

Basic Information

• Species: Gemmiger sp900540775
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Gemmiger; Gemmiger sp900540775
• CAZyme ID: MGYG000001157_00826
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
696		79104	7.282

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001157	2792015	MAG	Austria	Europe

• Gene Location: Start: 6437; End: 8527 Strand: +

Full Sequence      

MKQRKETPNSSDLPIYLFKQGNNQEAYRYFGAHLCEQNGEAGAVFRVWAPHAKAVSIVGD
FNSWTPGEHPMEKVTDGIWERFVPGIKQFDVYKYCITTPADELVYKADPYAFHTETRPSN
GSKVYDIEGYTWGDAAWVKDEDKRDVINSPMSIYELQAGSWKMKDPDNGVPYNYAELADE
LIPYIKDMGYTHVELLPITEYPFDGSWGYQVTGYFAPTSRYGTPKDFMTFVDKLHQAGIG
VILDWVPAHFPKDGYGLYMFDGAPCYEDPNPRRGEHKEWGTMVFNYGMKEVESFLISSAM
FWVDHYHIDGLRVDAVASMLYLDYNRKDGEWEQNVKGGKENLEAIAFLQKLNTAVLGRYP
HKMMIAEESTAWPLVTKPASDGGLGFNFKWNMGWMNDMLSYMKTDPLFRAGNHGKVTFSF
FYAFSENFVLPISHDEVVHGKCSLLNKMPGDYEEKFANLRTFFGYMMAHPGKKLLFMGQD
FGQFIEWDEKKQLDWMLLSYDKHRELQGYVKDLNHFYRDTPALWQVDYSWEGFQWIVPDD
SKQSVIAFLRRDAAGKMILVVCNFNPVLRKDYQMGVPNPGSYKEILNSDDKKYGGTGVTN
GTVKAKKGPMHGFDQHISLTLPPLSTIYLSVPAARKPKAEKPTTEKAADKPAKTAKKPVA
KKAAKPTAKADAPAAEAKTQAAPKRRGRPPKAKTTV

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	183	483	3.2e-158	0.9966777408637874
CBM48	30	114	1.5e-19	0.8947368421052632

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	4	629	1	625	1,4-alpha-glucan branching protein GlgB.
COG0296	GlgB	0.0	12	631	8	628	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK14705	PRK14705	0.0	8	634	601	1224	glycogen branching enzyme; Provisional
PRK14706	PRK14706	0.0	30	646	27	636	glycogen branching enzyme; Provisional
PRK05402	PRK05402	0.0	8	634	98	726	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AXB29737.1	0.0	3	669	5	668
CBL01646.1	0.0	3	653	5	651
AXA81819.1	0.0	3	650	5	649
ATL90043.1	0.0	3	655	5	648
CBK98932.1	0.0	3	652	5	651

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQZ_A	2.37e-235	7	637	126	779	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQW_A	2.37e-235	7	637	126	779	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	3.36e-235	7	637	126	779	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	6.74e-235	7	637	126	779	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	1.35e-234	7	637	126	779	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B8CVY1	3.10e-258	11	628	7	624	1,4-alpha-glucan branching enzyme GlgB OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=glgB PE=3 SV=1
Q1AZ86	1.03e-254	8	629	97	717	1,4-alpha-glucan branching enzyme GlgB OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129 / PRD-1) OX=266117 GN=glgB PE=3 SV=1
O66936	1.92e-249	11	629	8	626	1,4-alpha-glucan branching enzyme GlgB OS=Aquifex aeolicus (strain VF5) OX=224324 GN=glgB PE=3 SV=1
Q8DLB8	2.12e-247	7	631	103	750	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
P16954	3.64e-245	7	631	112	759	1,4-alpha-glucan branching enzyme GlgB OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=glgB PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001157_00826.