CAZyme Information: MGYG000001160_01512

Basic Information

• Species: CAG-274 sp000432155
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; CAG-274; CAG-274; CAG-274 sp000432155
• CAZyme ID: MGYG000001160_01512
• CAZy Family: CBM51
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1503	MGYG000001160_21|CGC3	167613.03	4.3354

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001160	2196853	MAG	Austria	Europe

• Gene Location: Start: 68119; End: 72630 Strand: +

Full Sequence      

MNLKKTIALGLSTLMTLQITPLNIFADETSQELTVSSISASDPKMSSDYWQNHRVSTNSD
DYTYISAYSNNAGQYSTSYLKYAFDGNWSTHWETGNGYGTVTNYVDVTFNKTTKIDRILY
ATRQDGAKGKGYPTTATIYSYNSETDTWDKVAVGSSSVSNSYVLITLPQATDFEKLRFEF
TVANNGWASASEFVFLRPDDTVLDGNVTISGTSAIGKTLTANDNLTVGNSKNLGYQWQYS
DDGTVWTDIENATEKNYTITEQKAYYRVKIYDKTNEYYGNLYSEAYKGSMVAKITGSLKV
GCVVTAETEYVNPEDTLYYKWQYSADGTTFTDIPSATSLKYTVPASMSNKYIRLGISGNN
KDYIYSDNSFVSVSAVLSGPCQVNSEILAQLKGAENTEYTYQWQICDTADGEFTDISSAT
SAKYTPTESQLNKYIRVLLTITETGELLYSQPRLVSSSGTYPDRTGEYMYISDVPSSALI
SSSVGYSTLKYDTNVEDGIIALKVNGQKKQFLKGLGAHAPATLVYDISYLVDDYGYDTFT
GYLGVDYSKGSNGNGVIFNIYTSQDNSTWTQVKNTGVLKGDSECQYVTIDLKNAKYLKFD
ISSNGDKGYDHSVIANGKFISSDYKEPSADNFKFIKTVSQYESELAGLSDYESETYRKIL
YQKNFVERTGYDFLITLASENENNREALEWFLDDFEALNYYANAGNTILGSYEKAAMVLT
QLYKNYSLDMSNSLYKRMFVALMFSHAGSIYFWADSTKTSNPVRRYAIYKKLYENGCLVN
SVFENLTVPEMRWVMNSISDDNQIEWLNYYARKKSAAKAGVAFSDLADNQLILNSYSYMG
YITGYNYYLDKYYTDENKASWTEKYHLVNTDDDTNDDIYDINIPFESGHPKLWIVFEEGA
VCGGISKVGVNLLTAFGIPSVVIGQPGHAAYLKYELDDPSKGENALAKWSIWNDVYGWTK
SERGDTLLLGWGAQSWAKGYRVSYVPLAQAALNDYDNFQKAEDLVKIAQMSNTDKAIELY
RQAIEIQNFNLDAWDGLITAYKSAEKTSDDYMELAKEIISALTYFPQPMNDMLQNAIKPE
ISSEADIADFNIMTLNALKSASVATSEQSLQPDICKTMADYILGNENLAIATFSFDGENA
NTIVLSDQFTDGGTEFLYSIDGGENWVNAGTNNSHKLTDEEISKITADNDLLVKLQGASS
YYTIDIKAGSAPSGLYNNDNENKVIGATSKYQWSEDRKTWTNFTDDTVFEGDRTVSVRIG
ANGTTLAGSSSQYTFTTDTYTADRSYISISNVKVLAYSSAQSDNESASKSIDGNINTIWH
TTYTTNSDLNRFIAYEFNKPVLLTSIDYTPRQTGNFNGVFTKCSVYTSKDGTNWTKAGTA
TWASDRTKKTVNLDTPVYTKYVKVVGDEAGANFGSAAMIEFYERLNSDNYDINKDNSVDN
KDVALLLKYVMGVNLSSDISFENADFNGDGNIDMLDVITLKNYTDNNTVETTTETTETTT
EQQ

Enzyme Prediction     

No EC number prediction in MGYG000001160_01512.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CBM51	470	619	4.7e-21	0.9850746268656716
CBM32	1298	1420	5.2e-17	0.9032258064516129

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam08305	NPCBM	4.86e-29	468	620	2	136	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
smart00776	NPCBM	1.21e-17	470	619	6	144	This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins.
pfam00754	F5_F8_type_C	1.41e-14	1298	1421	5	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd14256	Dockerin_I	2.18e-12	1431	1483	3	55	Type I dockerin repeat domain. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. The cellulosome consists of scaffoldin, a noncatalytic scaffolding polypeptide, that comprises repeating cohesion modules and a single carbohydrate-binding module (CBM). Specific calcium-dependent interactions between cohesins and dockerins appear to be essential for cellulosome assembly. This subfamily represents type I dockerins, which are responsible for anchoring a variety of enzymatic domains to the complex.
pfam00404	Dockerin_1	2.66e-11	1431	1483	2	54	Dockerin type I repeat. The dockerin repeat is the binding partner of the cohesin domain pfam00963. The cohesin-dockerin interaction is the crucial interaction for complex formation in the cellulosome. The dockerin repeats, each bearing homology to the EF-hand calcium-binding loop bind calcium.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AMN35280.1	6.46e-194	458	1437	38	994
AQW23377.1	3.68e-192	433	1437	5	994
ATD49073.1	4.25e-192	433	1437	10	999
SQF04750.1	1.48e-144	460	1427	134	1102
QTZ58125.1	1.97e-144	467	1427	139	1100

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7JND_A	2.89e-22	27	199	39	208	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124],7JNF_A Chain A, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]
7JNB_A	5.16e-22	27	199	39	208	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]
7JFS_A	2.35e-21	65	199	54	190	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]
2V72_A	3.79e-14	1293	1423	15	143	Thestructure of the family 32 CBM from C. perfringens NanJ in complex with galactose [Clostridium perfringens]
7JS4_A	6.50e-12	446	618	586	751	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P0DTR4	2.45e-09	1307	1422	530	644	A type blood N-acetyl-alpha-D-galactosamine deacetylase OS=Flavonifractor plautii OX=292800 PE=1 SV=1
I1S2N3	2.98e-09	1299	1423	61	188	Galactose oxidase OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=GAOA PE=3 SV=1
P0CS93	2.98e-09	1299	1423	61	188	Galactose oxidase OS=Gibberella zeae OX=5518 GN=GAOA PE=1 SV=1
P29767	9.36e-06	1305	1423	67	185	Sialidase OS=Clostridium septicum OX=1504 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000260	0.999014	0.000201	0.000184	0.000165	0.000148

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001160_01512.