CAZyme Information: MGYG000001164_00011

Basic Information

• Species: Prevotella lascolaii
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella lascolaii
• CAZyme ID: MGYG000001164_00011
• CAZy Family: GH76
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1320	MGYG000001164_2|CGC1	145623.6	4.4964

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001164	3302780	MAG	Austria	Europe

• Gene Location: Start: 10063; End: 14025 Strand: -

Full Sequence      

MKKLLLTVLMSGITGMASADIVPGKTYRIASAANEERVMFVDNSSLANNTSVVVWTDTEV
PAQQWEAIANGDGTFSFMNVFTGKYLARNSALISASSKVLQTATASNMGQWSVDAVSGHD
GYYVITQSSASSTFILQTAATSDGQTMTYQAWSDGGTPTKEQMWKFEEVEPISSFTDTQR
TQMMDAWLAQHLRDRGSNGKTFGNGGGWGDAEMLETILDAYETTGREEYLDVFRSVFNYF
KWAVGTDWLKLKYDDAYKWYGHDFNDDVMWMIIASARAYHLTGNTMYRSYAKSNFDRIYD
RAYNQWGMMRWAEQSGNKNGTNSCINGPTEVAACYIAMAQTDENEREQYYEVARSLYEKQ
RLYLFNQQTGEVYDSFTWDESTNLPGGYNYWVSTYNQGTMLGAALMLYKHYGDEMYKRDA
EKIVERTKESLCNSDGIINVCQTVDGDLCGFKGVLMRYLRRYVVEMRKTEDVEWMKDNAF
MAFNNRNSYGITSSAWLTKSNENWKSDTEKDGNGNFKSFQNQPFGNSTAISAAFNAPLST
SFIGRDAYSTIEAENFNTLRGVYVTTGTGGNAAEITNIRNGFYTCYANVDFGTRPARTVE
IRVSDSSTANAKIEIRLDRADGTLIGTADIPQGGGWKTVTADIAPTDGTHDIYLVYQPGS
SSAGCFNVDWLKFTTDAMELPGDITKNGGVLTSSVDGTQTGALTDNSLTSAVSVQATAAQ
FTYRSPVAVTLKGYSIGSSAADVSTDARSWTLSASNDGSTWTVLDEQTDQTFATRCTLRR
YDAAADKAYTMFRLDVTKNNGGSALAVSEWQLHGLAISATDITADGGALNEGDKSATDKN
ASTTAVIDGETNSIFRYNSKALYRLTSYSITSSAAGNAPKSWTLYGYENAEWIPIDSRSN
QQTAYNGCTQFYKVTTDKTYQNFRLVINDTEARAELAELQLFGDIAPNGELYNDITDNGG
TLLASDNAGETALRPLIDNDANTTYTLPFDGEAWVEYRSEIPVRITTLRVCAGYDTDRSP
KNYTLQASDDGSSWTTLANVSNARFNTKGSFKNTSVSNSNTYKRFRITFTQASDNAADAI
VMGDIQIHGICLSDNDIFSKGGVMTSQISNDNADENLDKLYDGTADTKFVFDYLGNPWIE
FQADEPAAANLYSITSANDEASRDPMGWELQGSDNGRRWTTIDTRTGQSFYARKNTQFYS
FDNDHSYRFYRLVLTENGGARLAQMSEWQLFYSEKIATGIDDITTGEIRLNVYPNPVSDV
LHIDMPADGTVTIYNTGGQAVATLSLNAGVNSIPFAQYGAGMYIVKAKAGNSTLTAKVVK

Enzyme Prediction     

No EC number prediction in MGYG000001164_00011.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH76	176	505	8.1e-48	0.88268156424581
CBM6	551	674	3.3e-27	0.8695652173913043

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd04084	CBM6_xylanase-like	1.66e-31	549	673	1	123	Carbohydrate Binding Module 6 (CBM6); many are appended to glycoside hydrolase (GH) family 11 and GH43 xylanase domains. This family includes carbohydrate binding module 6 (CBM6) domains that are appended mainly to glycoside hydrolase (GH) family domains, including GH3, GH11, and GH43 domains. These CBM6s are non-catalytic carbohydrate binding domains that facilitate the strong binding of the GH catalytic modules with their dedicated, insoluble substrates. Examples of proteins having CMB6s belonging to this family are Microbispora bispora GghA, a 1,4-beta-D-glucan glucohydrolase (GH3); Clostridium thermocellum xylanase U (GH11), and Penicillium purpurogenum ABF3, a bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase (GH43). GH3 comprises enzymes with activities including beta-glucosidase (hydrolyzes beta-galactosidase) and beta-xylosidase (hydrolyzes 1,4-beta-D-xylosidase). GH11 family comprises enzymes with xylanase (endo-1,4-beta-xylanase) activity which catalyze the hydrolysis of beta-1,4 bonds of xylan, the major component of hemicelluloses, to generate xylooligosaccharides and xylose. GH43 includes beta-xylosidases and beta-xylanases, using aryl-glycosides as substrates. CBM6 is an unusual CBM as it represents a chimera of two distinct binding sites with different modes of binding: binding site I within the loop regions and binding site II on the concave face of the beta-sandwich fold.
pfam03663	Glyco_hydro_76	3.34e-27	208	482	36	313	Glycosyl hydrolase family 76. Family of alpha-1,6-mannanases.
smart00606	CBD_IV	3.49e-27	544	673	1	129	Cellulose Binding Domain Type IV.
pfam03422	CBM_6	7.24e-24	552	674	1	124	Carbohydrate binding module (family 6).
pfam18962	Por_Secre_tail	7.78e-11	1252	1319	1	71	Secretion system C-terminal sorting domain. Species that include Porphyromonas gingivalis, Fibrobacter succinogenes, Flavobacterium johnsoniae, Cytophaga hutchinsonii, Gramella forsetii, Prevotella intermedia, and Salinibacter ruber have on average twenty or more copies of this C-terminal domain, associated with sorting to the outer membrane and covalent modification. This domain targets proteins to type IX secretion systems and is secreted then cleaved off by a C-terminal signal peptidease. Based on similarity to other families it is likely that this domain adopts an immunoglobulin like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT74831.1	4.10e-260	1	1320	1	1310
QIA07414.1	1.48e-88	17	657	60	658
QEC70572.1	1.44e-62	18	535	25	494
QOV19890.1	6.01e-62	205	676	56	495
AYD48939.1	3.34e-61	20	498	27	472

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5M77_A	5.05e-47	208	675	73	524	ChainA, Alpha-1,6-mannanase [Niallia circulans],5M77_B Chain B, Alpha-1,6-mannanase [Niallia circulans]
4BOJ_A	3.61e-31	153	506	8	334	ChainA, Alpha-1,6-mannanase [Niallia circulans],4BOJ_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4BOJ_C Chain C, Alpha-1,6-mannanase [Niallia circulans]
4BOK_A	6.21e-31	208	506	39	331	ChainA, Alpha-1,6-mannanase [Niallia circulans]
4D4A_A	3.16e-30	208	506	60	352	ChainA, Alpha-1,6-mannanase [Niallia circulans],4D4A_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4B_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4B_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4C_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4C_B Chain B, Alpha-1,6-mannanase [Niallia circulans],4D4D_A Chain A, Alpha-1,6-mannanase [Niallia circulans],4D4D_B Chain B, Alpha-1,6-mannanase [Niallia circulans],5N0F_A Chain A, Alpha-1,6-mannanase [Niallia circulans],5N0F_B Chain B, Alpha-1,6-mannanase [Niallia circulans],6ZBX_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBX_B Chain B, Alpha-1,6-mannanase [Niallia circulans],7NL5_A Chain A, Alpha-1,6-mannanase [Niallia circulans]
5AGD_A	1.42e-29	208	506	60	352	ChainA, Alpha-1,6-mannanase [Niallia circulans],5AGD_B Chain B, Alpha-1,6-mannanase [Niallia circulans],6ZBM_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBW_A Chain A, Alpha-1,6-mannanase [Niallia circulans],6ZBW_B Chain B, Alpha-1,6-mannanase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q45071	7.42e-15	540	674	373	511	Arabinoxylan arabinofuranohydrolase OS=Bacillus subtilis (strain 168) OX=224308 GN=xynD PE=1 SV=2
P10478	9.61e-10	535	690	286	437	Endo-1,4-beta-xylanase Z OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=xynZ PE=1 SV=3

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000360	0.998886	0.000216	0.000204	0.000164	0.000143

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001164_00011.