CAZyme Information: MGYG000001205_00054

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Gemmiger
• CAZyme ID: MGYG000001205_00054
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1148	MGYG000001205_1|CGC1	126313.78	5.4669

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001205	2359919	MAG	Austria	Europe

• Gene Location: Start: 60186; End: 63632 Strand: -

Full Sequence      

MIRTDFSGGWLFAAAAPGGPQPPQAEFIPVDLPHDWQIRHADDLYRDGTLYYRKQFACTP
AAGERVALWFGGAYMDTAVFINGREAGQWKNGYTSFWFDITGLLQPGENEVLVRCELRHP
NSRWYSGAGLFRTVQLWRMPAAHLMPDGFYVAAREGQGNVWTVRCTAEIGLCAGAALPEA
LDLALCGPEGELAACRVPAAVWKPLAARPWRTGQEQAIYQAEAEFTLPSPRLWDLGEPNL
YRVEAALGGPAPDTLETKFGCRTIELSPDKGLLLNHRHVYLRGVCLHHDFGCLGAAFEPD
AAARQLRLLQQMGANSIRTGHVVPDPQLMDLADEMGFLINDEIFDCWRSAKNPYDYARFF
DAWQERDVAAWVRRDRNHPSLLFWSIGNEIYDTHAGPEGADTMRLLMAEVAEHDPERNGV
PTFGSNYLPWPNTQACADIIKVAGYNYGERMYAQHHAEHPDWIIYGSETASVVMSRGVYH
FPLSQPLLSDDDEQCSSLGNSRTSWGAESLDICLTTERHHPYTLGQYLWTGWDYIGEPTP
YHTRNSYFGMVDTAGFPKDAFYVVQAAWLDPAVQPMVHLFPYWDWNPGQTVDVCACTNAA
SVELFLDGESLGRRAPDPDKGRTVSWQVAYRPGTLRAVAYDEQGAAVAQDERRSFGDSAA
LCVKADRTALSGDGRELAFLTITAADAGGNPVANANDRVTVRVRGAGVLVGLDNGDSTDP
DEYQTTSRRLFSGMLLAVVAGNGSAGEITVEVSAPGLRSAVLALTARPFAGPARHRLPPL
PPAQDPVCVPVRKLELTAGHTRLTPFHSKTTVRAVRLPAAAGAGEAIEWRLTDDRGDPAA
NAVLHADGDSATVEAKGDGALRVRAMLRNGRRTPQFISQLDLQVEGLGQLYTNPYDFVPA
GRYEASYGDIGNGNERGISTSRTGRSWVLYRNIDFGPDGSDTVELPIFSLDGEPTAIRFW
DGEPYVIGSTMIGERIYHKPKMWNVYQPDTFRLDKRLTGVGSFAVELNTKVHIKGFQFTP
HSRAFDTLAAGQCDAVNGDSFTRAGTQVLGIGNNVSLTFRRMDFGKNGCSGVRLRGRSAL
PANTVHLLFDSEGPGGSERRIVEFGPQPDWGEQTFHFAPVKGKRQVTFLFLPGTQFDFDS
FTFLGDPT

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	4	567	6.1e-99	0.6050531914893617

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	7.43e-61	3	721	12	770	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	7.22e-36	663	762	3	102	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	3.60e-33	3	455	12	479	beta-D-glucuronidase; Provisional
PRK10340	ebgA	5.42e-26	28	389	74	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam16355	DUF4982	4.77e-21	588	650	1	62	Domain of unknown function (DUF4982). This family is found in the C-terminal of uncharacterized proteins and beta-galactosidases around 680 residues in length from various Bacteroides species. The function of this protein is unknown.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCG34875.1	0.0	1	1144	1	1148
AGA58497.1	0.0	3	1143	4	1139
AET60078.1	0.0	3	1143	6	1139
QOT12659.1	0.0	3	1144	4	1150
AIQ48506.1	0.0	7	1144	8	1172

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7CWD_A	1.42e-159	1	775	1	810	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	5.26e-152	3	759	9	800	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
4CU6_A	1.94e-130	3	759	15	837	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU7_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4],4CU8_A Unravelling the multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA [Streptococcus pneumoniae TIGR4]
4CUC_A	5.26e-130	3	759	15	837	Unravellingthe multiple functions of the architecturally intricate Streptococcus pneumoniae beta-galactosidase, BgaA. [Streptococcus pneumoniae TIGR4]
6QUB_B	6.45e-130	4	768	10	857	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	2.91e-140	3	766	44	848	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	1.14e-72	2	733	47	787	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.68e-43	25	639	77	702	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	5.36e-29	27	769	64	798	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
Q59140	1.12e-22	56	389	129	434	Beta-galactosidase OS=Arthrobacter sp. (strain B7) OX=86041 GN=lacZ PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.995724	0.004262	0.000035	0.000006	0.000003	0.000006

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001205_00054.