CAZyme Information: MGYG000001209_01291

Basic Information

• Species: Porphyromonas uenonis_A
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Porphyromonadaceae; Porphyromonas; Porphyromonas uenonis_A
• CAZyme ID: MGYG000001209_01291
• CAZy Family: GT3
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
542	MGYG000001209_22|CGC1	60591.89	5.6231

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001209	2042909	MAG	Austria	Europe

• Gene Location: Start: 16330; End: 17958 Strand: +

Full Sequence      

MSDRYFISEVSWEVANKVGGIYTVLSSRAGEMMLRHGAEQVLFVGPHLRPMEEMVDFRAE
TFCITLPSHISLLGVELPITQGYWLTPGGEAQTILVDYTPLASVRDQLYYLMWQHYGIQS
DLGYGDYDDSCLFAVAAAATLLAVTPQLALPGSQPIAIYNEWITGMGLLYHRLQQPNLHS
LFITHATTVGRSICSNGKDLYRYFAGYHGDQMASELHVEAKHALEKAAAHTATFFATVSE
VTALECTQLLERTPVVLPNGFHALATSRFKTKARQRLIDVASQLYGKTFDPKGTTLVATS
GRYEYRNKGIDLIVNSLESLLQQQAKLSSDLILYFFIPAWVAEPRADLAYLLSEPNPESS
TTPLEYPYLTHWLHNLREDTLAGHLGELLRRGATAQHIYPIFVPTYLDGHDGILGLPYYD
LLSALDLTLFPSYYEPWGYTPLESIAYGVPTVTTDLSGFGQAILEFYGESATDSLQHGVQ
VIPRRDHDDAFVTKALATILEQATAGLPKACYKQARKTASQALWTHYYDYYIEAYRQMMT
LS

Enzyme Prediction     

EC	2.4.1.11

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT3	9	339	1.2e-81	0.5400313971742543

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd03793	GT3_GSY2-like	1.40e-54	5	485	2	531	glycogen synthase GSY2 and similar proteins. Glycogen synthase, which is most closely related to the GT3 family of glycosyltransferases, catalyzes the transfer of a glucose molecule from UDP-glucose to a terminal branch of a glycogen molecule, a rate-limit step of glycogen biosynthesis. GSY2, the member of this family in S. cerevisiae, has been shown to possess glycogen synthase activity.
pfam05693	Glycogen_syn	3.80e-51	9	485	1	526	Glycogen synthase. This family consists of the eukaryotic glycogen synthase proteins GYS1, GYS2 and GYS3. Glycogen synthase (GS) is the enzyme responsible for the synthesis of -1,4-linked glucose chains in glycogen. It is the rate limiting enzyme in the synthesis of the polysaccharide, and its activity is highly regulated through phosphorylation at multiple sites and also by allosteric effectors, mainly glucose 6-phosphate (G6P).
cd03801	GT4_PimA-like	9.84e-10	420	536	263	366	phosphatidyl-myo-inositol mannosyltransferase. This family is most closely related to the GT4 family of glycosyltransferases and named after PimA in Propionibacterium freudenreichii, which is involved in the biosynthesis of phosphatidyl-myo-inositol mannosides (PIM) which are early precursors in the biosynthesis of lipomannans (LM) and lipoarabinomannans (LAM), and catalyzes the addition of a mannosyl residue from GDP-D-mannose (GDP-Man) to the position 2 of the carrier lipid phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Glycosyltransferases catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. The acceptor molecule can be a lipid, a protein, a heterocyclic compound, or another carbohydrate residue. This group of glycosyltransferases is most closely related to the previously defined glycosyltransferase family 1 (GT1). The members of this family may transfer UDP, ADP, GDP, or CMP linked sugars. The diverse enzymatic activities among members of this family reflect a wide range of biological functions. The protein structure available for this family has the GTB topology, one of the two protein topologies observed for nucleotide-sugar-dependent glycosyltransferases. GTB proteins have distinct N- and C- terminal domains each containing a typical Rossmann fold. The two domains have high structural homology despite minimal sequence homology. The large cleft that separates the two domains includes the catalytic center and permits a high degree of flexibility. The members of this family are found mainly in certain bacteria and archaea.
pfam00534	Glycos_transf_1	5.38e-09	419	517	73	157	Glycosyl transferases group 1. Mutations in this domain of PIGA lead to disease (Paroxysmal Nocturnal haemoglobinuria). Members of this family transfer activated sugars to a variety of substrates, including glycogen, Fructose-6-phosphate and lipopolysaccharides. Members of this family transfer UDP, ADP, GDP or CMP linked sugars. The eukaryotic glycogen synthases may be distant members of this family.
pfam13692	Glyco_trans_1_4	1.19e-07	419	501	68	137	Glycosyl transferases group 1.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AEE12699.1	0.0	1	540	1	540
ATS10767.1	1.52e-166	9	540	13	546
QUI88887.1	4.32e-166	9	540	13	546
QUI90832.1	4.32e-166	9	540	13	546
ATS03592.1	6.12e-166	9	540	13	546

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QLB_A	1.84e-47	6	485	47	579	StructuralBasis for the Recruitment of Glycogen Synthase by Glycogenin [Caenorhabditis elegans],4QLB_B Structural Basis for the Recruitment of Glycogen Synthase by Glycogenin [Caenorhabditis elegans],4QLB_C Structural Basis for the Recruitment of Glycogen Synthase by Glycogenin [Caenorhabditis elegans],4QLB_D Structural Basis for the Recruitment of Glycogen Synthase by Glycogenin [Caenorhabditis elegans]
3NAZ_A	1.27e-45	4	502	27	606	Basalstate form of Yeast Glycogen Synthase [Saccharomyces cerevisiae],3NAZ_B Basal state form of Yeast Glycogen Synthase [Saccharomyces cerevisiae],3NAZ_C Basal state form of Yeast Glycogen Synthase [Saccharomyces cerevisiae],3NAZ_D Basal state form of Yeast Glycogen Synthase [Saccharomyces cerevisiae],3NCH_A Yeast Glycogen Synthase (Gsy2p) Basal State Conformation [Saccharomyces cerevisiae],3NCH_B Yeast Glycogen Synthase (Gsy2p) Basal State Conformation [Saccharomyces cerevisiae],3NCH_C Yeast Glycogen Synthase (Gsy2p) Basal State Conformation [Saccharomyces cerevisiae],3NCH_D Yeast Glycogen Synthase (Gsy2p) Basal State Conformation [Saccharomyces cerevisiae],3O3C_A Glycogen synthase basal state UDP complex [Saccharomyces cerevisiae],3O3C_B Glycogen synthase basal state UDP complex [Saccharomyces cerevisiae],3O3C_C Glycogen synthase basal state UDP complex [Saccharomyces cerevisiae],3O3C_D Glycogen synthase basal state UDP complex [Saccharomyces cerevisiae],3RSZ_A Maltodextran bound basal state conformation of yeast glycogen synthase isoform 2 [Saccharomyces cerevisiae],3RSZ_B Maltodextran bound basal state conformation of yeast glycogen synthase isoform 2 [Saccharomyces cerevisiae],3RSZ_C Maltodextran bound basal state conformation of yeast glycogen synthase isoform 2 [Saccharomyces cerevisiae],3RSZ_D Maltodextran bound basal state conformation of yeast glycogen synthase isoform 2 [Saccharomyces cerevisiae]
6U77_A	5.08e-45	4	485	7	557	yGsy2pin complex with small molecule [Saccharomyces cerevisiae S288C],6U77_B yGsy2p in complex with small molecule [Saccharomyces cerevisiae S288C],6U77_C yGsy2p in complex with small molecule [Saccharomyces cerevisiae S288C],6U77_D yGsy2p in complex with small molecule [Saccharomyces cerevisiae S288C]
5UX7_A	5.75e-45	4	485	27	577	Activatedstate yeast Glycogen Synthase in complex with UDP-xylose [Saccharomyces cerevisiae S288C],5UX7_B Activated state yeast Glycogen Synthase in complex with UDP-xylose [Saccharomyces cerevisiae S288C],5UX7_C Activated state yeast Glycogen Synthase in complex with UDP-xylose [Saccharomyces cerevisiae S288C],5UX7_D Activated state yeast Glycogen Synthase in complex with UDP-xylose [Saccharomyces cerevisiae S288C]
4KQ1_A	5.94e-45	4	485	26	576	Crystalstructure of yeast glycogen synthase in complex with uridine-5'-monophosphate [Saccharomyces cerevisiae FostersO],4KQ1_B Crystal structure of yeast glycogen synthase in complex with uridine-5'-monophosphate [Saccharomyces cerevisiae FostersO],4KQ1_C Crystal structure of yeast glycogen synthase in complex with uridine-5'-monophosphate [Saccharomyces cerevisiae FostersO],4KQ1_D Crystal structure of yeast glycogen synthase in complex with uridine-5'-monophosphate [Saccharomyces cerevisiae FostersO],4KQ2_A Glucose1,2cyclic phosphate bound activated state of Yeast Glycogen Synthase [Saccharomyces cerevisiae FostersO],4KQ2_B Glucose1,2cyclic phosphate bound activated state of Yeast Glycogen Synthase [Saccharomyces cerevisiae FostersO],4KQ2_C Glucose1,2cyclic phosphate bound activated state of Yeast Glycogen Synthase [Saccharomyces cerevisiae FostersO],4KQ2_D Glucose1,2cyclic phosphate bound activated state of Yeast Glycogen Synthase [Saccharomyces cerevisiae FostersO]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8VCB3	7.14e-50	9	485	32	556	Glycogen [starch] synthase, liver OS=Mus musculus OX=10090 GN=Gys2 PE=1 SV=2
P54840	1.33e-49	3	485	26	556	Glycogen [starch] synthase, liver OS=Homo sapiens OX=9606 GN=GYS2 PE=1 SV=2
P17625	8.94e-49	9	485	32	556	Glycogen [starch] synthase, liver OS=Rattus norvegicus OX=10116 GN=Gys2 PE=1 SV=2
Q9VFC8	2.43e-48	9	529	53	653	Glycogen [starch] synthase OS=Drosophila melanogaster OX=7227 GN=GlyS PE=1 SV=2
P13807	5.95e-48	9	485	31	556	Glycogen [starch] synthase, muscle OS=Homo sapiens OX=9606 GN=GYS1 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000042	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001209_01291.