CAZyme Information: MGYG000001210_00389

Basic Information

• Species: Mediterraneibacter sp900553885
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Mediterraneibacter; Mediterraneibacter sp900553885
• CAZyme ID: MGYG000001210_00389
• CAZy Family: GH146
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1414	MGYG000001210_19|CGC1	153979.24	4.1001

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001210	2584596	MAG	Austria	Europe

• Gene Location: Start: 11062; End: 15306 Strand: -

Full Sequence      

MYLKEKNMIELPDFSKKKGENMKSFKNWMKKGIALGAAFAMTVTVLPLSDVVNAAEPDKA
AAKAEDGLRLWYDFKGLGSGTIVNDRSGNGYAGVVRPTGSQVTTADVNIYGTDYTAYDFN
GGQPSDENTYVELPGGVFNGMEDMTVSCWVKMDYSGSMYQRIWDFGFKTDDGGFNTTSYM
YLIADGANSGHTGYTAAITNSGWGNEMGPEKGEPLETDKWIFTTVTFDGSEKEMSLYEDG
VLIGTTKTDADLSILEGADNAVIGYGQFKNDILQGQVADFKIYDYAMTADEVADQFAIPD
ADKVSRDKEWLDLGDVSAVIDNLTLPTKGAAGSDISWESSDENVIATDGTVTRPEAGKGD
ATITLTATITAGDAKDTKSFEVTVKQQLTAEEIVNGDAEDLKLSGLAAVNENLTLPTSGE
RGSTITWTSSHPDIIAADGTVTRPVGNPVDVVLTATVSYGDTSVEKEFTATVVPIYEKTD
IVKVAAVEVETQTGMLPSLPGYVDVTYENDTTGKEKVVWPTDLTVDQFSTVGDTVEVTGQ
IVDYDVTVTATVTVTDVISEAPAAVSTEFNLSDISLDGTDTIFGQNMARDLEYLKVMDAD
RMLYNFRMTFGADTKGAAPLTGWEEPTGLLRGHSTGHFLSALAQAYASTGESAYKEKMDY
MVSELHKLQQMSKGNPEDFKTKCTPTNAAQNLWSTDPSTWGEGFLSAYSPDQFALLEQYT
PYNTIWAPYYTLHKITAGMIDCYQYGGNEEALEVAKGIGSWIYRRLSNCTDEAQREKMWA
MYIAGEYGGMNESLARLYEITGEEQFMEAAKMFDHKSWFADLAANIDVVQGKHANQHIPR
IVGAIHEYAATGDVSYYNTAYNFWNIVTQRYAYSIGGVGTGEMFKDPYQQGNNILGNTGR
GENCETCAAYNMLKLTKDLYNYDPDNAEYMDYYERTVINQIAASQSHDTTDWMHNGTTYM
LPIDPGQKRDYDSDYGGFTCCNGTGMENHVKYQAAAYAKTDDTLYVNLYMPTTVTWDEMG
VSVKQETNFPSEHSKLTVNGSGDFTMKLRVPYWATAGFEVKVNGETICTNPEVSTYVEID
RAWSDGDVVEITMPYTLHLDKTPDKVDGSTVASLMYGPLVMVAKDDRETYVPMNWYTVVL
SENLEDTVEVVTGPDAEDGSVPHLVTNGLHFYPMYDAYNYRYHAYVKVEETQSVVNKDQL
QSLVDSAADALQENYGEDEWAALQQAIKDAQAVLDNADATQVDVYNAYKALQAALADTIT
PAADKSELQTAVDNALADSEKDKYTEDSWNAYQEALANAQAVLDDVNATQEDIDAALAAL
QAAQDALVPVSGTEDPNKKPGTDGQDNKPATGGDDGKGSGTDGKSNGTDGKGNGSEGPAK
AAQTGDTTPITAVAVLLVVSAALAGTVIYRKKRS

Enzyme Prediction     

No EC number prediction in MGYG000001210_00389.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH146	579	1121	2.1e-182	0.9880715705765407

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	9.93e-141	579	1120	5	501	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	1.52e-80	579	1176	15	559	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam13385	Laminin_G_3	2.22e-18	126	293	2	151	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
smart00560	LamGL	1.26e-06	217	290	60	133	LamG-like jellyroll fold domain.
pfam07532	Big_4	2.16e-05	487	544	4	59	Bacterial Ig-like domain (group 4). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF45278.1	0.0	29	1188	9	1138
AWI04506.1	1.73e-238	72	1190	64	1215
AKA69367.1	2.86e-236	109	1190	93	1215
AET60941.1	2.20e-164	389	1188	12	765
ASR47498.1	8.18e-159	389	1188	12	765

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6YQH_AAA	1.90e-79	569	1128	28	566	ChainAAA, Acetyl-CoA carboxylase, biotin carboxylase [Bacteroides thetaiotaomicron VPI-5482]
5OPJ_A	8.01e-77	569	1128	28	566	Beta-L-arabinofuranosidase[Bacteroides thetaiotaomicron]
5MQO_A	5.89e-16	794	1093	248	577	Glycosidehydrolase BT_1003 [Bacteroides thetaiotaomicron]
5HO9_A	6.75e-13	311	384	563	636	Structureof truncated AbnA (domains 1-3), a GH43 arabinanase from Geobacilllus stearothermophilus, in complex with arabinooctaose [Geobacillus stearothermophilus],5HO9_B Structure of truncated AbnA (domains 1-3), a GH43 arabinanase from Geobacilllus stearothermophilus, in complex with arabinooctaose [Geobacillus stearothermophilus]
5HO0_A	8.34e-13	311	384	563	636	Crystalstructure of AbnA (closed conformation), a GH43 extracellular arabinanase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5HO2_A Crystal structure of AbnA (open conformation), a GH43 extracellular arabinanase from Geobacillus stearothermophilus [Geobacillus stearothermophilus],5HOF_A Crystal structure of AbnA, a GH43 extracellular arabinanase from Geobacillus stearothermophilus, in complex with arabinopentaose [Geobacillus stearothermophilus],5HP6_A Structure of AbnA, a GH43 extracellular arabinanase from Geobacillus stearothermophilus (a new conformational state) [Geobacillus stearothermophilus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH9	2.95e-07	1195	1341	1736	1882	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.093718	0.900814	0.004070	0.000847	0.000316	0.000232

TMHMM  Annotations     

start	end
32	54
1387	1409