CAZyme Information: MGYG000001219_01107

Basic Information

• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; CAG-56
• CAZyme ID: MGYG000001219_01107
• CAZy Family: GH101
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2124		233310.91	4.4783

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001219	2541038	MAG	Austria	Europe

• Gene Location: Start: 330; End: 6704 Strand: +

Full Sequence      

MKSKSFSSIISLMLALVLALSGIMPYMQVSAAESSAANYDSWKIQDWGGATDIEVQDGWI
EARENGFVLNYDKIIADREKEGLVLYDSQAEQYANSVLEMDLTVSEAGDAGQVGFYSVAL
LSRFLNGANCEGIAVHDKGRLQRAGYKEGNETWEWVRNDKNDFLFGETYHLKVVTEGETL
TLSAAKKGEELQELVSLTMKTGLEASGYGFRIWRGAKKIAIENIVRTELKTDTEEGSCLN
ETEASVPDGEWGQATIQIPVTFAEGDSVQSISDGEIDLTEGSHYSVDMDNSIITISPDYM
KGQTIKDEITLTVSFVSGAQGTFKIVREASENSGWVFQKQNSSETVTPQDGWVTVSENGT
KAVIHYGKVVEDAGEGWVIFDGSAPQYKNSNLEYDLTFTDPTQGDWIAVAPVTRVTDGRN
YEGFAFTSGTGLERQSRRDGSESYAGISNLLGIKFEYNKTYHLRMETIENVITVYLTRDG
VEEKLTSFESQAGLDKGSYGFRIWRGSKTITLENIKRTEIVTSALERSTEVLSKDAWGKT
GVSIPVQFGSEDEISSIMNGDETLNPGTDYTVEDSVFTLKQEYIAAQEGTFRLQVKFAKG
SACSLWIVQRDSVAKEYTWTPDQGVDMWESVDGSGSFELAEDGKAMHVTGTNALINKLAP
IAIDGEIEITFEALRDLDGYDMGALFRADDAAGNWQAVASTDSINGEGVWDFVTPGGKSR
IVWDGVQNMSRDGVIDIKVKVRFVEDSITFWIDDQFANVCTKSQAESVMGNMGLYVDNNG
EILVKKVVFRELFPFEEAERQRETVSIANDGLTVRLDKDFPRVVDYTLNQKVMKGAKLSY
DYVTINTVDMAAEASIIAQDDRSVTYHVTPDAAKTGVTFDVKFTVLEDQILEMLILNVHE
PEDELVYSIGLPKQPLISADSTQADAMLDASYVNKNNRHFADLHKSIANKEISANDPVSV
MIPVITADGLSASMYNNVMIGGDEFVYQAFTLADGEVTVGVWNTDFMYRGVDGEKILPFP
SEPDETELYCRVAVTEDTNEDGILNWQDGANALKKLTTGIIPGSDQAQRSFFHVGYNFAS
GAQQPFLQVADNMKRLSNYIDGFSQQLVFKGYASEGHDSGHADYEDINKRAGGAEAMNVA
IAEADKIHSNFGIHINSQEIYPKAKMFNEHVAGDRDGWRWMDQSKILRRYVDMLEGGFED
RLDKLYEQTPDLDFVYVDCWGEDRWGEKKLIGTLLENGAELFGNENAADFIRFGVWTHST
SGNNNSPMNQFVYNSQRDVYPGNGIYWGGYGRSASMMSWQHRNDINGLVSQFYTNQLPQK
YLMCHEVRRVDGNTGYFDGNVTSGNYVITKDGNKITDGQGKIFIPWYDEDSETKNPDEAA
KIYHWNNDGGNTTWTLPDSWTNVTSVYLYQTTQNGKKLVGVIPVEEHQVTINASANTPYV
VYPAETKEDTTEWSVGSPLKDTGFNSRDFSIWQKSGDADIAFHDDGNGVSILTISGTEAG
AVSQTMTGLVPGQKYRVLAYAGAENGKTARITVNTPDGKTHENYLEQVIMQNNYFDNYAK
NKMVQLMWVDFIQPEGKTTAEVILSGDACEKENGKSTFMQTRIVKTAEPDLPKGYVANET
FEYIEQGAYGIFNPERAADGVPHLSETHLPYTTDTISGDWSLKMYGHYGQGNVNVRTSPA
TMRLKPNTEYSMEFDTLGGGSVYVVSEADGSDQCLESSFSKGHSSFTFTTGEKTDYIVRI
VNGSVLDNFQVYTEQAEDDITDAKQAAEAAKAAQAAAEAAQAAAEEAQAKAETAKTDAEA
AAREAEAAQAKAEAAQAAAEEAAAQADADKAVVEQAKKEAEAAKEAAETAKQNAKESADQ
AETAREAAETAQRKAETAKNAAETAKEAAEAARDGAVEAKDQAEAARDAAENARDEAQSA
CDSASTAQAAAESAKESAIAQAGLAEAAKNAAEAAQALAEAAQRAAEKARDEAAEILKKA
QEEFAKAEDARKKAQSEADDKLARAEAMLKEAMELEKQTKDYVQEATFKAQRVTITSAKS
KKKKTAKITWKKVDGAEGYVIEYALKANFKGAKAVTIRKGTTGSRMLKKLKSRKAYYVRV
KAYKTVNGKKIFTDIGAKKKVRVK

Enzyme Prediction     

No EC number prediction in MGYG000001219_01107.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH101	812	1331	2.6e-88	0.7878359264497878

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam12905	Glyco_hydro_101	3.05e-64	1047	1307	1	271	Endo-alpha-N-acetylgalactosaminidase. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by the S. pneumoniae protein Endo-alpha-N-acetylgalactosaminidase, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor.
cd14244	GH_101_like	8.83e-60	1062	1323	2	289	Endo-a-N-acetylgalactosaminidase and related glcyosyl hydrolases. This family contains the enzymatically active domain of cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins (EC:3.2.1.97). It has been classified as glycosyl hydrolase family 101 in the Cazy resource. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae and other commensal human bacteria is largely determined by their ability to degrade host glycoproteins and to metabolize the resultant carbohydrates.
pfam18080	Gal_mutarotas_3	2.38e-35	807	1046	1	243	Galactose mutarotase-like fold domain. This domain is found in endo-alpha-N-acetylgalactosaminidase present in Streptococcus pneumoniae. Endo-alpha-N-acetylgalactosaminidase is a cell surface-anchored glycoside hydrolase involved in the breakdown of mucin type O-linked glycans. The domain, known as domain 2, exhibits strong structural similarlity to the galactose mutarotase-like fold but lacks the active site residues. Domains, found in a number of glycoside hydrolases, structurally similar to domain 2 confer stability to the multidomain architectures.
pfam17451	Glyco_hyd_101C	4.61e-22	1317	1416	3	110	Glycosyl hydrolase 101 beta sandwich domain. Virulence of pathogenic organisms such as the Gram-positive Streptococcus pneumoniae is largely determined by the ability to degrade host glycoproteins and to metabolize the resultant carbohydrates. This family is the enzymatic region, EC:3.2.1.97, of the cell surface proteins that specifically cleave Gal-beta-1,3-GalNAc-alpha-Ser/Thr (T-antigen, galacto-N-biose), the core 1 type O-linked glycan common to mucin glycoproteins. This reaction is exemplified by a S. pneumoniae protein, where Asp764 is the catalytic nucleophile-base and Glu796 the catalytic proton donor. This domain represents C-terminal the beta sandwich domain.
pfam03442	CBM_X2	2.74e-08	540	602	16	78	Carbohydrate binding domain X2. This domain binds to cellulose and to bacterial cell walls. It is found in glycosyl hydrolases and in scaffolding proteins of cellulosomes (multiprotein glycosyl hydrolase complexes). In the cellulosome it may aid cellulose degradation by anchoring the cellulosome to the bacterial cell wall and by binding it to its substrate. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ATD58436.1	1.49e-162	544	1740	422	1608
ATD54117.1	1.49e-162	544	1740	422	1608
QBJ76357.1	1.49e-162	544	1740	422	1608
SLK22612.1	1.49e-162	544	1740	422	1608
BBK62189.1	1.24e-143	337	1770	50	1493

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6QEP_A	5.03e-99	806	1710	11	1043	EngBFDARPin Fusion 4b H14 [Bifidobacterium longum]
6QFK_A	5.20e-99	806	1710	11	1043	EngBFDARPin Fusion 4b G10 [Bifidobacterium longum]
6QEV_B	5.20e-99	806	1710	11	1043	EngBFDARPin Fusion 4b B6 [Bifidobacterium longum]
6SH9_B	5.20e-99	806	1710	11	1043	EngBFDARPin Fusion 4b D12 [Bifidobacterium longum subsp. longum JCM 1217]
2ZXQ_A	6.39e-99	806	1710	26	1058	Crystalstructure of endo-alpha-N-acetylgalactosaminidase from Bifidobacterium longum (EngBF) [Bifidobacterium longum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A9WNA0	1.53e-84	805	1695	50	962	Putative endo-alpha-N-acetylgalactosaminidase OS=Renibacterium salmoninarum (strain ATCC 33209 / DSM 20767 / JCM 11484 / NBRC 15589 / NCIMB 2235) OX=288705 GN=RSal33209_1326 PE=3 SV=2
Q2MGH6	1.72e-79	806	1699	333	1323	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=SP_0368 PE=1 SV=1
Q8DR60	3.90e-79	806	1699	333	1323	Endo-alpha-N-acetylgalactosaminidase OS=Streptococcus pneumoniae (strain ATCC BAA-255 / R6) OX=171101 GN=spr0328 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000381	0.998791	0.000189	0.000259	0.000188	0.000157

TMHMM  Annotations     

start	end
7	29