CAZyme Information: MGYG000001225_00502

Basic Information

• Species: Evtepia sp004556345
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Oscillospiraceae; Evtepia; Evtepia sp004556345
• CAZyme ID: MGYG000001225_00502
• CAZy Family: GH18
• CAZyme Description: Spore germination protein YaaH

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
384	MGYG000001225_2|CGC2	42474.1	4.4768

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001225	2156810	MAG	Austria	Europe

• Gene Location: Start: 199216; End: 200370 Strand: +

Full Sequence      

MIYVVAPGDTLSGIARLSGSSVAQLQQDNGILPDQPLVPGQALVVPDRDGTASEKPLLSV
GGYAYPHIRPGVLQQALPFLTTLSIFSYGFREDGTLVEPPARWLLQQAGEVGAGAVLVLT
STDEDGNFSSQRAAQLFRDRVLQTQVLDSLLQVLIEKGYVGLDADFEYIPETDRDAFFAF
LDNARERLHQYGFFLQVDLAPKTYAQQPGLLYVAHDYAVIGSIADTVLLMTYEWGYAYGP
PMAIAPLPHVEAVVRYAVTEIPTWKIQLGIPNYGYDWTLPYEPGRRAVTLGNEEAVRLAA
QVGAEIQFDPVSQAPTFQYQTAGTIHQVWFEDARSVQAKFDLIERNQLVGGTYWNLLRPF
PQNWALAAQRITPRSLWQGSLQSP

Enzyme Prediction     

No EC number prediction in MGYG000001225_00502.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	127	356	4.5e-32	0.7027027027027027

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02874	GH18_CFLE_spore_hydrolase	9.42e-113	72	364	18	310	Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
COG3858	YaaH	1.63e-90	2	364	51	415	Spore germination protein YaaH [Cell cycle control, cell division, chromosome partitioning].
cd06549	GH18_trifunctional	4.20e-27	92	335	37	269	GH18 domain of an uncharacterized family of bacterial proteins, which share a common three-domain architecture: an N-terminal glycosyl hydrolase family 18 (GH18) domain, a glycosyl transferase family 2 domain, and a C-terminal polysaccharide deacetylase domain.
smart00636	Glyco_18	3.96e-22	148	356	97	331	Glyco_18 domain.
pfam00704	Glyco_hydro_18	2.23e-14	144	356	90	304	Glycosyl hydrolases family 18.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QEK18230.1	1.61e-118	2	366	3	368
ASB39292.1	3.83e-118	1	370	2	419
QQR28581.1	3.83e-118	1	370	2	419
ANU54026.1	3.83e-118	1	370	2	419
QYX28648.1	7.20e-113	1	371	2	375

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4S3K_A	3.70e-72	72	374	127	432	ChainA, Spore germination protein YaaH [Priestia megaterium QM B1551]
4S3J_A	9.54e-72	2	372	5	426	Crystalstructure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_B Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_C Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876]
3CZ8_A	2.01e-46	66	356	19	308	ChainA, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168],3CZ8_B Chain B, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168]
5JH8_A	7.23e-23	119	374	67	317	Crystalstructure of chitinase from Chromobacterium violaceum ATCC 12472 [Chromobacterium violaceum ATCC 12472]
4Q6T_A	1.92e-17	87	354	37	322	Thecrystal structure of a class V chitininase from Pseudomonas fluorescens Pf-5 [Pseudomonas protegens Pf-5]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P37531	1.15e-72	2	371	3	423	Cortical fragment-lytic enzyme OS=Bacillus subtilis (strain 168) OX=224308 GN=sleL PE=1 SV=2
P0DPJ9	5.29e-70	2	372	4	425	Cortical fragment-lytic enzyme OS=Bacillus anthracis OX=1392 GN=sleL PE=2 SV=1
Q9K3E4	5.29e-70	2	372	4	425	Cortical fragment-lytic enzyme OS=Bacillus cereus OX=1396 GN=sleL PE=1 SV=1
O05495	2.00e-53	2	366	3	411	Putative sporulation-specific glycosylase YdhD OS=Bacillus subtilis (strain 168) OX=224308 GN=ydhD PE=1 SV=2
O32258	2.04e-21	79	356	51	328	Uncharacterized glycosylase YvbX OS=Bacillus subtilis (strain 168) OX=224308 GN=yvbX PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000069	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001225_00502.