CAZyme Information: MGYG000001237_00497

Basic Information

• Species: UMGS1600 sp900553295
• Lineage: Bacteria; Firmicutes_A; Clostridia_A; Christensenellales; CAG-74; UMGS1600; UMGS1600 sp900553295
• CAZyme ID: MGYG000001237_00497
• CAZy Family: GT39
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1389		155940.86	5.8376

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001237	2708995	MAG	Austria	Europe

• Gene Location: Start: 13259; End: 17428 Strand: +

Full Sequence      

MYKRVLLCLMLCCCLAMCFAASLAEEAEENLLYNGSFEIIGADGMPDGWYTDQYNRQEGY
TNFAVLTGDAQEGEHSVQIENLGDNDARFAQRVEVKPESLYCLSGYIRAEQIPDSGLGAN
LSVEGLYVFSESVYDTAGEWQYVELYGETGPEQTEVTVFARLGGYSGESRGTAAFDNLSL
REVNIVPGDGVASLWFEPDVQETVIEPVQEGEVDPFWPWLLVISALYALAALVACDVMMR
RREKEPLVPMGKKKHAPAFLVVALIAAALARVVDACLVSGYQVDVNCFRAWGASMTQLGP
AGFYAADQFCDYPPAYLYVLWLDNGIINLLNGITPAGFASWFATLGIGSYYDVMSVLVIK
LVPMACDLGASYLLYRVGREKLGDRPAAVLAALMAFNPAFFLNSAAWCQMDSVLCLLLAL
VALTAMRRNWRAALPLYMLCVLVKPQALMLGFLGLLAMGIVWAKHREDRRAMLQGAGIAV
LTALVVVLPFSLGSGLNWNWLFTLYSNTLASYSYATVNMANLYYLFDGNWVSTTTSAGWQ
LPVAFALLCATWSAVTWIRQRGKLRWFWAEPAAMACFTVYYLVAAFVQPAYTWLGVPAMA
LCILLTLGMYLRGGRLENLPLAGGMLFMLLCVFGLKMHERYLFPALLFFALAFLQHRDWR
ILLLMICGTLTIFVNAGIVLDNSLRLGSSMGHLNNDTLWLNDLISLVNVLSALLAVWTGQ
RVMVENQPQQAHGGLRLGKPVQLPAKPGNVLDLRYDAGLHWKRVDAVLVAAVTLVYGALA
LCNLGSTKAPQNPWKSTDATEQVVIDLGAHYDDFRMLYFAQVSYSDFSVAVSEDGELWSE
EYWAQMDQGQCFRWMYLTPYTVNANGQRTYDGYGTPRSLSGRYVRITAQQIGLILDEVIF
RLEDQTVLPAQVVSRVHANEASTLFSDPENLLDEQDTLEGEPSWYNSTYFDEIYHARTAF
ELLNGTSVYEWTHPPLGKVLMSWCIALFGMTPFGWRFAGCMAGILMLPFMYLLAKQLTKR
RDLAFAAMALLALDCMHLTQTRIATIDSFPVLFIMMSFFFMLRFMQRDLMAGMKPMLTDL
AWSGVSIGLGIASKWIGLYAGAGLAVLFFWTCWRHIRLSYQAKELLSTAGGLSKDQREAL
IRRSRETLTRLVWLCLWCVVFFILVPVAIYLLSYIPQMACYGKLSLRDFLEKVIRVQQDM
YNYHSTPGLGMDHPFYSPWYEWLFIKRPMYFASDRYVPAGYGYAIFCFGNPAVWIGGLVG
ILVCALIWCRGHVYEIQGQEGALHWQSMVSHKYAFVIIGLLAQLLPWILVPRGTYIYHYF
ASVPFLVLGATLTIEWIQQGRWPRLGRALTVAYLVFALICFIGFYPYASGVTTPTWWLDF
MKQFLLIYY

Enzyme Prediction     

No EC number prediction in MGYG000001237_00497.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT39	946	1176	6.4e-53	0.9103139013452914

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam16192	PMT_4TMC	5.87e-18	1187	1366	1	179	C-terminal four TMM region of protein-O-mannosyltransferase. PMT_4TMC is the C-terminal four membrane-pass region of protein-O-mannosyltransferases and similar enzymes.
COG1928	PMT1	1.56e-16	944	1174	44	264	Dolichyl-phosphate-mannose--protein O-mannosyl transferase [Posttranslational modification, protein turnover, chaperones].
pfam02366	PMT	1.79e-14	943	1114	16	202	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
pfam13231	PMT_2	4.62e-08	973	1120	2	142	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
COG5542	COG5542	1.07e-04	309	523	92	278	Mannosyltransferase related to Gpi18 [Carbohydrate transport and metabolism].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUA53848.1	0.0	2	1389	8	1372
QTE68569.1	0.0	5	1389	12	1402
QTE71236.1	0.0	17	1389	1	1407
QTE75201.1	0.0	17	1389	16	1422
QUC68008.1	0.0	3	1389	5	1422

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6P25_A	2.51e-06	943	1113	69	253	Structureof S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor and a peptide acceptor [Saccharomyces cerevisiae W303],6P2R_A Structure of S. cerevisiae protein O-mannosyltransferase Pmt1-Pmt2 complex bound to the sugar donor [Saccharomyces cerevisiae W303]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
L8F4Z2	1.11e-22	940	1376	57	506	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycolicibacterium smegmatis (strain MKD8) OX=1214915 GN=pmt PE=3 SV=1
P9WN04	3.64e-22	950	1379	73	515	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=pmt PE=3 SV=2
P9WN05	3.64e-22	950	1379	73	515	Probable dolichyl-phosphate-mannose--protein mannosyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=pmt PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000399	0.998760	0.000256	0.000185	0.000192	0.000178

TMHMM  Annotations     

start	end
5	24
216	235
256	273
326	348
353	375
399	421
434	456
471	493
500	522
537	559
566	585
589	611
618	635
640	654
661	680
992	1014
1043	1065
1085	1107
1151	1173
1241	1263
1293	1310
1315	1337
1349	1371