CAZyme Information: MGYG000001248_00943

Basic Information

• Species: Dialister sp900543165
• Lineage: Bacteria; Firmicutes_C; Negativicutes; Veillonellales; Dialisteraceae; Dialister; Dialister sp900543165
• CAZyme ID: MGYG000001248_00943
• CAZy Family: GT83
• CAZyme Description: Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
556	MGYG000001248_16|CGC1	63451.45	8.543

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001248	2225809	MAG	Austria	Europe

• Gene Location: Start: 40170; End: 41840 Strand: +

Full Sequence      

MSTKRQKHERKQAALAKENERSTLISFISFFIIAFLFFLWGSWLLPITDPVESNYALTAR
EMVESGDWMSPQIYGVYWYDKPIMVYWFLSLAYSALGFTDLASRLPSALAGALSVTLLIW
YLRRILKDNVVSIWAGVMLATSLECWVISHAIITDSLLLLFTIPTMLSAYIGITENSKKH
LVIAYAGAGLACLTKGPVGLVLPGLLLVLWCLSMKSPKTILRLFLWQGLLAFFVIVLPWY
GGMYAIHGSDFINEFLGLHNVLRATSSEHPEDNHWYYYLILLPASLLPWAFVSFYEMKMR
WKEKGAFYLFLMVWCWGTILFYTLMATKYVTYSYIAVVPAITFAAFGALRIRMGEKLPSI
LGGLGLLLLMAALFAGTFRLKEGNWLVFYAVLAYGLFAYLIRWKANQYKRLTIISAVTAS
AFLCCISEGLPHFMMTRSAFEIESAVHEEKGKHYFFLTYPASYSYYTGDIATRLMPDEPL
LDQEKRDARWDKKSPIPTVNEEQFIEAAKASDAPSYLYVANEKAKFFDTWSLAPRFEKVE
STKTGTIYRFVETSDW

Enzyme Prediction     

No EC number prediction in MGYG000001248_00943.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT83	24	484	6.2e-92	0.8962962962962963

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG1807	ArnT	1.17e-37	40	351	23	339	4-amino-4-deoxy-L-arabinose transferase or related glycosyltransferase of PMT family [Cell wall/membrane/envelope biogenesis].
PRK13279	arnT	2.79e-25	50	332	31	317	lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase.
pfam13231	PMT_2	2.86e-16	80	239	1	157	Dolichyl-phosphate-mannose-protein mannosyltransferase. This family contains members that are not captured by pfam02366.
pfam02366	PMT	7.66e-12	52	247	32	240	Dolichyl-phosphate-mannose-protein mannosyltransferase. This is a family of Dolichyl-phosphate-mannose-protein mannosyltransferase proteins EC:2.4.1.109. These proteins are responsible for O-linked glycosylation of proteins, they catalyze the reaction:- Dolichyl phosphate D-mannose + protein <=> dolichyl phosphate + O-D-mannosyl-protein. Also in this family is Drosophila rotated abdomen protein which is a putative mannosyltransferase. This family appears to be distantly related to pfam02516 (A Bateman pers. obs.). This family also contains sequences from ArnTs (4-amino-4-deoxy-L-arabinose lipid A transferase). They catalyze the addition of 4-amino-4-deoxy-l-arabinose (l-Ara4N) to the lipid A moiety of the lipopolysaccharide. This is a critical modification enabling bacteria (e.g. Escherichia coli and Salmonella typhimurium) to resist killing by antimicrobial peptides such as polymyxins. Members such as undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase are predicted to have 12 trans-membrane regions. The N-terminal portion of these proteins is hypothesized to have a conserved glycosylation activity which is shared between distantly related oligosaccharyltransferases ArnT and PglB families.
COG4745	COG4745	4.93e-04	75	351	56	375	Predicted membrane-bound mannosyltransferase [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK24579.1	1.23e-239	1	548	1	548
AOH39318.1	2.23e-177	18	555	7	541
AXB83023.1	7.85e-121	32	517	12	504
QQB17174.1	1.02e-115	31	548	11	534
SNV02078.1	1.02e-115	31	548	11	534

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5EZM_A	5.67e-18	47	290	54	300	CrystalStructure of ArnT from Cupriavidus metallidurans in the apo state [Cupriavidus metallidurans CH34],5F15_A Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate [Cupriavidus metallidurans CH34]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B4TBG8	8.45e-27	49	366	30	349	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Salmonella heidelberg (strain SL476) OX=454169 GN=arnT PE=3 SV=1
Q57M54	5.13e-25	49	366	29	348	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Salmonella choleraesuis (strain SC-B67) OX=321314 GN=arnT PE=3 SV=2
B7LM74	2.31e-23	31	347	6	332	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CCUG 18766 / IAM 14443 / JCM 21226 / LMG 7866 / NBRC 102419 / NCTC 12128 / CDC 0568-73) OX=585054 GN=arnT PE=3 SV=2
A4SQX1	1.72e-22	46	332	24	314	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Aeromonas salmonicida (strain A449) OX=382245 GN=arnT PE=3 SV=1
A9MJC5	5.47e-22	49	366	30	349	Undecaprenyl phosphate-alpha-4-amino-4-deoxy-L-arabinose arabinosyl transferase OS=Salmonella arizonae (strain ATCC BAA-731 / CDC346-86 / RSK2980) OX=41514 GN=arnT PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999995	0.000043	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
25	47
76	98
105	122
132	149
156	173
188	210
223	245
275	294
306	325
329	351
358	380
384	401
413	435