CAZyme Information: MGYG000001253_01774

Basic Information

• Species: Paramuribaculum sp001689535
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; Paramuribaculum; Paramuribaculum sp001689535
• CAZyme ID: MGYG000001253_01774
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
859		97089.77	6.4715

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001253	2271514	MAG	Austria	Europe

• Gene Location: Start: 8004; End: 10583 Strand: +

Full Sequence      

MKSVIILSSILISSLMTNASVITQQLHSGWKFRQNNLMQWHPATVPGTVHTDLMASGMIP
DPFIAQNERMVQWVDKEDWLYSTTFDLDPTVGAADSVSLVFDGLDTYADVFLNDSLILKA
DNMFRRWEVPVASLLKPSGNDLRILFHSPLKTDIPKYEALGLKYPYPASNDQSENGGVFN
KKVSVFARKAGYHYGWDWGPRLVTSGIWRPVYLKAWSGPVIDDVFIRTLSASGKEASMTA
TVEINSPALVKGCTVTVGGEKTYARTKTDLMPGLNRIEIPFTVKNPRLWWCNGLGSPELY
RFDVKISAGHNIAATRSVTAGIRTIELVRDDDAQGRTFYFRLNGRPVFAKGANYIPSDNF
LPRVTTEQYRRTIADAARANMNMLRVWGGGIYEDDRFYSLCDSMGIMVWQDFMFACSMYP
AEGALLDNIRHEAVDNVRRLRNHPSIALWCGNNECLDAWYGWGWRRRAEGAGEADMMRRQ
FDDVYFALLPQTVAEHDPDRPYTPSSPFSRPDGPSQAERGDFHFWSVWGGGAPIELYNKQ
RSRFFSEYGFQSFPEIETVRKYAPDSASHFIDSDVMLAHQRAGAGANRKLEKYLLEGYPE
PKDFESMLYMTQVLQGDAIRTAIEAHRRDRPYCMGSLFWQHNDCWPVASWSSRDYYGRWK
AQHYFAAKAYRDIMLSLRPDSLGAITLYIASDRKEAVQAHVDIDMLSLDGHILTSLDRQV
NVQPDSVTVLSIPASDLAEGIDPRDAVITASLRTPDDSTYETLLLLAPQKEVRFPQAVIT
CGVSGSDGDYTVSVKSDKFVRAFRMTLGDGDYFFADNYFDLLPDREYRVALHAPGMSAAE
VRQRLSHTYLHHASKRSEN

Enzyme Prediction     

EC	3.2.1.25

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	21	804	5.7e-115	0.7712765957446809

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	3.43e-110	42	761	31	717	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam00703	Glyco_hydro_2	3.48e-12	221	323	3	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.
PRK10150	PRK10150	1.91e-11	30	503	19	446	beta-D-glucuronidase; Provisional
PRK10340	ebgA	8.00e-11	204	478	194	470	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam17786	Mannosidase_ig	8.93e-11	683	771	1	91	Mannosidase Ig/CBM-like domain. This domain corresponds to domain 4 in the structure of Bacteroides thetaiotaomicron beta-mannosidase, BtMan2A. This domain has an Ig-like fold.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDO68488.1	0.0	8	832	11	844
ALJ60279.1	0.0	1	829	8	841
QUT88714.1	0.0	1	829	8	841
QUT74423.1	0.0	1	832	4	843
QGT72566.1	0.0	18	826	29	840

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VJX_A	2.06e-217	26	840	11	825	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VJX_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VL4_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VMF_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VO5_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VOT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron VPI-5482],2VQT_A Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQT_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VR4_A Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482],2VR4_B Transition-state mimicry in mannoside hydrolysis: characterisation of twenty six inhibitors and insight into binding from linear free energy relationships and 3-D structure [Bacteroides thetaiotaomicron VPI-5482]
2JE8_A	2.19e-217	26	840	13	827	Structureof a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JE8_B Structure of a beta-mannosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
7OP6_A	2.26e-217	26	840	13	827	ChainA, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP6_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_A Chain A, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482],7OP7_B Chain B, Beta-mannosidase [Bacteroides thetaiotaomicron VPI-5482]
2WBK_A	5.99e-217	26	840	11	825	Structureof the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482],2WBK_B Structure of the Michaelis complex of beta-mannosidase, Man2A, provides insight into the conformational itinerary of mannoside hydrolysis [Bacteroides thetaiotaomicron VPI-5482]
2VQU_A	2.61e-215	26	840	11	825	Structuraland biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron],2VQU_B Structural and biochemical evidence for a boat-like transition state in beta-mannosidases [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q4WAH4	5.72e-137	26	730	9	721	Beta-mannosidase B OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=mndB PE=3 SV=1
A1CGA8	1.58e-136	26	831	9	828	Beta-mannosidase B OS=Aspergillus clavatus (strain ATCC 1007 / CBS 513.65 / DSM 816 / NCTC 3887 / NRRL 1 / QM 1276 / 107) OX=344612 GN=mndB PE=3 SV=1
B0YBU9	2.22e-136	26	730	9	721	Beta-mannosidase B OS=Neosartorya fumigata (strain CEA10 / CBS 144.89 / FGSC A1163) OX=451804 GN=mndB PE=3 SV=1
A1D911	1.21e-135	26	730	9	721	Beta-mannosidase B OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=mndB PE=3 SV=1
B8NW36	5.56e-132	26	752	9	748	Beta-mannosidase B OS=Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC 167) OX=332952 GN=mndB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000422	0.998850	0.000214	0.000175	0.000165	0.000157

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001253_01774.