CAZyme Information: MGYG000001257_00413

Basic Information

• Species: Massiliomicrobiota sp002160865
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelatoclostridiaceae; Massiliomicrobiota; Massiliomicrobiota sp002160865
• CAZyme ID: MGYG000001257_00413
• CAZy Family: GT2
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
753		88661.81	7.103

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001257	2104226	MAG	Austria	Europe

• Gene Location: Start: 1042; End: 3303 Strand: +

Full Sequence      

MDAYLFFHIIYKKSGFSMIKKSNIIFILSFIFSSLYLIWRTFWTLPLNQSMGEIIFAVIL
LLSEIITTFTSFELIIHKMRNDEKRLVPPVLQAQDYPEIDIFIATHNESVDLLYKTVNAC
TFLEYPDLQLVHIYICDDQNRPEVKHLAEHFGVGYIGLEYNQHAKAGNFNHALSMTHSPL
IVTFDADMIPERTFLMKTVPYFFMKDTKTSSYLPIALVQTPQSFYNQDLFQFHLFSETTI
PNEQDFFSKEINVLRNAHFGASYTGSNAVILREALEKIGGFPYHTVTEDFETSIRLQKAG
YWTYSTQESLASGLSTTTIPSMIQQRIRWAQGVIQSIYNTHACTTSSLSLGLRLSYLCAF
LYWWSFLTRLIFILAPILFALFDLQFVQCHFGELLIFWLPSSIFYHLSLNALSTQTRNQR
WSQIIDTILAPFLIIPVLLETLGIHQKIFKVTNKKKATMHTRSLFYMIPHLILVILTILA
IIRYGMGKYGSALFYSSVILFWLFYNLVTLCFAIFFMLGRPSYRAYERFSIQEAIDIHFH
HQTYQGMSVDFSYVSLSFSLPLPFYVPADIPFYIEIKTDQYQTTLPVQLLYVKVVQEQWV
YVVLLQDTSEEDYRQYLQIIHDRKHSLPSHIDEWSSLYDDLYRNIQSRFQKRQQEQRQTF
RIPVEQVFHLTNGMEIFVVDFNYHYLLVKDFQGDKQKEYCLNWYGMIFQLRFKKQVNHQL
CLLEVINFEELFFNKKALKVFLQHLIYTKVDET

Enzyme Prediction     

No EC number prediction in MGYG000001257_00413.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	100	330	3.4e-20	0.9695652173913043

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06421	CESA_CelA_like	2.74e-80	97	335	1	232	CESA_CelA_like are involved in the elongation of the glucan chain of cellulose. Family of proteins related to Agrobacterium tumefaciens CelA and Gluconacetobacter xylinus BscA. These proteins are involved in the elongation of the glucan chain of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues. They are putative catalytic subunit of cellulose synthase, which is a glycosyltransferase using UDP-glucose as the substrate. The catalytic subunit is an integral membrane protein with 6 transmembrane segments and it is postulated that the protein is anchored in the membrane at the N-terminal end.
PRK11498	bcsA	2.17e-70	24	510	188	669	cellulose synthase catalytic subunit; Provisional
COG1215	BcsA	6.94e-44	43	510	5	427	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
cd06435	CESA_NdvC_like	6.37e-28	101	342	2	236	NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. NdvC_like proteins in this family are putative bacterial beta-(1,6)-glucosyltransferase. Bradyrhizobium japonicum synthesizes periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans during growth under hypoosmotic conditions. Two genes (ndvB, ndvC) are involved in the beta-(1, 3), beta-(1,6)-glucan synthesis. The ndvC mutant strain resulted in synthesis of altered cyclic beta-glucans composed almost entirely of beta-(1, 3)-glycosyl linkages. The periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans function for osmoregulation. The ndvC mutation also affects the ability of the bacteria to establish a successful symbiotic interaction with host plant. Thus, the beta-glucans may function as suppressors of a host defense response.
cd06423	CESA_like	6.65e-18	101	289	1	180	CESA_like is the cellulose synthase superfamily. The cellulose synthase (CESA) superfamily includes a wide variety of glycosyltransferase family 2 enzymes that share the common characteristic of catalyzing the elongation of polysaccharide chains. The members include cellulose synthase catalytic subunit, chitin synthase, glucan biosynthesis protein and other families of CESA-like proteins. Cellulose synthase catalyzes the polymerization reaction of cellulose, an aggregate of unbranched polymers of beta-1,4-linked glucose residues in plants, most algae, some bacteria and fungi, and even some animals. In bacteria, algae and lower eukaryotes, there is a second unrelated type of cellulose synthase (Type II), which produces acylated cellulose, a derivative of cellulose. Chitin synthase catalyzes the incorporation of GlcNAc from substrate UDP-GlcNAc into chitin, which is a linear homopolymer of beta-(1,4)-linked GlcNAc residues and Glucan Biosynthesis protein catalyzes the elongation of beta-1,2 polyglucose chains of Glucan.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUN12766.1	0.0	18	753	1	736
QLG37677.1	5.02e-241	20	731	5	738
SMF90406.1	2.86e-240	20	731	5	738
QZN77042.1	2.30e-239	20	694	5	696
QNK55597.1	2.82e-239	20	731	5	745

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7LBY_A	1.42e-51	96	510	272	682	ChainA, Cellulose synthase catalytic subunit [UDP-forming] [Escherichia coli K-12]
5EJ1_A	1.14e-47	36	454	68	496	ChainA, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]
4HG6_A	2.12e-47	36	454	80	508	ChainA, Cellulose Synthase Subunit A [Cereibacter sphaeroides]
4P00_A	2.14e-47	36	454	81	509	ChainA, Cellulose Synthase A subunit [Cereibacter sphaeroides 2.4.1],4P02_A Chain A, Cellulose Synthase subunit A [Cereibacter sphaeroides 2.4.1],5EIY_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1],5EJZ_A Chain A, Putative cellulose synthase [Cereibacter sphaeroides 2.4.1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q93IN2	1.73e-53	87	510	263	682	Cellulose synthase catalytic subunit [UDP-forming] OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=bcsA PE=3 SV=1
Q8Z291	4.82e-52	87	510	263	682	Cellulose synthase catalytic subunit [UDP-forming] OS=Salmonella typhi OX=90370 GN=bcsA PE=3 SV=1
Q8X5L7	2.89e-51	96	510	272	682	Cellulose synthase catalytic subunit [UDP-forming] OS=Escherichia coli O157:H7 OX=83334 GN=bcsA PE=3 SV=2
P37653	7.12e-51	96	510	272	682	Cellulose synthase catalytic subunit [UDP-forming] OS=Escherichia coli (strain K12) OX=83333 GN=bcsA PE=1 SV=3
Q9WX75	5.78e-50	28	454	78	495	Putative cellulose synthase 3 OS=Komagataeibacter xylinus OX=28448 GN=bcsABII-B PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000047	0.000005	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
22	44
54	76
360	382
422	444
465	487
497	519