CAZyme Information: MGYG000001267_00220

Basic Information

• Species: Lancefieldella sp902373375
• Lineage: Bacteria; Actinobacteriota; Coriobacteriia; Coriobacteriales; Atopobiaceae; Lancefieldella; Lancefieldella sp902373375
• CAZyme ID: MGYG000001267_00220
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1273	MGYG000001267_4|CGC2	140508.55	5.4362

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001267	1280195	MAG	Italy	Europe

• Gene Location: Start: 43017; End: 46838 Strand: +

Full Sequence      

MKLQVTEQMNSAPETVCMSSVTDASARVQHLNTGWKFKLGNASGAENIFYDDSLWDLVNL
PHDFSINQPFTTSGEAQSAYKLGGEAWYRKTFQVDQECKNKCFRLDFDGVYMDSTVWVNG
HLLGTHPYGYSPFAFDITQYIKFSEYNTITVRVNAQIPNSRWYSGAGIGRDVDLIVTRPV
YVQKDGVKIATPNLASELGSNVSVQLTTAVVNSLRTAASVQIKQIIYLAGKAPEQKIASA
NTERRMGAGSTDSFTVTAITTEAPKLWDITSPHLYVVRTQVLVDKNIVDTYETTFGFRFF
SFDAQKGFSLNGKHIKIKGVCLHQDQGALGSVSYADAVNRQIQILKQMGVNAIRTSHNTP
CKELVAACNTQGMLLDYEFFDGWTSHKNGNSNDYARFFSKEMGESELIGSKSSKTWAQFD
LESSINRDYNAPSIVMWSLGNEMTEGTLGISHLPQVQADLIAWAKAADQSRPITTADNQL
RLSSSELNPQAIADANGLVGLNYAGGDIYDWLHNLHPDWKLMGSETASSVNSRGIYHTYG
IDSASQQLSAYDFSCVEWGHFASQAWYDVLIRDFIAGEFVWTGFDYLGEPTPWNGIDSGA
KGRWPSPKNSYFGIIDTAGLPKDSYYFYQSQWNDSVHTLHLLPAWNNNVIHKNPDGTVDV
VVYTDAQAVRLYFTQKGSTERKDLGLKSFTTKSTPTNGFSYKIYEGPDKSNDEFKNLYLT
WKVPYAEGVISAEAYDEAGNVLDTSSWEGRQALITTGYPKKLIASPNRETLSANGSDLLY
VTVDIVDKDGNRVPNADNKVTFEVSGAGRLVGLDNGSSPDHQSYQDNNRNAFSGQLVGIV
QAAEVPGKIAIRISSEGLESAVVTIPVSAVDSKKTYQKEVKTLLYSRFLYVKLGSSVVLP
QRVKVCYIDGTVTDEKVIWDDFDAEKCNSVGTFVIYGTVAGARISVVVVVLNNIVALLNY
SATTPVGQKPQLPDARPAIQADGTILQTLLPVTWEAVPKDAFDHEGTVVITGRAHVFGSD
LLVTASIRIQKEQLMLGQDIASSASVLLNGLEEVRHDSPDTKEAQDQKDDIAYRISYEQV
QKKHELSSITYAYATQQRFGYAKIHFFAGSDETKLPKPGTIALEISENGRDWIAVDVQET
TGPVSQKTIVYTYDFSPITATYIRISFKGDDEVLTSAGEYIGIVQIELFSISGSYTSYST
TQLASLVVNGKNIDQKAIKEKTYATPKRLTSVEASTKENSSLTILPPYHNKIRLLLESED
HTARDAFILTVKA

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	12	667	8.1e-117	0.6821808510638298

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	8.04e-67	19	734	3	639	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	1.05e-42	764	864	3	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	5.17e-34	35	474	19	446	beta-D-glucuronidase; Provisional
PRK10340	ebgA	2.12e-32	23	442	36	449	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam02837	Glyco_hydro_2_N	1.22e-23	28	177	1	168	Glycosyl hydrolases family 2, sugar binding domain. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities and has a jelly-roll fold. The domain binds the sugar moiety during the sugar-hydrolysis reaction.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ACV50538.1	0.0	4	1271	40	1316
QWT18242.1	0.0	6	1261	41	1301
QWT17379.1	0.0	8	1267	75	1356
SQH28248.1	0.0	8	1271	51	1322
ADH92997.1	0.0	8	1271	51	1322

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5DMY_A	0.0	6	890	16	904	Beta-galactosidase- construct 33-930 [Bifidobacterium bifidum],5DMY_B Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum],5DMY_C Beta-galactosidase - construct 33-930 [Bifidobacterium bifidum]
6QUB_A	0.0	6	875	10	884	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum],6QUC_A Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUC_B Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum],6QUD_A 2-deoxy-galactose reaction intermediate of a Truncated beta-galactosidase III from Bifidobacterium bifidum [Bifidobacterium bifidum]
6QUB_B	0.0	20	870	1	856	Truncatedbeta-galactosidase III from Bifidobacterium bifidum in complex with galactose [Bifidobacterium bifidum]
7NIT_A	0.0	6	1271	10	1290	ChainA, Beta-galactosidase [Bifidobacterium bifidum],7NIT_B Chain B, Beta-galactosidase [Bifidobacterium bifidum],7NIT_C Chain C, Beta-galactosidase [Bifidobacterium bifidum],7NIT_D Chain D, Beta-galactosidase [Bifidobacterium bifidum],7NIT_E Chain E, Beta-galactosidase [Bifidobacterium bifidum],7NIT_F Chain F, Beta-galactosidase [Bifidobacterium bifidum]
4YPJ_A	8.72e-188	20	860	1	800	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7LXS9	6.83e-152	17	865	33	846	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
T2KM09	6.63e-95	22	873	42	826	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	2.87e-44	29	776	54	780	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1
T2KN75	1.86e-42	21	901	21	810	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
P77989	3.39e-41	28	855	5	725	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000049	0.000001	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001267_00220.