CAZyme Information: MGYG000001275_01337

Basic Information

• Species: CAG-485 sp900321585
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485; CAG-485 sp900321585
• CAZyme ID: MGYG000001275_01337
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
664		73432.36	7.3147

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001275	2994513	MAG	Italy	Europe

• Gene Location: Start: 13581; End: 15575 Strand: -

Full Sequence      

MAAALSMALLAGSVSVLAAGPSAAAQGKDFAGYPYEPVPFTSVKVHDAFWGKRLDASRNV
TVPLAFSKCEESGRYANFVKAVHPSDTIKVEGLPFDDTDVYKTIEGASYLLQTYPDKKLE
AYIDSVLVLVAAAQEPDGYLNTLYTMNPKHPHPWMSPVKYEAVENLSHEFYNLGHMVEGA
VAHYRATGKRNFLDIAIKYADNICRNIGLGEGQLDRVPGHQIAEMALAKLYLVTGDKKYL
DQAKYFLDRRGKTSRHDLYSQSHKPVVDQKEAVGHAVRAVYMYSGMADVAALTGDSAYIK
AIDTIWNNMVTKKYYITGGIGARHQGEAFGDNYELPNSTAYCETCAAIGNVYVNHRLFLL
HGDSEYYDVLERTLYNGVLSGQSLNGDAFFYPNPLACDGSYSRKPWFGCACCPSNLSRFI
PSIPGYVYAVKDGDVYVNLYLANDGDLKVNGKRVRLSQTGNYVDDGEMAVTIDENKAGVF
TLKLRIPGWQLGRPVPGDLYSYTNPKAGNISVKVNGVEVPVENLQKGYLGINRKWKKGDK
VEIKFDNTPRMVVANELVKNNRGKVAIERGPIVYCAEWPDNDFDLAGVVIQKDAKISDGI
SQDSKLKGLHELSLPAQSISRNTDGKLGIKDVTLRMIPYYSWANRGEGRMRVWIPTELSS
INNE

Enzyme Prediction     

No EC number prediction in MGYG000001275_01337.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	43	575	1.7e-200	0.9980916030534351

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07944	Glyco_hydro_127	0.0	42	575	1	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG3533	COG3533	2.13e-173	37	654	11	585	Uncharacterized conserved protein, DUF1680 family [Function unknown].
cd04792	LanM-like	7.27e-05	170	307	545	681	Cyclases involved in the biosynthesis of class II lantibiotics, and similar proteins. LanM-like proteins. LanM is a bifunctional enzyme, involved in the synthesis of class II lantibiotics. It is responsible for both the dehydration and the cyclization of the precursor-peptide during lantibiotic synthesis. The C-terminal domain shows similarity to LanC, the cyclase component of the lan operon, but the N terminus seems to be unrelated to the dehydratase, LanB.
cd04791	LanC_SerThrkinase	2.19e-04	184	319	99	243	Lanthionine synthetase C-like domain associated with serine/threonine kinases. Some members of this subgroup lack the zinc binding site and the active site residues, and therefore are most likely inactive. The function of this domain is unknown.
COG1331	YyaL	7.34e-04	226	374	419	564	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QDO67740.1	0.0	32	660	27	653
QCD36182.1	0.0	14	660	10	654
ALJ59249.1	0.0	32	660	27	653
QUT89692.1	0.0	32	660	27	653
QUT86592.1	0.0	32	660	27	653

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6EX6_A	5.71e-319	32	662	4	631	TheGH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482],6EX6_B The GH127, Beta-arabinofuranosidase, BT3674 [Bacteroides thetaiotaomicron VPI-5482]
4QJY_A	2.26e-150	41	654	14	645	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	2.50e-146	41	654	14	645	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	7.89e-108	92	653	70	655	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	1.05e-107	92	653	70	655	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	4.32e-107	92	653	70	655	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000569	0.998484	0.000183	0.000281	0.000228	0.000210

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001275_01337.