CAZyme Information: MGYG000001275_02266

Basic Information

• Species: CAG-485 sp900321585
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Muribaculaceae; CAG-485; CAG-485 sp900321585
• CAZyme ID: MGYG000001275_02266
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
617		70221.01	6.2079

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001275	2994513	MAG	Italy	Europe

• Gene Location: Start: 10597; End: 12450 Strand: -

Full Sequence      

MLLVCGMAQAADEGTVLKLTSPDGVNEVTFSKPGRELTWQVRMNGADVILPSRAGLDLDN
RVWEMALGKRDLQQPQCWMDLLTVDSVRVHQPVDKTWHPQYGERSTVRDRYNSATMYMSR
HDKSKYRLNVEVRAYDEGIAFRYFFPEHPDAIFHKVTADLTDYSFPAGTMAWSEQWAQAR
FDRTGIDEIKQPVERALTLELPDGKWLAVTDADVDDWCLTKFVARDGKPGTLQSVMYSPV
DVVTYAATPWKIIMTADTPGQLLENNDIVLNLNPEAEGGFDWVKPGKIMRCTKLTTGAAM
EVIDFCAAHNIPYMLYDWKWYEPCTSHDGDATKVIPQIDMKKVVEYGREHGVGIWLYINQ
HALQKQHKELLPVVAGWGVKGVKSGFVQYASHRWATWLHDLVREAADNKLMMNIHDEFRP
SGFSRTYPNLVTQEGICGNEEFPDATHNVTLPFTRMINGAADYTICYYDKRLKNSHAHQL
AASLVFYSPMQTIFWYDNPRMYNGEPEIKWFEDLETTFDDTKVLGGFPGRDIVMARRKGD
IWWVAAMVNNDGAKETIDLSFLEPGRKYMAEIYTDGDDKVKTATKVAMSTRKVTSKDKLK
FDVPGRGGVAMRIVPVK

Enzyme Prediction     

No EC number prediction in MGYG000001275_02266.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	11	613	4.1e-160	0.9809825673534073

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14508	GH97_N	8.52e-59	21	274	1	234	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam10566	Glyco_hydro_97	9.89e-55	281	507	3	269	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14509	GH97_C	5.18e-37	518	614	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT92217.1	1.56e-317	1	616	9	631
ALJ62032.1	1.02e-316	1	616	9	631
QDO69864.1	1.19e-315	11	616	19	631
AND18133.1	6.36e-315	3	616	11	629
QUT85427.1	6.36e-315	3	616	11	629

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5E1Q_A	3.00e-55	13	613	13	654	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
3A24_A	4.32e-54	21	613	6	640	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
3WFA_A	2.50e-50	18	617	4	707	Catalyticrole of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron],3WFA_B Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron]
2JKA_A	2.53e-50	18	617	13	716	Nativestructure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKA_B Native structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKE_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKE_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKP_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482],2JKP_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482]
2D73_A	2.83e-50	18	617	24	727	CrystalStructure Analysis of SusB [Bacteroides thetaiotaomicron VPI-5482],2D73_B Crystal Structure Analysis of SusB [Bacteroides thetaiotaomicron VPI-5482],2ZQ0_A Crystal structure of SusB complexed with acarbose [Bacteroides thetaiotaomicron],2ZQ0_B Crystal structure of SusB complexed with acarbose [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	8.99e-54	19	613	25	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	1.55e-49	18	617	24	727	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
D7CFN7	5.34e-39	37	610	59	617	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.263888	0.732732	0.001124	0.001669	0.000298	0.000269

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001275_02266.