CAZyme Information: MGYG000001279_00939

Basic Information

• Species: Pseudomonas_E fulva
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas_E; Pseudomonas_E fulva
• CAZyme ID: MGYG000001279_00939
• CAZy Family: GT2
• CAZyme Description: Mannuronan synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
493	MGYG000001279_50|CGC1	56162.04	10.0444

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001279	4682858	MAG	Italy	Europe

• Gene Location: Start: 13489; End: 14970 Strand: -

Full Sequence      

MQRLKTALLQCAGWLFYMSLLMLIALALPADIFDPQSRHFIFLVGAVGIWRYSMGATHFI
RGMVFLYGVYPYLRRKVQKMGQAADPSHVYLMVTSFRIEALTTAQVYSSVIREAIDCGFP
TTVVCSLVEMSDELLVKSLWARYNPPPHVTLDIVRIAGTGKRDGLAYGFRAISRMLPDDD
AVVAVIDGDTVLDEGVVRKTVPWFKLFPNVGGLTTNEFCEVRGGYIMSEWHKLRFAQRHI
NMCSMALSKRVLTMTGRMSMFRASVVTNPEFIADVESDALMHWRLGRFKFLTGDDKSSWF
SLMRLGYDTFYVPDAAINTVEHPPEKSFFKASRKLMYRWYGNNLRQNSRALGLGLRRLGL
FTSIVLFDQRVSMWTSLLGLTVALIASLKFGMAYLLVYLLWIGFTRLVLTFMLLCSGHSV
GPAYPVILYYNQIVGALMKIYVFFRLDKQSWTRQPTTLKRDLASFQQWFNTWSSRAMTFS
AASLFVAALFMVV

Enzyme Prediction     

No EC number prediction in MGYG000001279_00939.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT2	86	342	8.8e-56	0.9956521739130435

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam13641	Glyco_tranf_2_3	3.64e-55	86	342	1	230	Glycosyltransferase like family 2. Members of this family of prokaryotic proteins include putative glucosyltransferase, which are involved in bacterial capsule biosynthesis.
pfam13632	Glyco_trans_2_3	7.75e-22	182	394	1	194	Glycosyl transferase family group 2. Members of this family of prokaryotic proteins include putative glucosyltransferases, which are involved in bacterial capsule biosynthesis.
cd06434	GT2_HAS	4.05e-11	160	345	64	234	Hyaluronan synthases catalyze polymerization of hyaluronan. Hyaluronan synthases (HASs) are bi-functional glycosyltransferases that catalyze polymerization of hyaluronan. HASs transfer both GlcUA and GlcNAc in beta-(1,3) and beta-(1,4) linkages, respectively to the hyaluronan chain using UDP-GlcNAc and UDP-GlcUA as substrates. HA is made as a free glycan, not attached to a protein or lipid. HASs do not need a primer for HA synthesis; they initiate HA biosynthesis de novo with only UDP-GlcNAc, UDP-GlcUA, and Mg2+. Hyaluronan (HA) is a linear heteropolysaccharide composed of (1-3)-linked beta-D-GlcUA-beta-D-GlcNAc disaccharide repeats. It can be found in vertebrates and a few microbes and is typically on the cell surface or in the extracellular space, but is also found inside mammalian cells. Hyaluronan has several physiochemical and biological functions such as space filling, lubrication, and providing a hydrated matrix through which cells can migrate.
COG1215	BcsA	6.40e-06	41	411	9	402	Glycosyltransferase, catalytic subunit of cellulose synthase and poly-beta-1,6-N-acetylglucosamine synthase [Cell motility].
cd06436	GlcNAc-1-P_transferase	1.79e-04	96	266	1	175	N-acetyl-glucosamine transferase is involved in the synthesis of Poly-beta-1,6-N-acetyl-D-glucosamine. N-acetyl-glucosamine transferase is responsible for the synthesis of bacteria Poly-beta-1,6-N-acetyl-D-glucosamine (PGA). Poly-beta-1,6-N-acetyl-D-glucosamine is a homopolymer that serves as an adhesion for the maintenance of biofilm structural stability in diverse eubacteria. N-acetyl-glucosamine transferase is the product of gene pgaC. Genetic analysis indicated that all four genes of the pgaABCD locus were required for the PGA production, pgaC being a glycosyltransferase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CRN05282.1	0.0	1	493	1	493
QDC05853.1	0.0	1	493	4	496
AVF54375.1	0.0	1	493	4	496
QPH49987.1	0.0	1	493	1	493
QPH44913.1	0.0	1	493	1	493

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q88NC5	0.0	1	493	4	496	Glycosyltransferase alg8 OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=alg8 PE=3 SV=1
Q887P9	1.67e-304	1	489	1	489	Glycosyltransferase alg8 OS=Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000) OX=223283 GN=alg8 PE=3 SV=1
Q52463	7.93e-294	1	493	2	494	Mannuronan synthase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=alg8 PE=1 SV=2
P94199	1.66e-255	1	489	1	488	Glycosyltransferase alg8 OS=Azotobacter vinelandii OX=354 GN=alg8 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000028	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
13	33
48	70
380	402
422	444