CAZyme Information: MGYG000001293_00051

Basic Information

• Species: Lacticaseibacillus rhamnosus
• Lineage: Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lacticaseibacillus; Lacticaseibacillus rhamnosus
• CAZyme ID: MGYG000001293_00051
• CAZy Family: GH59
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1492		160712.35	5.4437

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001293	3010111	Isolate	not provided	not provided

• Gene Location: Start: 52070; End: 56548 Strand: +

Full Sequence      

MKAKLIGSKPKSSRYRLLWSGIVMAMMAVSLVILLTIPAATRAASQNPEDTVAVHLDGND
IASNNTNGLTFKGFGVLSGNGTSALLMDYKSEQPDVYAKMMKTLFGGSQPIMTNLKLEMG
NDRNNSTGSEPATQRTESEAANVTRDQGFQLVADAKKINPNLKVTILRWNAPGWAKTNDQ
IYTWYKNSILNAYRTYGYMVDYVNPGLNEHKPDLAWAKEYAERVKTDNTGFKNDTERQLY
NKIKVVISDEVGVGSFGKDMVTDSSLRNAVTAAGYHYSTEDDSAKNFTALADKYDKEVWN
SEAQATFGNSDYRPNSGTGIGVAGSSLEMANTIVKGFTDSRRTNFIYQPAIAAFYEGGQY
SSKSVLQATDPWSGWTNSDVAIDVLAQFSKFAKLSWENSDNTSGIWRAVSQASVSTATGS
NNVNGRNGLANYLTLASPDKKDFSTVIVNDSKYTKHYQISASNMAYKGTPTLEEWETRAA
DTTAKDAYDSNYLKHVGDVQADSKGVYTVTVKPFSIKTVTTLDKAKDSDLNQGISASSNK
QRTVLDTDANGKGTDTSDTTLYADNFEYGGKKVATLNADGTSNTAKTEDFITSRGGDEGF
YPLYTFNRNGTLEGYKTTNAKSGHYVLRQQLDSTVVEPGGAWNDGDALAWIGDNRWMNYK
ASTDVSFEDKGTHGSANYASIGARQQADSGPAAYLKFWQDGGWSLHIGSQSVASGNVATG
QGGTKISGFDTTNTAWHNIAIQAAGNTITASIDGQKVADGKVTSELSGRVTLGSGYFHTD
FDNLKVETVKGYTPYYSQQIDDLEMYDTSATPKQQLVYNDQWTHETEQGMYLRDRTVSKN
TGAGATLTYTFTGTGLDICGNNDGSVKLDVTVDGKQVATDAATNKADNLGQTYTLRNLKY
GQHTVTFTVKSGTLAVDYVGVVPSDTIADFSKLQTAYDKVKDVTNADNKYTSSSWTSFQK
VLLAAKNVLADTTVSQNDIDTALKNLNTAYAALALSPDKTKLQTAYDKAKAVTNPSDKYT
AASCKVFQTALTDAQYVLANANATQNDVDSALKNLNQAASDLVLNPAKPDKTKLQAAYDK
NKAVTNPDDKYTSESWQAFQTALSDAQKVLTDTNATQNDVDNALKSLNQAATDLTLNPAK
PDKTKLQAAYDKNKAVTNPNDKYTSDSWQAFQTALSDAQKVLANTNATQDSLDTTLQKLN
NAYAGLKLNSQKPDKSALQTAYDKDSTVTNQANKYTTASWTTFQSALANAKKVLADTDAV
QADVDTALQKLNDAYDGLTLSPDKSALQTALTEAQTLSHSAVTGDHEGNYPADALKTLQV
AIDTAKKVADNPDASKDDIDTAASTLKQAVTAFKQTVVTVSRDQLTQLVTESQTLRADDY
TQDSWTPYQQAVAAAQKLLAGKPSQQELDAAATALKKAKTSLVAAPTGKLPSTGDASSES
ASSSEETASSSTAKYPSTGEAQLSLAVTMAAVVFLIGISGFAWFLHQKGKAK

Enzyme Prediction     

EC	3.2.1.23

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH59	65	788	5.1e-170	0.9952456418383518

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam02057	Glyco_hydro_59	3.31e-95	71	389	1	292	Glycosyl hydrolase family 59.
pfam17996	CE2_N	1.15e-05	835	911	4	84	Carbohydrate esterase 2 N-terminal. This is the N-terminal beta-sheet domain with jelly roll topology found in CE2 acetyl-esterase from the bacterium Clostridium thermocellum. This enzyme displays dual activities, it catalyses the deacetylation of plant polysaccharides and also potentiates the activity of its appended cellulase catalytic module through its noncatalytic cellulose binding function. This N-terminal jelly-roll domain appears to extend the substrate/cellulose binding cleft of the catalytic domain in C.thermocellum.
pfam07554	FIVAR	1.77e-04	1072	1135	1	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
pfam07554	FIVAR	8.53e-04	1216	1279	1	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
COG5520	XynC	0.003	156	347	110	306	O-Glycosyl hydrolase [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AQY36041.1	0.0	1	1492	1	1492
BAI40578.1	0.0	1	1492	1	1492
CAR85946.1	0.0	1	1492	1	1492
QFG47696.1	0.0	1	1492	1	1492
AZZ21772.1	0.0	1	1492	1	1492

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4CCC_A	4.93e-17	68	668	22	538	StructureOf Mouse Galactocerebrosidase With 4nbdg: Enzyme-substrate Complex [Mus musculus],4CCD_A Structure Of Mouse Galactocerebrosidase With D-galactal: Enzyme-intermediate Complex [Mus musculus],4CCE_A Structure Of Mouse Galactocerebrosidase With Galactose: Enzyme-product Complex [Mus musculus],4UFH_A Mouse Galactocerebrosidase complexed with iso-galacto-fagomine IGF [Mus musculus],4UFI_A Mouse Galactocerebrosidase complexed with aza-galacto-fagomine AGF [Mus musculus],4UFJ_A Mouse Galactocerebrosidase complexed with iso-galacto-fagomine lactam IGL [Mus musculus],4UFK_A Mouse Galactocerebrosidase complexed with dideoxy-imino-lyxitol DIL [Mus musculus],4UFL_A Mouse Galactocerebrosidase complexed with deoxy-galacto-noeurostegine DGN [Mus musculus],4UFM_A Mouse Galactocerebrosidase complexed with 1-deoxy-galacto-nojirimycin DGJ [Mus musculus],5NXB_A Mouse galactocerebrosidase in complex with saposin A [Mus musculus],5NXB_B Mouse galactocerebrosidase in complex with saposin A [Mus musculus],6Y6S_A Chain A, Galactocerebrosidase [Mus musculus],6Y6T_A Chain A, Galactocerebrosidase [Mus musculus]
3ZR5_A	4.95e-17	68	668	24	540	STRUCTUREOF GALACTOCEREBROSIDASE FROM MOUSE [Mus musculus],3ZR6_A STRUCTURE OF GALACTOCEREBROSIDASE FROM MOUSE IN COMPLEX WITH GALACTOSE [Mus musculus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q5SNX7	4.34e-21	68	786	30	657	Galactocerebrosidase OS=Danio rerio OX=7955 GN=galc PE=2 SV=1
B5X3C1	7.69e-21	68	787	34	663	Galactocerebrosidase OS=Salmo salar OX=8030 GN=galc PE=2 SV=1
O02791	6.73e-19	68	522	52	469	Galactocerebrosidase OS=Macaca mulatta OX=9544 GN=GALC PE=1 SV=2
P54803	1.54e-18	68	522	52	469	Galactocerebrosidase OS=Homo sapiens OX=9606 GN=GALC PE=1 SV=3
P54804	1.22e-16	68	522	36	453	Galactocerebrosidase OS=Canis lupus familiaris OX=9615 GN=GALC PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.047156	0.951120	0.000597	0.000533	0.000274	0.000267

TMHMM  Annotations     

start	end
17	39
1463	1485