CAZyme Information: MGYG000001299_00386

Basic Information

• Species: Amedibacillus dolichus
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelotrichaceae; Amedibacillus; Amedibacillus dolichus
• CAZyme ID: MGYG000001299_00386
• CAZy Family: GH31
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2314		253016.67	4.5672

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001299	2190453	Isolate	not provided	not provided

• Gene Location: Start: 87797; End: 94741 Strand: +

Full Sequence      

MFKKVVASGLSLMMVLSSTGYQQLVQVHAETNMLSKQLSGIKSVEADKSEKNVVWVTFTN
GYKGKLTFLDNGIFRYNVDPKGEFSEYATPRSSAHKARIQQYPDDSDKYKHPEATVTNQG
GVYTISAGDVTVTFNQQALMSVKSGNKVVLQETSPLSIGDRSVVQTLAKNDNENYYGGGT
QNGRFVHTGKTINIANESGWNDGQVSSPNPFYWSTAGYGVLRNTFADGKYDFGETNSETA
TATHDEGEFDAYYFVSDGANGAEVSQDILQEYFKVTGNPALLPEYGFYEGHLNCYNRDAW
ADGEQKADPSKGNKTWVTTNPDGTEFKQTEAGGTGYEIKENLQSESLMGKGPTTKNNVPS
GVTTPEQFSAKAVLDRYVENDMPLGYFLPNDGYGCGYGQNGYKVTGGVEADGSSNAERTA
AIDANVANLKEFTDYANSKGVDTGLWTQSQLSPDSNNNTSWHLLRDFKKEVKNGGITTLK
TDVAWVGQGYSMQLDGVKTAYDTITTEVNKRPNIISLDGWAGSQRFNSVWTGDQAGGNWE
YIRFHIPTYIGQSLSGNPNIGSDMDGIHGGAPLIATRDYQWKTFTPQMLNMDGWGTYTKA
PHAFADPYKSTSRMYLKLKGQLLPYIYTSAASASNLNTKNGDTGLPMIRAMFLEFPNDDY
AYSKNMQYQYMFGDAFLVAPVYQNTELNEETGADIRNDIYLPKGETWIDYFTGEQYRGGT
VINNFDAPLWKLPLFVKNGSIVPMYEENNSPDKINRANRIVEFWPEGESTYTAYEDDGKS
ITNKTEKDEDYGVIDNISYGNHSELTYKSVVNEEKKQATLTAEKVNVTGSYDGYSAEKNT
TFVVNVSKKPTKLTAKNGSSTLKNVEVNSKAEFDAKTPNAGEAVFFYDANPTIETYANEH
ETKIKEMVKDVKVAPKLYVKFAKTNATENAQQLVIDGFVNDGNLPNDGENASLDAPKNIE
YKPSSDSIALTWDEVEGATSYDVEADGIVYSMLEKPAYTHSGLNYLEKHTYRVRSVNKDG
HSEWSELKEVSTLDDPYRNVPKGMTVSYDQGNTPAAYAGKFEYMVDGDDVTEYSSADAGV
FNGKSFTIDMKKIYSINKLDYVFRQDGSNGTIKKFKLSYSNDGVNWKKYKDGEEINLTPT
TQWPLGDKNKLNTQNVNARYATVDLEQFKARYIKVELVETAGGFLQAYEIRPYHVDKDKG
VMPGDFEANGIINDNDEVWLDSHNPEAAGARKGGAFYESDANKFDLNENGIFDAYDIAVL
TTQLNGGVKEKTNASGVLSAVPSKQNVKAGETFTVDIYGSGLKNVYAFDFELSFDKGALS
YSDDDFVVKTTAKTRNQKVYEYLHHEETDEKDRVYATITNLGNQTSLNGDMKLATVTLKA
NKELDLSNLQLSHGLVVGSSLNYVNGLDPVKPNEPSDMVEKRLVAHEDIVKAKAKSNGPV
GDGNDIWASLDQNNSTYTNSNYGNAANAHPQVYTFELKDKMSLSKVGVRPRSANHYGQLG
SFKVFVSDDKNTWTPVSDKVTVNATTADSEGYTSVELPKGTSAKYVKLELEPATADGNCV
ATSEVAIYSAASVKPTSIVFEKDTMELHIGHLTPVKAIVAPEDAANKLYEVTSDNSKVKV
ITSSDANGYYYSLLALEKLNDGEVVTLTATSKADPKLKDTMTVTVTDKAFVDDLNAAIAE
AEKVVNEKDLYTSASVDVVEKALAAAKEAVKNGDQATVDKAEIDLLNAIAGLEFKGSDDT
RPDSTNHIDEGQMEVISVTNYADSDIKDHIIDNNADSIWHSSYGQGAKLPVDAVIDLGAV
YQLEQVDYLPRQSSSNGHITHYRIEVSTDNKTFTPVVEGYLPNNGYELDNKEVAKMIKFA
PVEAQYVKFIALGTIGDSGKNNDRYASIAELDFYGTTNAAVTNVAFENDAMEMVIAEQKQ
LKAITTPSNSTEQLVWSSDKEDVVSVDQNGVATAKAAGTATITVKKATDANISASMTITV
KALDKSVLEAKIKEVSEFKATVKDEKVVAYLTEEIAKAQAVVDNAGATESDVTQAVEALT
SAKDTAVKAIDVLNRFAAVKALDLSKFDPAGQEELLALISEVEELAKEPITNLDVLTEKV
AKLEELSGKLLPLDVTTLENLIKVAEGIELDKYVDNDAKDAFSAALNKAKEVAANPKTLA
EIKAAETALKDAEGKLVLKATTATKDALKDLYDEFAKLDLENYSNKHQEQIRDMMDRIQS
ALKDEQLSEDDAKALLSEAAKVYAELPAISPLPDPNNPGNGQNPDGNNGAGQKPNGDSTN
TGDTTNAALYSMLLLASAGGVALIGMKKKKANKE

Enzyme Prediction     

No EC number prediction in MGYG000001299_00386.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH31	266	742	1.1e-61	0.9765807962529274
CBM32	1761	1891	5.5e-19	0.8870967741935484
CBM32	1055	1189	1e-15	0.8709677419354839

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd06596	GH31_CPE1046	1.24e-153	275	713	1	334	Clostridium CPE1046-like. CPE1046 is an uncharacterized Clostridium perfringens protein with a glycosyl hydrolase family 31 (GH31) domain. The domain architecture of CPE1046 and its orthologs includes a C-terminal fibronectin type 3 (FN3) domain and a coagulation factor 5/8 type C domain in addition to the GH31 domain. Enzymes of the GH31 family possess a wide range of different hydrolytic activities including alpha-glucosidase (glucoamylase and sucrase-isomaltase), alpha-xylosidase, 6-alpha-glucosyltransferase, 3-alpha-isomaltosyltransferase and alpha-1,4-glucan lyase. All GH31 enzymes cleave a terminal carbohydrate moiety from a substrate that varies considerably in size, depending on the enzyme, and may be either a starch or a glycoprotein.
COG1501	YicI	1.16e-66	55	825	56	753	Alpha-glucosidase, glycosyl hydrolase family GH31 [Carbohydrate transport and metabolism].
pfam01055	Glyco_hydro_31	3.71e-56	430	742	159	442	Glycosyl hydrolases family 31. Glycosyl hydrolases are key enzymes of carbohydrate metabolism. Family 31 comprises of enzymes that are, or similar to, alpha- galactosidases.
cd06603	GH31_GANC_GANAB_alpha	4.65e-43	509	780	211	467	neutral alpha-glucosidase C, neutral alpha-glucosidase AB. This subgroup includes the closely related glycosyl hydrolase family 31 (GH31) isozymes, neutral alpha-glucosidase C (GANC) and the alpha subunit of heterodimeric neutral alpha-glucosidase AB (GANAB). Initially distinguished on the basis of differences in electrophoretic mobility in starch gel, GANC and GANAB have been shown to have other differences, including those of substrate specificity. GANC and GANAB are key enzymes in glycogen metabolism that hydrolyze terminal, non-reducing 1,4-linked alpha-D-glucose residues from glycogen in the endoplasmic reticulum. The GANC/GANAB family includes the alpha-glucosidase II (ModA) from Dictyostelium discoideum as well as the alpha-glucosidase II (GLS2, or ROT2 - Reversal of TOR2 lethality protein 2) from Saccharomyces cerevisiae.
cd08759	Type_III_cohesin_like	2.89e-40	1276	1455	1	167	Cohesin domain, interaction partner of dockerin. Bacterial cohesin domains bind to a complementary protein domain named dockerin, and this interaction is required for the formation of the cellulosome, a cellulose-degrading complex. Two specific calcium-dependent interactions between cohesin and dockerin appear to be essential for cellulosome assembly, type I and type II. This subfamily represents type III cohesins and closely related domains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBK61154.1	0.0	1	1921	1	1947
BBK23937.1	0.0	50	2314	67	2280
QJA02122.1	0.0	41	2310	60	2301
BCT46261.1	0.0	24	2091	31	2073
QWT17625.1	0.0	35	2044	47	2097

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6M76_A	2.38e-249	44	1038	41	963	GH31alpha-N-acetylgalactosaminidase from Enterococcus faecalis [Enterococcus faecalis ATCC 10100],6M77_A GH31 alpha-N-acetylgalactosaminidase from Enterococcus faecalis in complex with N-acetylgalactosamine [Enterococcus faecalis ATCC 10100]
7F7R_A	1.26e-248	44	1038	41	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
7F7Q_A	3.44e-248	44	1038	41	963	ChainA, GH31 alpha-N-acetylgalactosaminidase [Enterococcus faecalis ATCC 10100]
4LKS_A	7.09e-33	1748	1895	21	166	Structureof CBM32-3 from a family 31 glycoside hydrolase from Clostridium perfringens in complex with galactose [Clostridium perfringens ATCC 13124],4LKS_C Structure of CBM32-3 from a family 31 glycoside hydrolase from Clostridium perfringens in complex with galactose [Clostridium perfringens ATCC 13124],4LQR_A Structure of CBM32-3 from a family 31 glycoside hydrolase from Clostridium perfringens [Clostridium perfringens ATCC 13124],4P5Y_A Structure of CBM32-3 from a family 31 glycoside hydrolase from Clostridium perfringens in complex with N-acetylgalactosamine [Clostridium perfringens ATCC 13124]
4LPL_A	4.80e-27	1016	1193	22	181	Structureof CBM32-1 from a family 31 glycoside hydrolase from Clostridium perfringens [Clostridium perfringens ATCC 13124]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9F234	3.27e-32	510	803	461	734	Alpha-glucosidase 2 OS=Bacillus thermoamyloliquefaciens OX=1425 PE=3 SV=1
Q9P999	1.44e-28	486	791	376	669	Alpha-xylosidase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=xylS PE=1 SV=1
Q9FN05	9.00e-25	373	781	436	821	Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1
B9F676	2.67e-24	247	780	328	818	Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1
P38138	2.51e-21	495	781	580	851	Glucosidase 2 subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ROT2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001371	0.992454	0.005307	0.000357	0.000258	0.000212

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001299_00386.