CAZyme Information: MGYG000001304_00124

Basic Information

• Species: Erysipelatoclostridium spiroforme
• Lineage: Bacteria; Firmicutes; Bacilli; Erysipelotrichales; Erysipelatoclostridiaceae; Erysipelatoclostridium; Erysipelatoclostridium spiroforme
• CAZyme ID: MGYG000001304_00124
• CAZy Family: CBM32
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2006	MGYG000001304_1|CGC4	226644.26	4.1765

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001304	2507485	Isolate	not provided	North America

• Gene Location: Start: 156090; End: 162110 Strand: +

Full Sequence      

MKKIGKIFLSTLLCSSMLVVPNYKVMAQENGRITNISASGKSSSNHEANLAIDGNKDTYY
MTPASNTMQDHYRNIDLNLDGIYELSKIVIYNNEGSYNHYQIYTSSDGVNYDKVAYKSDD
KMASDTGDEYTLDVNASHVRINLSYNSNQMEGNLAEVELYGSKVGELNDKIEKIEVADFK
DTKWVKEYEKFSTDENYANDKTIKEMSALVGRVIGEKWQDSFVFEIREANDGKDVFEIEN
GENGKIVIRGNDGVSMASAFNYYLRYYCNVDYNPLFASQLKMPETLPQLNSKIVKETQYD
YRYALNFCTYSYTMAFWNWDEYEAFLDWAAMSGINTMLDIVGQEEVIRRTLSAYGYSDEE
IKEYIAGPGYFAWFYMQNMTSYGGKLPNNWFEERVELARKMHDRMQTYGITPVLSGFSGQ
VPTNFKDKYQDVQYVAQGSWCGYERPDMLRTYVDNGGTDYFSQMADVFYKAQRDIFGDVT
NIYAVDPFHEGGKIGDMNYTKVYETVQKKMMENDEDAIWLIQEWSGSIASNPSKLINLDK
EHVIVLDLFSEVSPRNSALEAADTPWIWNMLHNFGGRMGLDANPEKVSQNIPNTYQNSEH
MVGIGMTPEAIENSPMAYELLWDMTWTKDPIDFRQWCQDYAKRIYGGTNEDIEEVWNILL
DTGYNRKDNYYQGAPESVINARPTTNFTSASSWGHSTINYDKEELERAVYLMAKNYDEFK
DSPAFIYDLSDITRQLISNSAQEYHKAMVNAYQAGNLSEFEVLSDKFLEMILLQDQILST
NSDFLVGKWIEQARTMIEDSDDWTKDLFEFNARDLITTWGGLKNANGGGLRDYSNRQWAG
LTKDYYYPRWQKWINDVKEAMKNNTAVPSTDWFLMEWEWANLKSDEGNEYSIEASNLALD
ELAMKAYNEYSISSLENLVGNVEEKENIALGKKVSASINNVPTNPTDNLTDGNKETTWIG
ESNVSSFDLTVDLEAESSIDGMEISLKQIAGGFPYTYKVEVFSENQWNVVAQNDDGEITS
QTLIDYKGIASKVRFTFNTTDTSIIPEVAELAIYGKVEKQPNYKNIAVGAPVTTPEGTTT
KITDDDPSTLWVRNGDKYPALLTLDLGKEEYVDVLELYFEKPGLRYQYDVVIEDGNGNKT
TLQDKSDNTEDLAGMYRLPVNQKVQKVYVNLMARAPGGEFYLAWPALAEIKLLQETEIEF
EYQNIAYGKPGHVINQNSYDSSKLTDGSTTGLENVGHDDFPTTFQVDLGSEQFIEEVKIY
FEKPGIRFKFKVEVEDLAGNKTVILDKTDNVDDLDASYTIDAKVNGAKVNVVIEGRAPGG
EFYLASPAMTEIEVFAKPETITNDSKVSGNMSLTDEQKSALIDNDNATSVEFNSNEEKEL
IFEFDKLVDIYAYELYKLSDEALQYKVEYLSSDNNWEVLSDESNNLDNSSKYVGEFDAVL
TNKVCLTIFNNSCNISGFNVYRYDPTIELESYIGNIENKVSKVEIGEYAGNYSLEAKQVL
DDKISKTRELIDSDINSLEVKQQQEELNKAYNEFLRSYISVDRTALLVELTEVNELLKLD
SLKDNVLLNEAYVNAKNVYDTYKVTQVELDNSTKLLKEAKDEALLLISKQEIYQEQLAIA
KELIETTEIGEYNGNVSQETMDVFKNTIDNIEKGYADAITADDVEEIINSLKEAIETFND
NIIVVDKTELKAVIDDATNLNENDYTKESWNAMQEVLMEAKTVLETEKVTNKQVEDAKNN
LLKAIDDLVKVKVDKTALKIAVDLANALTDEDLANVVPVVANEFKAARDEANAVYNNVSA
TQDEVNNAFDRLASAMQKLEFFKGDKKALKAFIDDVTGLDATKYTQTTWTAFNDALTAAN
DVYGDVNAMQEEVNEAYTNLVTAFLNLRLISDKSLLEELINQANGLNVANYTKESFDGLT
KALDEAKVVFDNPDATQKEVDSAKDVLAKALAGLQTVTTDNTVKTPVNKSDTTVSVKTGD
ESLAGMLAGLALLSVAGYAVLRRKED

Enzyme Prediction     

No EC number prediction in MGYG000001304_00124.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH89	243	887	1.7e-228	0.971342383107089
CBM32	38	157	7.4e-16	0.9274193548387096

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam05089	NAGLU	6.32e-134	302	628	1	333	Alpha-N-acetylglucosaminidase (NAGLU) tim-barrel domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This central domain has a tim barrel fold.
pfam12972	NAGLU_C	2.84e-89	636	895	1	250	Alpha-N-acetylglucosaminidase (NAGLU) C-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This C-terminal domain has an all alpha helical fold.
pfam12971	NAGLU_N	4.99e-28	204	287	1	81	Alpha-N-acetylglucosaminidase (NAGLU) N-terminal domain. Alpha-N-acetylglucosaminidase, a lysosomal enzyme required for the stepwise degradation of heparan sulfate. Mutations on the alpha-N-acetylglucosaminidase (NAGLU) gene can lead to Mucopolysaccharidosis type IIIB (MPS IIIB; or Sanfilippo syndrome type B) characterized by neurological dysfunction but relatively mild somatic manifestations. The structure shows that the enzyme is composed of three domains. This N-terminal domain has an alpha-beta fold.
pfam00754	F5_F8_type_C	6.24e-10	35	157	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	5.72e-07	933	1051	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYE33274.1	0.0	23	1954	27	2131
QAS61445.1	0.0	23	1954	27	2131
ABG84150.1	0.0	24	1744	20	1920
AMN35090.1	0.0	24	1744	29	1929
AMN32327.1	0.0	24	1744	29	1929

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VC9_A	0.0	36	923	10	890	Family89 Glycoside Hydrolase from Clostridium perfringens in complex with 2-acetamido-1,2-dideoxynojirmycin [Clostridium perfringens],2VCA_A Family 89 glycoside hydrolase from Clostridium perfringens in complex with beta-N-acetyl-D-glucosamine [Clostridium perfringens],2VCB_A Family 89 Glycoside Hydrolase from Clostridium perfringens in complex with PUGNAc [Clostridium perfringens],2VCC_A Family 89 Glycoside Hydrolase from Clostridium perfringens [Clostridium perfringens]
4A4A_A	0.0	36	923	33	913	CpGH89(E483Q, E601Q), from Clostridium perfringens, in complex with its substrate GlcNAc-alpha-1,4-galactose [Clostridium perfringens]
7MFK_A	0.0	36	923	18	898	ChainA, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124],7MFL_A Chain A, Alpha-N-acetylglucosaminidase family protein [Clostridium perfringens ATCC 13124]
4XWH_A	3.93e-90	208	861	11	663	Crystalstructure of the human N-acetyl-alpha-glucosaminidase [Homo sapiens]
4A6O_A	8.93e-28	1062	1193	25	160	CpGH89CBM32-4,produced by Clostridium perfringens, in complex with glcNAc-alpha-1,4-galactose [Clostridium perfringens],4A6O_B CpGH89CBM32-4, produced by Clostridium perfringens, in complex with glcNAc-alpha-1,4-galactose [Clostridium perfringens]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q9FNA3	5.03e-99	224	899	64	794	Alpha-N-acetylglucosaminidase OS=Arabidopsis thaliana OX=3702 GN=NAGLU PE=2 SV=1
P54802	2.01e-89	208	861	34	686	Alpha-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=NAGLU PE=1 SV=2
E8MGH9	1.86e-12	1753	2006	1666	1933	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1
P26831	5.80e-09	1703	1952	1321	1566	Hyaluronoglucosaminidase OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=nagH PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000291	0.998939	0.000266	0.000173	0.000161	0.000139

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001304_00124.