CAZyme Information: MGYG000001307_00442

Basic Information

• Species: Providencia stuartii
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Providencia; Providencia stuartii
• CAZyme ID: MGYG000001307_00442
• CAZy Family: GH15
• CAZyme Description: Glucoamylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
769	MGYG000001307_5|CGC1	84953.71	5.5115

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001307	4603561	Isolate	not provided	not provided

• Gene Location: Start: 78068; End: 80377 Strand: +

Full Sequence      

MIAQQAPGAPGSTATWTSSAKDMVGTAIGQGRVWFTVGYGILNEVYWPSCSTPQIRDLGF
IIAGDDFWSEVKRVHQYSITTPSSSLPLPKIVHQHERYRLELEIITDPARDVLLIHYHLE
GEGLRLYPLLAPHLGGDGDNNYGSVTSQGLAAQKNGQYLILSAENGFARGSVGYVGSSDG
WQDFSQNGGMSWEYQQAGPGNIAMIGELNHNSGVLALAFSASAQGAATLANSALAEGYQE
ARRQYAYLWAEWNETVNFPGLDKLPKTLSDAVRTSVVVLKTHEGRTFPGSLVASLSTPWG
DTHKDPGGYHLVWPRDSVEVGFAMLACGLITEVRALLAYLIAIQQPDGHWMQNNFTDGRP
YWQGIQLDEVALPVLFAAKLHEQGLLGEMQGAAIRMARKALAFIAQYGPASPQDRWEENS
GINPFTLSVSIAALVAGAKAGFLEDGDKQYALDLADDWNERLESWVYVANTELDKELGIS
GHYIRLNPNCHSARFGEVMLRNRNNETINTRALLGMEYLYLVRLGLRKASDKRIEETTKL
VDEQLCVNLPAGPYYYRYNEDGYGEHEDGRAFDGSGVGRLWPLLSGERGHYAAARKEDIK
PYLNAILASASTGGMLPEQIWDKESIPERGLLTGRPSGSAMPLIWAHAELIKLIYVQKTG
VPIEQLKSVSERYGNKPPAPRARHWRDNLSCGAIPANRQLWIEAQEPFVLHYGYDNWQDI
KEQSSQPLGLGLYGVALDVSALAGKTLQFTRRFDAKGWEGRDWQVEIEV

Enzyme Prediction     

No EC number prediction in MGYG000001307_00442.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH15	279	654	6.4e-79	0.9667590027700831

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR01535	glucan_glucosid	0.0	32	673	2	648	glucan 1,4-alpha-glucosidase. Glucan 1,4-alpha-glucosidase catalyzes the hydrolysis of terminal 1,4-linked alpha-D-glucose residues from non-reducing ends of polysaccharides, releasing a beta-D-glucose monomer. Some forms of this enzyme can hydrolyze terminal 1,6- and 1,3-alpha-D-glucosidic bonds in polysaccharides as well. [Energy metabolism, Biosynthesis and degradation of polysaccharides]
cd07430	GH15_N	3.54e-109	7	252	1	260	Glycoside hydrolase family 15, N-terminal domain. Members of this family are N-terminal domains uniquely found in bacterial and archaeal glucoamylases and glucodextranases. Glucoamylase (glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; amyloglucosidase; exo-1,4-alpha-glucosidase; gamma-amylase; lysosomal alpha-glucosidase; EC 3.2.1.3) hydrolyzes beta-1,4-glucosidic linkages of starch, glycogen and malto-oligosaccharides, releasing beta-D-glucose from the non-reducing end. Glucodextranase (glucan 1,6-alpha-glucosidase; exo-1,6-alpha-glucosidase; EC 3.2.1.70) uses an inverting reaction mechanism to hydrolyze alpha-1,6-glucosidic linkages of dextran and related oligosaccharides, releasing beta-D-glucose from the non-reducing end. These N-terminal domains adopt a structure consisting of antiparallel beta-strands, divided into two beta-sheets, with one sheet wrapped by an extended polypeptide, which appears to stabilize the domain. The function of these domains in the enzymes is as yet unknown. However, it is suggested that domain N of bacterial GA is involved in folding and/or the thermostability of the A domain that forms an (alpha/alpha)6-barrel structure.
pfam09137	Glucodextran_N	3.41e-96	6	252	1	261	Glucodextranase, domain N. Members of this family, which are uniquely found in bacterial and archaeal glucoamylases and glucodextranases, adopt a structure consisting of 17 antiparallel beta-strands. These beta-strands are divided into two beta-sheets, and one of the beta-sheets is wrapped by an extended polypeptide, which appears to stabilize the domain. Members of this family are mainly concerned with catalytic activity, hydrolysing alpha-1,6-glucosidic linkages of dextran to release beta-D-glucose from the non-reducing end via an inverting reaction mechanism.
COG3387	SGA1	1.37e-69	33	655	9	602	Glucoamylase (glucan-1,4-alpha-glucosidase), GH15 family [Carbohydrate transport and metabolism].
pfam00723	Glyco_hydro_15	6.41e-28	270	655	5	417	Glycosyl hydrolases family 15. In higher organisms this family is represented by phosphorylase kinase subunits.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVE41515.1	0.0	1	769	1	769
BBV06664.1	0.0	1	769	1	769
QET98155.1	0.0	1	769	1	769
QIB28400.1	0.0	1	769	1	769
QPN40736.1	0.0	1	769	1	769

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1UG9_A	2.06e-116	7	678	6	688	CrystalStructure of Glucodextranase from Arthrobacter globiformis I42 [Arthrobacter globiformis],1ULV_A Crystal Structure of Glucodextranase Complexed with Acarbose [Arthrobacter globiformis]
1LF6_A	1.11e-102	10	653	26	662	CRYSTALSTRUCTURE OF BACTERIAL GLUCOAMYLASE [Thermoanaerobacterium thermosaccharolyticum],1LF6_B CRYSTAL STRUCTURE OF BACTERIAL GLUCOAMYLASE [Thermoanaerobacterium thermosaccharolyticum],1LF9_A Crystal Structure Of Bacterial Glucoamylase Complexed With Acarbose [Thermoanaerobacterium thermosaccharolyticum],1LF9_B Crystal Structure Of Bacterial Glucoamylase Complexed With Acarbose [Thermoanaerobacterium thermosaccharolyticum]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P29761	4.00e-99	10	653	43	679	Glucoamylase OS=Clostridium sp. (strain G0005) OX=72582 GN=cga PE=1 SV=1
Q59005	1.38e-11	309	655	296	607	Uncharacterized glycosyl hydrolase MJ1610 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ1610 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999747	0.000292	0.000001	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001307_00442.