CAZyme Information: MGYG000001311_00504

Basic Information

• Species: Enterocloster sp000155435
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Enterocloster; Enterocloster sp000155435
• CAZyme ID: MGYG000001311_00504
• CAZy Family: GH127
• CAZyme Description: Non-reducing end beta-L-arabinofuranosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
652	MGYG000001311_105|CGC1	73178.4	4.9514

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001311	6514476	Isolate	not provided	not provided

• Gene Location: Start: 35679; End: 37637 Strand: +

Full Sequence      

MKLHPVDLKKIHINDPFWSKHVSLVKEAVIPYQWDAINDRIKDAEPSHSLMNFKIAAGLC
EGEFYGAVFQDTDVAKWLEAVGFALAAQPDGELERTADQVIDIIAKAQCPDGYLNTYFTI
REPEKRWSDLCEGHELYTAGHMMEAAVAYYMGTGKRKFLDVVLRFADLICDTFGFEDGKI
HGYPGHQEVELGLIKLYQVTGESRYLEQAKYFIDARGVGENYFLKELNRPGFRYIFPEFK
DYEPIYSQSHLPVREQKTAEGHAVRAMYMYSAMADLAEACGDDSLMEACRTLWDNMTQKR
MYITGSIGSSGILERFTTDYDLPNDCNYSESCASIGMAMFGQRMGNITREAKYYDVVERA
LYNTVLAGIALDGKSFFYVNPLEVWPDNCLPRTSKEHVKPIRQKWFGVSCCPPNIARTLA
SMGQYIYGVDGESIYVNLFISNETTVDMGGREVSMTMETRFPWKMEVDITLRGVPESGMK
LAVRIPDYAGNFAVRYVGDGGQADGYGPALVREKGYGIISLTGDVCLHVTWDAPARFVHS
NPLVRADSGKVALVRGPIVYCLEEADNGSNLAAVYVDPGTPVTEEEKDLFGGIIGLSFAG
KKISGREWGGDELYGLHPVTWEDVKLKAVPYACWNNRGVGEMVVWMKELFKM

Enzyme Prediction     

EC	3.2.1.185

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH127	12	561	1.5e-207	0.9961832061068703

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3533	COG3533	0.0	2	648	8	587	Uncharacterized conserved protein, DUF1680 family [Function unknown].
pfam07944	Glyco_hydro_127	7.50e-164	11	561	2	503	Beta-L-arabinofuranosidase, GH127. One member of this family, from Bidobacterium longicum, UniProtKB:E8MGH8, has been characterized as an unusual beta-L-arabinofuranosidase enzyme, EC:3.2.1.185. It rleases l-arabinose from the l-arabinofuranose (Araf)-beta1,2-Araf disaccharide and also transglycosylates 1-alkanols with retention of the anomeric configuration. Terminal beta-l-arabinofuranosyl residues have been found in arabinogalactan proteins from a mumber of different plantt species. beta-l-Arabinofuranosyl linkages with 1-4 arabinofuranosides are also found in the sugar chains of extensin and solanaceous lectins, hydroxyproline (Hyp)2-rich glycoproteins that are widely observed in plant cell wall fractions. The critical residue for catalytic activity is Glu-338, in a ET/SCAS sequence context.
COG1331	YyaL	0.004	142	292	415	563	Uncharacterized conserved protein YyaL, SSP411 family, contains thoiredoxin and six-hairpin glycosidase-like domains [General function prediction only].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
BBF45268.1	2.86e-305	1	648	1	640
QAA32625.1	1.15e-290	2	651	8	652
CBL14054.1	1.13e-287	2	649	5	649
QBE96220.1	4.57e-287	2	649	5	649
QMW80468.1	4.57e-287	2	649	5	649

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4QJY_A	3.68e-220	11	648	16	647	Crystalstructure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QJY_B Crystal structure of native Ara127N, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
4QK0_A	1.02e-214	11	648	16	647	Crystalstructure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_B Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_C Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus],4QK0_D Crystal structure of Ara127N-Se, a GH127 beta-L-arabinofuranosidase from Geobacillus Stearothermophilus T6 [Geobacillus stearothermophilus]
3WRE_A	2.10e-153	11	648	3	658	Thecrystal structure of native HypBA1 from Bifidobacterium longum JCM 1217 [Bifidobacterium longum subsp. longum JCM 1217],3WRG_A The complex structure of HypBA1 with L-arabinose [Bifidobacterium longum subsp. longum JCM 1217]
3WKW_A	2.94e-153	11	648	3	658	Crystalstructure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum ligand free form [Bifidobacterium longum subsp. longum JCM 1217],3WKX_A Crystal structure of GH127 beta-L-arabinofuranosidase HypBA1 from Bifidobacterium longum arabinose complex form [Bifidobacterium longum subsp. longum JCM 1217],7BZL_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7DIF_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXV_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217],7EXW_A Chain A, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]
7EXU_A	8.29e-153	11	648	3	658	ChainA, Non-reducing end beta-L-arabinofuranosidase [Bifidobacterium longum subsp. longum JCM 1217]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH8	1.15e-152	11	648	3	658	Non-reducing end beta-L-arabinofuranosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA1 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000062	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001311_00504.