CAZyme Information: MGYG000001313_00495

Basic Information

• Species: Bacteroides eggerthii
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides eggerthii
• CAZyme ID: MGYG000001313_00495
• CAZy Family: PL8
• CAZyme Description: Chondroitin sulfate ABC exolyase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1026	MGYG000001313_17|CGC5	115951.88	7.5705

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001313	4157999	Isolate	not provided	not provided

• Gene Location: Start: 560123; End: 563203 Strand: +

Full Sequence      

MQFSKWTMGAGMLLGTLLPVNAEAQVVKSERLLSFEEPQVPAFISGTGSRLGISGEHYKD
GLHSLSWTFDPGAVLSVKKDLKFEKKDPTGKDTYLSAFIVWVYNEEAQDKKIRFEFLKDG
KKCTSFPFGINFTGWRAAWVCYERDMEGVPEEGMNEMRVIAPDVKGKLYLDHIILASKVD
ARQQAADVQVPFVNKGTTNHWLVIYEHSLWKPDMALTPVSEQQKREVQEIEKRFRDMLYT
PSKFTDKELGGIRKKYDTYKITYKNGKVTGLPIFMVRQAEAYERMIPDWNKDMFTRLGME
MNEYFNLMKRIAIAYNNASDTGVRDELKQKFLAMYDHITDQGVAYGSCWGNIHHYGYSMR
GLFVSYFLMKDVLREAGKLKDAERTLNWYAITNEVYPKPTENGIDIDTFNTKLQGRIASI
LIMENSPEKLQYLRSFSRWLDYGCRPAPGLAGSFKTDGACFHHRNNYPAYAVGGLDGATS
MIYLLSGTEFKLSELAHETVKNVLLTMRFYCNLKQWPLSMSGRHPNGKGELVPIQYATMA
VAGTPDGKQAYDADMAAAYLRLAAYTGMPDKDAPDYLPKASARQELKMKELLEAQGFRPE
PDPQGNLALGYGCVSVQRRDNWMAVVRGHSRYLWAAEHYLPANFYGRYLAHGSMQILTGK
PGEMVTFTTSGWQENGFDWNRIPGVTSIHLPFEQLRAKVLNVDTFSGMEEMLYSDEAFAG
GLSQARLNGNFGMKLHEHDKYNGSHRARKSFHFFGDMIVCLGTDIENTNSEFPTETTVFQ
LAAVTPEARQYWNACKGGGQTYIDPNGVGYYLSKHSMADAKYERNFPQVTVGERSAKPTS
GDWVSLTLQHGKAPEAASYEYAVLPHTDAASLKTFAKNPAYRVLQQDRNAHIVHSLTDNI
TSYVLFETPRSLPADGLLQKADTSCLVMVRENNGKLLLTVSQPDLALYRGSSDEAFDKDG
KRIERSIYSRPWIDNDSGEIPVTVTLKGLWQVAETPYCKLVSADKKQTVLRFTCRDAASF
DVELRK

Enzyme Prediction     

EC	4.2.2.20

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL8	616	864	4.9e-82	0.9959514170040485

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam09093	Lyase_catalyt	0.0	215	549	2	341	Lyase, catalytic. Members of this family are predominantly found in chondroitin ABC lyase I, and adopt a helical structure, with fifteen alpha-helices which are at least two turns long and several short helical turns. The bulk of the domain is formed by ten alpha-helices forming five hairpin-like pairs and arranged into an incomplete toroid, the (alpha/alpha)5 fold. Additionally, two long and two short alpha-helices at the N-terminus of the domain wrap around the toroid. At the C-terminal end of the toroid there is one additional short alpha-helix. This domain is required for degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucoronosyl or 1,3-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups.
cd01083	GAG_Lyase	1.84e-133	252	943	5	693	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam09092	Lyase_N	7.00e-91	30	192	2	167	Lyase, N terminal. Members of this family are predominantly found in chondroitin ABC lyase I, and adopt a jelly-roll fold topology consisting of a two-layered bent beta-sheet sandwich with one short alpha-helix. The convex beta sheet is composed of five antiparallel strands, whilst the concave beta-sheet contains five antiparallel beta-strands with a loop between two consecutive strands folding back onto the concave surface. This domain is required for binding of the protein to long glycosaminoglycan chains.
pfam02278	Lyase_8	1.03e-29	616	868	11	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRQ49163.1	0.0	1	1026	2	1027
QUT44905.1	0.0	1	1026	1	1026
QBJ18580.1	0.0	1	1026	2	1028
QMI79987.1	0.0	1	1026	2	1028
QPH58885.1	0.0	1	1026	2	1028

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2Q1F_A	0.0	12	1026	2	1014	Crystalstructure of chondroitin sulfate lyase abc from bacteroides thetaiotaomicron wal2926 [Bacteroides thetaiotaomicron],2Q1F_B Crystal structure of chondroitin sulfate lyase abc from bacteroides thetaiotaomicron wal2926 [Bacteroides thetaiotaomicron]
7EIP_A	1.34e-79	43	994	53	987	ChainA, Chondroitin sulfate ABC endolyase [Proteus vulgaris],7EIQ_A Chain A, Chondroitin sulfate ABC endolyase [Proteus vulgaris],7EIR_A Chain A, Chondroitin sulfate ABC endolyase [Proteus vulgaris],7EIS_A Chain A, Chondroitin sulfate ABC endolyase [Proteus vulgaris]
1HN0_A	2.49e-79	43	994	53	987	CRYSTALSTRUCTURE OF CHONDROITIN ABC LYASE I FROM PROTEUS VULGARIS AT 1.9 ANGSTROMS RESOLUTION [Proteus vulgaris]
1CB8_A	1.30e-14	643	943	355	639	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	1.34e-14	643	943	377	661	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
C5G6D7	0.0	12	1026	2	1014	Chondroitin sulfate ABC exolyase OS=Bacteroides thetaiotaomicron OX=818 GN=chonabc PE=1 SV=2
Q8A2I1	0.0	12	1026	2	1014	Chondroitin sulfate ABC exolyase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=chonabc PE=1 SV=1
C7S340	9.40e-151	35	992	15	956	Chondroitin sulfate ABC exolyase (Fragment) OS=Proteus vulgaris OX=585 GN=ChABCII PE=1 SV=1
P59807	7.35e-79	43	994	53	987	Chondroitin sulfate ABC endolyase OS=Proteus vulgaris OX=585 PE=1 SV=2
Q59288	7.35e-14	643	943	377	661	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000886	0.997946	0.000292	0.000342	0.000261	0.000239

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001313_00495.