CAZyme Information: MGYG000001332_00638

Basic Information

• Species: Massilioclostridium methylpentosum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium methylpentosum
• CAZyme ID: MGYG000001332_00638
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1194		132240.42	4.3425

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001332	3406391	Isolate	not provided	not provided

• Gene Location: Start: 749425; End: 753009 Strand: -

Full Sequence      

MKRKKIMRISALLVSMVMMMTSVASAFPEEPMSVASYSFKGPSDPAEWGDAQWIWGNENP
GTTDSTNRNVVQYFRRDFKLENLPEVAELQISADTKYWMYVNGKEVVWQGQLVRGPSGPS
HEGNHSGNGDNSKDQTSSRNDGTYYETVDIAEYLQEGENSICILVWHWNNTSTHNNPSRY
GGLLVSSQLPDADNDNNLVYTGDGQWRTRRSPAHNITDTPRSQARLNPTPNITYDARLEI
DWLNPEYSTSDSNGWGNAVSYGPPGGTEDFYGWGPLYERPFGQLKDYGRKALTPVDLSEV
ANPQSRWRTAKAFPAADSGVRRLVTVLPYNCQLVPYIILGEGTPEGQQIVISTETFSPGN
QETVFRTKAGAQTLEGLAWCNGEVLNFDIPEAITDDMIIEVGYRETGYPVSAGEHGKGDF
LGYFNSVIAEDDPSISGQDGDYAIKAFNGGHTWGNEWYATEGPSAENNFYDELWTKAVRT
LYVTMRDTYMDCPDRERGQYIGDAVNEFEEAYYSLGPEADLLTKKAYYEHASWQWEFSWA
GNPDRTYYAMSNVRPGILNQEIYSQSLTAIYSLVDFWKFVGDEEVIQYTWPKYFNYLTNW
DLSTEGDYAGNLRRFRPQGDPPGYNTLSDWCDWGNNQDKYAFYTVIYYMAMKSLYTLGND
PATGVTMTSEQRTFLETRMESIETNFEKFWDEEERAYRSLMGGSHDGQANWSQVSNTCGT
DVDDRVNALAVVAGLVPEERYQDMRDLFMGEGVHTAPHENASIYLEKYVQEALFLMGYDE
DAMLRMARRHMDVVNRTDDTTLPEHWSGGTKNHGWSGGSMIAMSRRVTGVEPTSHAYETW
HIVPQMGNFVSVDTRVPSAIGNIDVKIQKENGVTSMSVVSPGNSAEFWVPLAEGQCAVQT
AGNAKYLGVKEAYGNSYAVFASSEAGTFAFTTADEADKEILKKVIAYAQSESVQEEFDHV
IEAVQLSFTAALDNAVAVDANRDATPEEVESAWKTLLTEIHKLGFVAGDKETLKQLIEIA
ADYEKVLDWYTPNTVEPFTAALAAARATVADGNALQGDVEAAEAALLDAMLNLRYKADKS
VLESVLAKAFGIDTAAYTAESVAAFNAANDKAKAINNDANATQAEVKEAIDTLNAAIDAL
VEINAVPAGTSVNGDTALTTGRSNAKTGDTVPFAAAVTMLALTGFAVIISKKKK

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	462	884	1.1e-49	0.7678571428571429
CBM67	51	266	2.7e-29	0.9772727272727273

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07554	FIVAR	3.53e-07	1096	1141	21	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
pfam08531	Bac_rhamnosid_N	3.75e-07	87	267	6	159	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam07564	DUF1542	6.60e-06	1106	1164	11	68	Domain of Unknown Function (DUF1542). This domain is found in several cell surface proteins. Some are involved in antibiotic resistance and/or cellular adhesion.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QLG44645.1	2.53e-103	50	889	25	717
AXE90460.1	8.78e-100	51	931	42	787
ACU03186.1	3.96e-99	74	866	53	702
AYC36171.1	5.83e-99	51	931	42	796
QBQ39948.1	3.01e-98	46	895	28	731

PDB Hits     

has no PDB hit.

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000322	0.998998	0.000185	0.000181	0.000155	0.000147

TMHMM  Annotations     

start	end
9	28
1170	1189