CAZyme Information: MGYG000001332_00928

Basic Information

• Species: Massilioclostridium methylpentosum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium methylpentosum
• CAZyme ID: MGYG000001332_00928
• CAZy Family: GT51
• CAZyme Description: Monofunctional biosynthetic peptidoglycan transglycosylase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
747	MGYG000001332_11|CGC3	82713.03	5.2127

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001332	3406391	Isolate	not provided	not provided

• Gene Location: Start: 1096056; End: 1098299 Strand: -

Full Sequence      

MRKKSRDLSSGSEFDTKDIFSSTTANKKSRKKPKKQRSTFKRVMIAFGKAIVALFLVGVI
TGSIVVTALTVYVMKATETDSDISLEKDAVMTSGITTIYGQQNGEWVALAKMSGGTKRIW
CDITDVPAHVKNAFVAIEDKRFYEHEGVDFKRTFLAFANMILHFWDSNQGGSTITQQLVK
NLTNDTVQTPTRKVREIFRAMSLERTYSKDQILQAYLNVIQLGGGNGDYLGVATGARLYF
NKEVSELTIAEGASLAAITKSPKRYNPIENPENNLQRRNLVLDNMLDQGMITEEEYTEAI
NTPVEVNRGKVTGTLDDKDYQSYFVDNVINEVIAGMSEKYGWDSAYAETKLKSGGYEIYT
TIDVDLQNKLEEQFQDLSNFRASEQDDDLLAAMVIYDHEGAMKAVVGNRGEKPPGDRVVR
NNATQAERQPGSAIKPLASYAMAIDQNMYDYSNIVSDEETAYKIAGQPDWTPKNYTRRYY
GNVTIKYALQHSLNTIPAKIVNQLGPQTSYDFLTQKLGITTLDPTGDPNMSPMSVGALTN
GVHLNELTNAYQIFTNGGHFVKSHSYTKVLNASGDEVLAPDTTSTQVISEESSSIMNRLL
RQVVMNGGTGAKANLDNLGIELIAKTGTTDDNKDFMFIGATPYYISGLWIGYDNPKNMRD
NTVIPKKYQPYDSSVIWKNIMGDIYQGMEPKEFELTGEVVKKQFCTVTGLLAGDGCPTDT
GYYKASKLPDTCNGGHGGSNPEEGSEE

Enzyme Prediction     

No EC number prediction in MGYG000001332_00928.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT51	110	285	2.1e-61	0.96045197740113

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
TIGR02074	PBP_1a_fam	7.28e-167	117	687	1	531	penicillin-binding protein, 1A family. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of bifunctional transglycosylase/transpeptidase penicillin-binding proteins (PBP). In the Proteobacteria, this family includes PBP 1A but not the paralogous PBP 1B (TIGR02071). This family also includes related proteins, often designated PBP 1A, from other bacterial lineages. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG0744	MrcB	3.33e-162	41	703	5	618	Membrane carboxypeptidase (penicillin-binding protein) [Cell wall/membrane/envelope biogenesis].
COG5009	MrcA	9.53e-127	117	725	66	760	Membrane carboxypeptidase/penicillin-binding protein [Cell wall/membrane/envelope biogenesis].
TIGR02071	PBP_1b	1.19e-91	95	654	136	659	penicillin-binding protein 1B. Bacterial that synthesize a cell wall of peptidoglycan (murein) generally have several transglycosylases and transpeptidases for the task. This family consists of a particular bifunctional transglycosylase/transpeptidase in E. coli and other Proteobacteria, designated penicillin-binding protein 1B. [Cell envelope, Biosynthesis and degradation of murein sacculus and peptidoglycan]
COG4953	PbpC	7.35e-82	102	659	42	542	Membrane carboxypeptidase/penicillin-binding protein PbpC [Cell wall/membrane/envelope biogenesis].

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
CCO04758.1	1.70e-163	31	736	10	740
BCI59848.1	4.05e-147	22	736	12	740
ADU22273.1	2.09e-144	31	732	10	737
QNK41697.1	9.64e-144	34	742	17	714
CBL33874.1	3.74e-139	21	736	18	758

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7U4H_A	3.37e-60	123	715	45	792	ChainA, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX],7U4H_B Chain B, Penicillin-binding protein 1A (Pbp1a) [Chlamydia trachomatis D/UW-3/CX]
3DWK_A	2.91e-59	102	684	13	588	ChainA, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_B Chain B, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_C Chain C, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL],3DWK_D Chain D, Penicillin-binding protein 2 [Staphylococcus aureus subsp. aureus COL]
3ZG8_B	3.26e-57	203	653	3	433	CrystalStructure of Penicillin Binding Protein 4 from Listeria monocytogenes in the Ampicillin bound form [Listeria monocytogenes],3ZG9_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Cefuroxime bound form [Listeria monocytogenes],3ZGA_B Crystal Structure of Penicillin-Binding Protein 4 from Listeria monocytogenes in the Carbenicillin bound form [Listeria monocytogenes]
2OLU_A	1.63e-55	102	684	22	597	StructuralInsight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Apoenzyme [Staphylococcus aureus],2OLV_A Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex [Staphylococcus aureus],2OLV_B Structural Insight Into the Transglycosylation Step Of Bacterial Cell Wall Biosynthesis : Donor Ligand Complex [Staphylococcus aureus]
3UDF_A	1.57e-53	114	687	35	718	ChainA, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDF_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDI_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UDX_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE0_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_A Chain A, Penicillin-binding protein 1a [Acinetobacter baumannii],3UE1_B Chain B, Penicillin-binding protein 1a [Acinetobacter baumannii]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7GHV1	4.62e-89	30	722	3	676	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) OX=441772 GN=pbpA PE=3 SV=1
A5I6G4	6.80e-89	30	722	3	676	Penicillin-binding protein 1A OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=pbpA PE=3 SV=1
A7FY32	6.80e-89	30	722	3	676	Penicillin-binding protein 1A OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=pbpA PE=3 SV=1
Q0TNZ8	4.84e-85	55	719	31	700	Penicillin-binding protein 1A OS=Clostridium perfringens (strain ATCC 13124 / DSM 756 / JCM 1290 / NCIMB 6125 / NCTC 8237 / Type A) OX=195103 GN=pbpA PE=3 SV=1
Q8XJ01	3.13e-84	55	694	31	675	Penicillin-binding protein 1A OS=Clostridium perfringens (strain 13 / Type A) OX=195102 GN=pbpA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999889	0.000131	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

start	end
51	73