CAZyme Information: MGYG000001332_01604

Basic Information

• Species: Massilioclostridium methylpentosum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium methylpentosum
• CAZyme ID: MGYG000001332_01604
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1889		206362.58	4.1665

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001332	3406391	Isolate	not provided	not provided

• Gene Location: Start: 47142; End: 52811 Strand: +

Full Sequence      

MKKSKRILSLLLAACLLATPLASSAVNAAALPDEPGILSSQPTGTLRVGELTVENHQNPL
GVDIAQPNLAWKLSSDGMNQAQTKYRILVASSEQKLDNDEGDLWDTTQQSDKNSCILYGG
TPLQSRQRVYWKVMVWDANGTPSTWSDTAWWEMAFTEESDWKASWIGKDTAVTTKHIITN
SIEPQTARYVRLKVNEVGLRAAAENIQRLQFMEWEIYSSSAPSVNLAAGKSVTSTNQLTP
SGSWSASCLTDGLMDGSHGYSTGGLAGTDLSGDPILITIDLGESTAFDTFKLYGRTDAVS
IDGDICPNYPKQYDVEISQDGETFTPVSSVSIDRESAPRIEGNSTALPLFAKEFEVDKDQ
VASARLYITGLGLYEARINGKNIADTVFDPGETDFKDRVMYVTYDVTDYLNDGGNAIGVQ
LGNGMYNVGSTPGRYQKHDYKLGEDKLLAQLEITNRDGSVQTIVSDESWKQTDGPIVFSS
WYGGEDYDATKEVPGWAEYGTDRSSWSSAGIVDSPTKELSARNMPPLRVQREITPVGINR
LDNGDYVVDMGVNFAGTFDMTLTGLTEEHRGAKIEMWPSELLNADGSVDQGATGSPIWDS
YTVKGTGEESFHLTFMYHGFRYLQVKNCPVELTTDNFRGYFIRTDNDKVGTFETSSDTLN
QINDIINVSVESNMYNTLTDCPHREKLGWLEVSHLMYYSMAYNFDIQSWMKKITLDMTES
QLENGMMPSIAPEYPVFSGGFRSDPTWGGAAILDPWYTYQVYGDDSLLEIAYPMMAKYID
YIKSQSQDNLVNFGLGDWGGYDTSTPLGLVVSATYYNLVDVMSQIADKLNRPEDAQAYRE
LAAEIKSSFNKTYYNEETGQYGSGSQASNACPLFAGLVPDDQIDRVLDNLVANIESRGWH
LSTGEVGLRQMFVVLGKYGRSDVVYKMATNKTMPSYWYFIENGRTSLPEFWDMNASQNHC
MMGHIQEWFYRYLAGIDHAQPGYKQIVIKPYLPDDLEQVKASTMTSYGRITSEWNQDLTS
GRLTMHAEIPVGTTADLYVPALGRTSNTAVINGQTVAGSLEDGYIVYKGLGSGSYDVVRE
PAQVVAINAAIDREILEIGEIAEYAGIAADAQITVTAHTEEGDTLSLAPKAMSYAVDNPD
ILSIDQETGIVKGLRDGIAEITVHYGELSQTLSVRVNTSQVVSIAVSPSKPALNYVGDEV
WLEVTGTIDSGKQIDLTGFSGVELTSDSEEVVIRPSGSMALTERGTVGKPIVVNASFDGL
SGQCTILNGDAPNLARAEETTVRSSSFTKAEWGDYSNESAVDGDRTGNKWGSQGGWSDGT
PGVWPDWYQVDFGSVKTISAIDLTFLQDNFGTAGVPTLETKAARYGMTNYKLQYWDGAEW
QSLAAVTGNDRAWTQASFEPVSTSRVRVLFDAQESGDNQARIVEIEIWESAEPADVSFPV
TFNGTERKDLATAVSAAGSYSVNFDDQKLLEAVEAASGPVRIELSVEQPKINNLLELRSE
TLAALADKEVTFVLRSGVQSVTATANELFNSEAIQANSSVSSPADVFLRLTVGETVTSGV
RYTNFDLTEELPLLPVVETNKTILKAVLDYAAAAIESGQVDSLIPTVKTQFMDAFNHATR
VYESTASTQEQVDQAWPELMKAIHLLDFQAGDKTRLNELIQAAEQLNPDDYDNAQPFVDA
LEAAQAVSLDPDALVGEVDEAVELLEAAMEALILKQWDSLRAAVAYAETIDLTHYVSAGQ
EEFTQALNTALAVLDNSKSTQQEIDSAADSLLNALLALRLKADKTLLSQLVQHVADIDLS
GFSPESVQAFEQALHEAEQLLDDETLTQEEDQGRVDDAVRSLTAAYTGLTSLQGASASKP
AATGEADTLSALICVAAALSLFVLRKKKC

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	538	1038	5e-144	0.996031746031746
CBM67	343	516	1.8e-36	0.9090909090909091

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	4.24e-99	647	973	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	1.12e-69	360	529	2	170	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	9.29e-19	539	642	1	101	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	5.11e-17	975	1044	1	68	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam00754	F5_F8_type_C	1.08e-08	1281	1425	1	127	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SDU81212.1	2.66e-258	21	1428	12	1331
QKG20444.1	4.46e-240	13	1076	8	1051
QKW37237.1	8.25e-234	22	1075	16	1057
QGQ94520.1	1.00e-228	58	1080	17	899
BBA95245.1	2.26e-220	27	1081	47	1121

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	4.63e-133	46	1075	4	1026	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6I60_A	3.55e-132	50	1080	34	942	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
6GSZ_A	5.00e-92	341	1043	124	850	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	1.88e-132	46	1075	4	1026	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KPL4	6.05e-116	58	1078	42	948	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
T2KNB2	4.18e-84	37	1040	23	879	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	1.89e-81	37	1075	26	906	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KM26	2.49e-06	28	137	447	560	Bifunctional sulfatase/alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22250 PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000275	0.998870	0.000231	0.000241	0.000205	0.000169

TMHMM  Annotations     

start	end
7	29