CAZyme Information: MGYG000001332_01733

Basic Information

• Species: Massilioclostridium methylpentosum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium methylpentosum
• CAZyme ID: MGYG000001332_01733
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1658		182177.53	4.3353

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001332	3406391	Isolate	not provided	not provided

• Gene Location: Start: 13634; End: 18610 Strand: -

Full Sequence      

MKKTGFFKKGLAAALAAALLASMLPISAAAREQQQTAPPNNAVATVARGDDAFQKSSHAF
QANWIWSPSDDGQGNRWMSFRKDVHLDSVPEKVTARIAADTKYWLYINGELAVFEGQLKR
GAALLDKDIYLKGTTGQPDLNHMLTQVATYYDEVDLTPYLKEGNNSIVALVWYFGNEGHS
HVGSGKGGFLFESQMGDNLVLSDSSWKVSRHKGYQPSGGIGGYAQEYHINYDARQGFDDF
AQPDFDVSGWEDAAVLGKAGDKPWNELWPRTIPEWKVWDLVTYTPGDEQVTKVDSTHYKV
DLPTNIQFTPYIKVNAPAGRTIKMSCPNSGNTSVTYTTKDGVQEYESPSWINWWFLNYEV
PEDVEVLEFGYRQTGYNTEFEGYFNSDDDFFNTLWTKARDTAYVNIRDTFMDCPDRERAP
WLGDAVNEMMIAYYSMSPTVFDAVRKDISVRVNWQNSDGVIPTTAPTTFRDNEYAELPGQ
SLAGVMSWFQYYLWSGDKQTLENAYPALQSYMEMFDLDDQNYTDPFVRGSGTNTVHHNWI
DWGPNMDQHLSLNIWAYIGVQTLCDFAEALGDDENLAQYQALRDSMKSRFDATFWNGSEY
RSKSYNGPADDRGQALAVYAGLASPDKYPILRDILLNNQYADPYMVKYSIEALYLMGYTA
EAEQRMKESYSRDVPLDDPTFSEGWGGGGTKNHGWSGGGLISLSGYAAGVRPTKPGFEQF
IVAPQMSSIHHIEAGVPTLKGTIQVVADKSENQFDLSVNVPDGSTALIGVSRLENGATMA
VCQNKPVWNNGFVTDAVDGLVFSHTDENFIYFEAQPGDWSLSAQPSITAPKERYTLTIPA
LSGGSALVNGQPVQTPYSQEFVGGAEVTVEAMADEGFVFDLFGGSVGSREAVQNVTMNSD
LLLAPEFVRAWTFEGANLSLSSKTADCKLYVDGRAVMNPYNNLLDRGKSVTIKADAGEEF
RFVGWRSDTGDIVSADPEYTITLDQDISLEAVFVSTLGKNLALGKACTASSNITNNMFDI
SKLTDGVYTITGQNEGWTSLETSPTQWFYVDLGEETCFDTVKLYPRHNGTDNGYGIPLDL
EILISNDAQTWTPVLELKDIERIVSGAHTFKFESQQARYIKFNGSRLRQNPMDGNRTRFQ
ASEVEVYDGSAALTAPVIERQPDNCETRPGTTASFKVSALSVAVAAYQWERSTDSGITWN
EIEGANSATYSFEATLKASGSLYRCTITNEYGSITTDTVSYTAVSDNYALKKPFTYSSNQ
TVPNYFMPEYINDGVTDTLVLVNEGWTSAENHGGSEWVTIDIGEETEVSRVKLYPRYDGT
NNGYGIPIDYNVQVSQNGADWTTVLSATDVERPTSGPLYLDFEPVSARFVKFEGTRLRPN
PMDGNRVRMQIAEFEIYNTHFEPPATNKSILQTVLSYAQSEPVQAEAARAIESVQASFRA
AVEYAAAVNGDVYAEQGEIDAAWMALMTEIHKLGLIAGDKANLSILIQAGETIALENYVQ
AGQQAFTDALTAAKAVLADGDAMQSEVNQAADALLEAMLALRLKADKTLLMRTIAQANAI
DLAGFTPASVNLFERAKAAAEQIAANDALSADDQAVVDRAQRDLADAISGLALSVQATVT
GDTAATASASVPKTGDTLPFAAATALLAAAAFLLRKKK

Enzyme Prediction     

No EC number prediction in MGYG000001332_01733.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	291	768	2.2e-78	0.9920634920634921
CBM67	62	264	8.4e-37	0.9943181818181818
CBM32	1257	1395	3.6e-19	0.9274193548387096

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	1.52e-23	382	628	4	243	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam00754	F5_F8_type_C	3.14e-12	1268	1375	14	113	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	8.18e-12	1008	1124	3	112	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam08531	Bac_rhamnosid_N	2.22e-10	90	256	3	153	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam17390	Bac_rhamnosid_C	1.43e-08	709	766	1	59	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QJT00118.1	1.25e-167	56	1146	36	1117
QTU58279.1	9.02e-167	59	1146	39	1119
AXE90460.1	2.41e-165	59	1146	39	1119
QYX75380.1	6.47e-165	59	1146	39	1119
QEV28934.1	2.40e-164	59	1146	39	1119

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CIH_A	2.56e-21	363	788	283	715	Crystalstructure of a putative alpha-rhamnosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482]
6I60_A	1.33e-20	80	773	179	901	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
2OKX_A	1.21e-17	60	310	6	231	Crystalstructure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A [Bacillus sp. GL1],2OKX_B Crystal structure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A [Bacillus sp. GL1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P9WF03	5.11e-11	155	761	228	869	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	4.57e-06	382	785	490	927	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000327	0.998802	0.000240	0.000236	0.000192	0.000169

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001332_01733.