CAZyme Information: MGYG000001332_02842

Basic Information

• Species: Massilioclostridium methylpentosum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium methylpentosum
• CAZyme ID: MGYG000001332_02842
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1895	MGYG000001332_17|CGC5	205388.51	4.1439

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001332	3406391	Isolate	not provided	not provided

• Gene Location: Start: 729384; End: 735071 Strand: -

Full Sequence      

MKMKQHSKSALALLLAICLMLQMMTGIVAFAAAEPALTVSGLRCDLTENPLGIDNQTPAL
SWKLVSNERGVMQQSYRIQVAESEEELASGDYVWDSGVQTSEDSVNVPYDGPAMTSGTRY
YWQVTVTDNKGNTATSTETAYWEMGLLDESDWEAKWIEINPDNKPNPSSYTIEMDFRIVK
DDAGIIFAGKDGNNFLMWQINTFEKKFNGVTSFRPHQWVNGTPTVLTEKTIEDIIPQDKQ
HDQHHIKIAVDGNKISTWIDNQLINEMTNEYAAYGKLGFRQTTNAGDCDEHATFDNIVVK
TGDEILFQDDFSSGKNSFNIGEIRDGELYVVNALGFQKETGNSAPMYRKSFEVDQPVKKA
TIHASALGIYDLELNGKPVSDDFFNPGWTNYELNQDDNNYVMYQTFDVTEMVQQGENVLG
AITGHGWYSGKLFIGGNNRYGTGSKLLCQLEIEYADGSKKVVATDSSWKITGQGPILEDD
FQGGETYDATREYDGWSAPGFVENSSWTAAKVGSYSGAVVAQMGPTVKAIQEFKPTIVSS
DNGTYILDLGQNIAGFARLNGLKGEAGSTMKLRFGEMLNKDGSLYTANLRSAKATDYYTF
KGDPEGESWQPRFTFHGFRYIEITGYNGTLTEDNITGIALSSLQEQTGSFETSNDLINQL
QSNITWGQKDNFISVPTDCPQRDERLGYTGDGQVFIRTAAMNMNVGPFFKKFMADVTTNQ
RADGAVADWTPNYVTPGDSWSGSFHQTGWGDAVIIMPWTMYTAYGDESFIYDNYEAMKKY
IEYYKNKAGETYVISVGTNGDWLNVNDSTPQNVISTAYFAYVSELLSKMADAIGNTEDAA
YYHQLFLDVREDFNDAFVSADGAVSSNTQTSYLLALKFDLLPTQELREKAAAKLVENIKA
HDWHLTTGFLGVSLLCPVLSDMGYSDVAYRLLQQESYPGWLYSVVNGATTIWERWDSYIA
ETGQFGDVGMNSFNHYSYGSVGEWFYNYVGGIKADEKEPGYKHFTIAPTPGGDLNYANTT
FESMYGTIVSNWKLENGTDFTLDVTVPANSTATLSIPSDMVSAVMESGKNVLEDKVDGIT
FVECKDGKAIFELGSGNYHFTTTVVDKSGLDAAIAKGNSYYEFYYTEDSYKDLQDVVAAA
EALVQDKNAAQAAVDEAIAAIDTAIEDLSRIAQSGTEQDPYLINSVADLQKLARSVNAGA
SFKDEFVKLTADLDLSGIDWTPIGTTGAITAGVGFAGTFDGASHVVSNLSIADTASSATF
GLFGMVSGTVKNLGVKDVQIKVGSGDCRAGGLVGTVNGGLIDNCYVVNANVDAGSRVAGG
LAGQNFNGTIKNSYVKNITVKGGRSAIFVSDNQDDNRNNKGTIDNCYADGSITSGNNGIV
TNSKTIAAAAYTNGELVALLNGSAQTPVWEQGESYPVLIAPAEPVDRTELENAIAEAAEY
EGKEADYTADSWKAFSDALAEANRVLGDSDADQTAVNNAAQELRDAIAGLTPAGPTVNKD
KLNEVLESAKALNQDDYTPNSWAGMDELIAAAQDVYDNSKDQEEVDEMANQLVDKMLSIR
LKANKEELAKAIEAAGKIDQSLYTEESVAAFKEALAEANRIYEDQNISVDDQKLVDDATA
ALNDAIAGLEKIPQPPVDANKVILERVISYAEQAQKSDEYAGAIASVKASFDAALLNAQT
VFADEAATQAEIDSAWISLMNEIHKLGFQAGDKKQLEVVFTQASEIDLSNYVAAGQEEFK
AALANAEIVLADGDALQSDVDEAVDSLLQAMLNLRFKADKSVLQAVLTNADKLDLSAFTA
ETVALFNEAKAAADLINSDENAVQQAVDKAAQDLQDAINGLKAVENTAPATQVEGDQAMT
NGSGSAKTGDTAPVAAAALLAVLACAGFALSKKRR

Enzyme Prediction     

EC	3.2.1.40	3.1.1.73

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	539	1055	1.2e-178	0.9920634920634921
CBM67	341	515	2.4e-35	0.8977272727272727

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	1.58e-132	645	989	1	340	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	9.65e-57	356	531	2	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	8.04e-38	543	641	6	102	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	4.97e-24	991	1069	1	76	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam07554	FIVAR	1.47e-05	1428	1491	1	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
SDT43572.1	0.0	37	1115	1	1061
AVT37932.1	7.80e-273	9	1100	13	1076
AVT31775.1	1.09e-272	9	1100	13	1076
SCG46613.1	6.63e-272	31	1100	33	1073
SDT06954.1	4.82e-258	25	1117	33	1099

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	6.46e-214	36	1101	3	1029	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6I60_A	8.27e-141	42	1100	35	939	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
6GSZ_A	1.10e-140	347	1057	134	847	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	2.40e-213	36	1101	3	1029	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	1.53e-158	48	1100	45	920	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	4.38e-129	49	1109	42	917	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	1.28e-94	49	1098	42	945	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
E8MGH9	6.67e-12	1487	1695	1656	1864	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000325	0.998939	0.000182	0.000220	0.000154	0.000147

TMHMM  Annotations     

start	end
11	33
1869	1891