CAZyme Information: MGYG000001332_02843

Basic Information

• Species: Massilioclostridium methylpentosum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium methylpentosum
• CAZyme ID: MGYG000001332_02843
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1812	MGYG000001332_17|CGC5	197493.83	4.2394

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001332	3406391	Isolate	not provided	not provided

• Gene Location: Start: 735631; End: 741069 Strand: -

Full Sequence      

MRRIQKGIAALLAVFLLASLTLGTVSAAAETLEAAFQILDLTTNNRTNPQGLDVETPSFG
WRMESNVVGQKQTGYHLVVKEKQSGRTVWDSGKTEGEVSQYIAYEGETLEAETDYSWSVT
VYDQTGAARTSETAFFTTGLMADRLIEPEPVPVEDLTEFTVDVDFVIHSGCLGLLLGVQD
SGTFLMWQVHAGDVAINPHRWTGGGLNQNYPRPSIKDALGIDNIIGREMHMTVKANNGVL
TTLIDGTQIGDPVSVGTLKLGRLGFRQFDNEAADVDSISVRDKDGNLVFEDDFNDPSVVN
FSGGRIEDGKLKMNNSRLVFQNADTTGKITAFTLEMDAEVVNDALGIVFGAQDSNNFFMW
QLNAKYGAQGRLRPHRWTGGGVSELNANDLNAVFGDNGPILGTPCHVKIEVDNGVINTYM
GLPGEEMKQVSTVTVEPFELGKIGFRQNRAGGDDEIGIVDNIKVTAKDGAVLFEDDFSDP
SNPGFPSGTITEDGRLRVENALFFQNEKEETVGWSGAQWIGAPEVTFEAGKVPVFEASYT
MQLEQGSTKAAALFCGNDFRLTDKTKNNYRVEGESYLKVELDASGLLNGAPAKLNVYRKG
FCSGDYNNPDATTLLGSFDLNDIGLTQETMYNPIPFSVTAFGYGLSISVGGRSFGQIVAN
PTGRTGDDPMCTYLNQIGFLTEAGQRAVIGDYTIRTRDTAKSMLLSKTSGATYSIFEGTE
GVSVDGGIIRVGSDSAEVKALADPSYGTIQMLRREITAKREISTARLYVTARGVYEMSVN
GVRVGEDWFNPGFTQYDDTITYSTYDITSLMNSGENVIGARLSSGWWCDQMTFDINRYNY
WGNKPSLLAKIVLTYDDGSQDIVVTDPSWTYNNSDSPIIYSGFFNGETYDARREAGIEGW
DTPGYTGANWKQASVITPLPENANPHIIARYDDPVRIAETLSAKFDKEVDPGVYVYDMGT
NMAGVPEIKLPEGKSGQKITIRYSEMYYPELDPSNPFYYGEMGGKLLTENYRGALSTDTY
IMKGTPGGETYRPSFTFHGYRYIEISGFDEPIPAENIKGIVLSSVQEITADYESSNPLTN
QLHLNIRRSLLGNHISIPTDCPQRDERMGWTGDAQVFSRTATYYGSMNTLYEGWENTIRD
AQTESGCIPQTAPNLGWAAGVEVAWPAAIIIPTWEVYKQYGNTRLIEDNLAAMKAFLDSI
DNDRMSPDSYLTRGTALTEHLALIGTDSPLCSNVLYAYMLGLFSKMAEAVGETELAQTYG
DLEVKVKEDWNRVFIDAASGKTQNSGGGIQDTQASYALAIEYGVISDANRDKAVQYLVDA
CARGYNNVPYTITTGFIGTAPLLPALTRSGRIDDAYKMFEQTEFASWLYPVTQGATSIWE
RWNGYTIENGFSGLNYMNSFNHYAAGAVGAWMINDHVGITSDEEHPGFQQFVLQPTPGGD
FTFVNGGYKSIYGQIESNWTASQGELTSYEAVVPANAQATLYLPVDESTLEGFQEVQGVR
YLGMEEHNGRQTAKFSLVAGGYDFALTDGKLTVSVQDGYVTDSEPEPTDTDKTILNQVIA
YAEAAQADASFADVIADVQASFIQTLNEAQKVQKNADASQQEIDAAWIALMGEIHKLGFV
KGDATMLQTLYDYAAGLDMSLYADNQAKADFPAALAAAKSVLDDKDNMLAGEIDAAVDQL
VSVLEQLRFKADKSILESLLEQAGRLDTSLYTAESVQRLNSAVQAGQAVFADGSAEQMDA
DRAAELIRSALDSLEAKSSETTTAEPSAPAQGDAQQTTANQSPATGEALPISAAAVLMLS
AGVFVAMRRRNR

Enzyme Prediction     

EC	3.2.1.40	3.1.1.73

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	948	1483	4.4e-144	0.9940476190476191
CBM67	748	920	8.9e-32	0.8920454545454546

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	1.30e-95	1069	1412	3	336	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	9.45e-60	761	939	2	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	1.76e-27	947	1063	1	102	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	8.36e-16	1426	1510	7	78	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGH33563.1	3.30e-293	491	1524	133	1182
AOZ98905.1	5.86e-225	492	1524	124	1177
AHF25035.1	1.28e-224	514	1533	132	1233
QGH37059.1	7.67e-221	513	1524	141	1180
QOG04730.1	1.69e-218	505	1522	152	1191

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	1.20e-129	744	1486	297	997	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6I60_A	6.01e-103	751	1526	178	941	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]
6GSZ_A	4.59e-100	743	1484	126	847	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	4.88e-129	744	1486	297	997	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	7.00e-120	750	1488	175	883	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	5.40e-112	737	1490	155	885	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	3.92e-61	753	1483	188	906	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1
E8MGH9	1.38e-09	1512	1811	1628	1933	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000369	0.998708	0.000220	0.000249	0.000216	0.000201

TMHMM  Annotations     

start	end
7	29
1788	1807