CAZyme Information: MGYG000001332_02844

Basic Information

• Species: Massilioclostridium methylpentosum
• Lineage: Bacteria; Firmicutes_A; Clostridia; Oscillospirales; Ruminococcaceae; Massilioclostridium; Massilioclostridium methylpentosum
• CAZyme ID: MGYG000001332_02844
• CAZy Family: CBM67
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1841	MGYG000001332_17|CGC5	201993.33	4.2768

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001332	3406391	Isolate	not provided	not provided

• Gene Location: Start: 741210; End: 746735 Strand: -

Full Sequence      

MKGARRMLALLLSFAMTASMASSWNALAAQGDATAILNLRTEDQTNPIGIDDTAPTFSWQ
MKSSELGQKQTAYRVVVATDSELTDVVWDSDRQLDSRSVGIQYQGPPLDPSTVYYWGVTV
WNKDGDVVSSDIAQFETGLLGMEGWNRSQWIQVGTSTEPPEEVLPLNYAIDLDFQVETGG
IAVVFEAQDKSNYLMWQFSVSGDELHFRPHTKKNGGYGVVKDVNVTASADQGALGQQHLR
IEVAGQEVKTYLNGRHIDTTPSSSLNGLGFGTQRGRVGFRSTDSGKEAGYLDNLVITDYA
ADEGGLVTAAYNFDDGKNPFDAGEIKDGRFYTNWTGSSDITGLEPSQQSPDVQDVHYVVE
ADVTCQQDAVSILFNAADPSNFYMWQLNTADRKGSVLLKPHTWKGGAYATYGGHTRNVTA
AVGGVEAFQSTPVHLKLDVTQDEIKTYLNGTLVDTFAIGERSDQGTTGIPVQTGYLGFRS
SANEEGRVDNFALTDYTDNSEGEVLYSYTFDDENDNPFLAGTIENGAFVVKGIDILLPPI
GVPTFRREFIPRQEVVSARLYTTGRGVYEAFLNGERVGQPQPDGRVVYDELKPGFTSPNQ
RASYYSYDVTPMIKQNAANAISATVTSGWWSDAVAWNTGKTSAFRAQLLLTYADGSSQVI
GTDRDWKTTLTGPVIKADIYQGEVYDARCDTSYRESDYDDSGWTYAELHTEFRGVISAQQ
GPAVRVRDDLERSTKSVTVYNGATGATDSQYGKINVTGRYQDGEAFVLQPDEKAVFDLGQ
NFAGWEELELEGAAGTTVTMRHAEMLNDNDGLKSRGNDGPEGSIYTENLRTASAKGVYVM
SGNGVERYHSSYTFYGFRYVEVSATQPVTIHRVKGLVVTSVQRDTGSFETSNQDVNQLFS
NALWGQYSNYLSVPTDCPQRDEREGWTADTQVFSTAACYNADSKGFLEKWMQDMRDCQGS
NGAYPDTAPGGGYMGQLGWADAGIIVPYNVYKMYGDKGIIEDNYASMQRYIDDFLGSTNK
KGGGTAYGDWLAYESNDDQLKGLLGVAYYAWDAQMMAEMAQVMNRPEDVEKYRQVYETEK
EYFQQQYVNSDGSLKIDKQTACLMALKMDLLPDEQSRETVKQMLLDNIARNGDKLQTGFL
GTSVIMQTLSDIGASDVAYQLLLQRGNPSWLYSVDQGATTIWERWNSYTKESGFGDNGMN
SFNHYAYGAVAEWMYGYAAGILYEFDTPGFQHFTLKPMPDQVLGFVNCSFDSPYGMIQSN
WRYDNGSFFYEAEVPANTTATISVPVEEGRELTVNGKAVEELTQQDDGLVYTGTANGRAT
FEAAAGSYRFATTVTQYCYVTLSDAAGGVAGLVRVNGGDPQPMGKTVKLPVGEALTLEAV
PYNDVDYAFSSWTGDVSSTDKTLTVVPQGDMRITANYRWIGRDSLAQGCELSSNAEWAVS
DWALPHLVDGILTSEPGSLGFTSHYTSTPDVDYWIELDLGENKDFNRIQLYPRTDLLTAQ
GETASFPTSFDIQVRRDGETEYTELGRWEDYQAPLRKPAVLSWEQNCNARYVRLHVSKVS
GMPSGEGSYYLQLAELGIYNVSSSEPVDTDKSILRAVLAYADQAKQGEEYANVIDSVRAS
FDAALAEAREIEGSADATQAQIDNAWMNLMREIHKLGFQKGDRAQLELLVTQAGAFDLTC
YVEAGQAAFLDRLSAAQAVLADGDALQNEVDTAAGQLLDAMLALRLKADKTLLNRALDRA
NAVDLTLYSTESLEAFHAAKAEAEQLAADIGLTQEDQPAIDRAADNLHREIDVLTVATAA
VTGDAAVQSGSSSPRTGETTPAAAVLLLLAGVFCLKQRKRR

Enzyme Prediction     

EC	3.2.1.40

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH78	770	1283	1.6e-159	0.9880952380952381
CBM67	539	708	3.1e-26	0.875

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam17389	Bac_rhamnosid6H	1.23e-125	883	1217	1	339	Bacterial alpha-L-rhamnosidase 6 hairpin glycosidase domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam08531	Bac_rhamnosid_N	8.40e-47	554	728	2	172	Alpha-L-rhamnosidase N-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. This domain is probably involved in substrate recognition.
pfam05592	Bac_rhamnosid	1.28e-28	770	878	4	101	Bacterial alpha-L-rhamnosidase concanavalin-like domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam17390	Bac_rhamnosid_C	5.10e-18	1226	1298	5	77	Bacterial alpha-L-rhamnosidase C-terminal domain. This family consists of bacterial rhamnosidase A and B enzymes. L-Rhamnose is abundant in biomass as a common constituent of glycolipids and glycosides, such as plant pigments, pectic polysaccharides, gums or biosurfactants. Some rhamnosides are important bioactive compounds. For example, terpenyl glycosides, the glycosidic precursor of aromatic terpenoids, act as important flavouring substances in grapes. Other rhamnosides act as cytotoxic rhamnosylated terpenoids, as signal substances in plants or play a role in the antigenicity of pathogenic bacteria.
pfam00754	F5_F8_type_C	1.04e-09	1440	1556	10	112	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADD61991.1	8.60e-264	35	1330	20	1145
QQA09206.1	6.59e-263	35	1330	20	1145
QUT40529.1	9.25e-263	35	1330	20	1145
ALJ43908.1	1.82e-262	35	1330	20	1145
QMW84969.1	2.56e-262	35	1330	20	1145

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3W5M_A	2.02e-143	546	1330	306	1028	CrystalStructure of Streptomyces avermitilis alpha-L-rhamnosidase [Streptomyces avermitilis MA-4680 = NBRC 14893],3W5N_A Crystal Structure of Streptomyces avermitilis alpha-L-rhamnosidase complexed with L-rhamnose [Streptomyces avermitilis MA-4680 = NBRC 14893]
6GSZ_A	1.61e-114	545	1301	134	862	Crystalstructure of native alfa-L-rhamnosidase from Aspergillus terreus [Aspergillus terreus]
6I60_A	1.67e-99	543	1285	177	899	Structureof alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12],6I60_B Structure of alpha-L-rhamnosidase from Dictyoglumus thermophilum [Dictyoglomus thermophilum H-6-12]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q82PP4	8.07e-143	546	1330	306	1028	Alpha-L-rhamnosidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=SAVERM_828 PE=1 SV=1
T2KNB2	8.54e-132	546	1297	178	891	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22090 PE=1 SV=2
P9WF03	7.18e-111	546	1285	174	879	Alpha-L-rhamnosidase OS=Alteromonas sp. (strain LOR) OX=1537994 GN=LOR_34 PE=1 SV=1
T2KPL4	3.77e-68	545	1314	187	936	Alpha-L-rhamnosidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22170 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.002496	0.968433	0.001026	0.027163	0.000515	0.000328

TMHMM  Annotations     

start	end
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