CAZyme Information: MGYG000001354_01275

Basic Information

• Species: Bifidobacterium dentium
• Lineage: Bacteria; Actinobacteriota; Actinomycetia; Actinomycetales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium dentium
• CAZyme ID: MGYG000001354_01275
• CAZy Family: GH121
• CAZyme Description: Beta-L-arabinobiosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1918	MGYG000001354_1|CGC31	208278.09	4.3899

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001354	2642081	Isolate	not provided	not provided

• Gene Location: Start: 1565771; End: 1571527 Strand: +

Full Sequence      

MQPRRKKRIVASMGALAAVATLLTGGVVSAYANGATSNPNADMEYPSFKGDADPVPSAGV
SYNPSTSYLKQVFDEDVANGAGNDTSHDFWIDEMLTRKSSEPVGTGSNDAGEYWFEGADR
NEYLFSRGRAAYMYSHKPGSLGFVGNAAYWDETGNDGFTVGVSANGAKIALSEQVDQRKQ
TPSYFTTVFSNADESITITEVKYITYNNVMVANFAVSSATDAEIVLTAASPFAQEGEDGA
AELTGRTNVKNDLTTIYPRFSGNGFTAKDGKLMSTISIKAGEERTTKLQLGLIANELPDS
TGEYEARFNGNLIDPAASYKDSVTTYNQWWYENIPYVETPEHNIDKTVFYRWWLSRFNML
DANMPGNTFQYPTSIEGVLGYNNQIVLTSGMFINDTKWLRNAEYSYGTWVSAGQTAKKGK
SGYYYYHDNPGDPANWNHSYTQYITKAGWDSYMVHGGPSSLAEALGDYGSQDVKGLLNSK
DEPDNNDNQNDNGNSLIDWSWWSMTGNDADAVSFSEPGRSGQRMDRADGSANMWANANAA
AKAYSAAGDTGKAEEMREIADSIKQDVLDNLWDSDSKLLLHKWISDGSFAKYKELNNYYP
YSEGLMPTGNDDYDSALRLFADADEFPIFPFFTANQADKKALNFPGSNNFSIINATPLLQ
IYSAGIRDYNAADNGYITNESYKKLLYWVAFSHYQGGDNRYPDQNEFWNMDNNSSGSTIP
EKNGDASKNGGKITYRSWIHHTQLGTTNWTVIEDVAGVVPREDNKIELNPIEIPDWNHFT
VNNLSYHGKDMSIVWNNDGTYDVPKGYTLYLDGSAVFTSDRLAHLIYDPASGTIEVKDDS
GAKISGAVAASMPNANEVSYGSTSRITQIFAQSGQNVDEASRSQANVAQNADVEATYETK
GYPAANAVDGTNVMESFWGTKGTENAKDSIIVKFKGGAQTIDDVRLYFYQTSSSQTISGY
SEPSVYTLEFQNEAGEWQVLPNQVRTPTYAGANYNRVQFDPVNTQAIRVTFTPQSGMAVG
VKEIQAYDTGIRPDEEPTNATPEVDAYVSSSTSSGAQIVGSVKDDGLPNGEIISKWEQVS
GPENGVARFVDGQATSTTVTFNREGDYVLRLSASDGEKTGEKLVTVHGIPSDGTVNVAPQ
SSATASYTNVWQPKDNAKKVIDGQVVYTNTPNETWNNWGDSTGEDATLQLEWDGSIPLKK
AKLYFWTDHGGVPMVKSWRLQYADADGNWKDVKLADGQSYTTVENQGNEVKFAETVETSK
LRIVFPKGSYVGVSEFEAYALDPVSVSDVNRMVKTGTKASDLALPTMVSAAYTDGTRRNL
AVTWGKVTDEQLASDSRFTVSGVVAGALAGAKAIIGVRSDAQAQISGAAQPLEQTVYQNS
KAIDLPTVVPVRFPNGMLDDRKVVWRDESVKSIDLTKIGDYEVRGSAEASSSEAKITVHV
VADPSDSVTPDPKPGPDPEPDPGPLEGWIEGKASDTTVSAEASWSPAAGKLNDGKLVDDN
FPTGDDQDVNAYVWGTWGKASSGMYAQYTWNQTALVDSSRVQFWANMTETNKTLGGLEIP
TEWKIQYLAEDGTYKDVENAKYTVVRNDPTHHATDGAKGWSEAIFTPVKTASLRLVLEPY
DGGTGAGTFGAAVVEWGVHAVKEETPDPPTPAVDKTKLESAIKAAETLEESRFTSASWSE
FAAILQASKTVLDNVDATQDDVDNAVSKLEDRQKALVARGDKTDLRQSFDNADAIDSSRY
TDKSVEALKEAMHIASDVLRNEDATQSEIDEATKTLMATINGLETKGDPEPVVVDESALK
TLVARADALKGSDYTEASWKTFSDVLAKAKSVLMDDAVSQSDVDAAASNLEKAASALVRK
DGKSVKVDISTGGQVSGKSQAIGNTGSNVAVLAVMLLVAVVAGVIVLLIVNRRHGTER

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH121	7	1443	0	0.9964080459770115

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam07532	Big_4	1.32e-07	1286	1346	1	58	Bacterial Ig-like domain (group 4). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.
pfam07532	Big_4	1.15e-05	1370	1428	2	57	Bacterial Ig-like domain (group 4). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.
pfam07554	FIVAR	7.99e-05	1797	1858	1	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.
pfam00754	F5_F8_type_C	5.89e-04	896	1012	6	113	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam07554	FIVAR	6.28e-04	1723	1784	1	69	FIVAR domain. This domain is found in a wide variety of contexts, but mostly occurring in cell wall associated proteins. A lack of conserved catalytic residues suggests that it is a binding domain. From context, possible substrates are hyaluronate or fibronectin (personal obs: C Yeats). This is further evidenced by. Possibly the exact substrate is N-acetyl glucosamine. Finding it in the same protein as pfam05089 further supports this proposal. It is found in the C-terminal part of Bacillus sp. Gellan lyase, which is removed during maturation. Some of the proteins it is found in are involved in methicillin resistance. The name FIVAR derives from Found In Various Architectures.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
VEG24276.1	0.0	1	1918	1	1918
ADB10293.1	0.0	13	1918	1	1906
BAQ27605.1	0.0	13	1918	1	1906
AZN74101.1	0.0	5	1791	6	1801
BAR02807.1	0.0	5	1791	6	1801

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6M5A_A	0.0	40	879	9	865	Crystalstructure of GH121 beta-L-arabinobiosidase HypBA2 from Bifidobacterium longum [Bifidobacterium longum]
5MQS_A	2.74e-15	304	830	629	1106	SialidaseBT_1020 [Bacteroides thetaiotaomicron]
5MQR_A	4.68e-13	304	830	629	1106	SialidaseBT_1020 [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
E8MGH9	0.0	1	1918	1	1940	Beta-L-arabinobiosidase OS=Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b) OX=565042 GN=hypBA2 PE=1 SV=1
Q9L7Q2	2.52e-09	1653	1851	416	618	Zinc metalloprotease ZmpB OS=Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) OX=170187 GN=zmpB PE=3 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000818	0.997844	0.000366	0.000479	0.000251	0.000203

TMHMM  Annotations     

start	end
9	31
1889	1911