CAZyme Information: MGYG000001364_00753

Basic Information

• Species: Phocaeicola plebeius
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Phocaeicola; Phocaeicola plebeius
• CAZyme ID: MGYG000001364_00753
• CAZy Family: GH97
• CAZyme Description: Retaining alpha-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
645	MGYG000001364_3|CGC1	73655.09	6.6039

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001364	4421324	Isolate	not provided	Asia

• Gene Location: Start: 20665; End: 22602 Strand: -

Full Sequence      

MKNLSLTLGLWVLGAAMCAAEPIELDLSSPEGTHKVKFYEKKSASGANVLCYEVNYNGKQ
VVKESQAGLQLDNRVWEMALGMRKLPQPACWMDNLEVDSVAYGSHDATWTPLYGERSSVR
DNYKWGTLYLSKKDGSNYRLNVEVRAYDEGVAFRYYFPEHPKAIFHKVVGDLTEYTFPEG
TKAWAEQWAQAHFEYLDVNALKKPVERALTLELPNGVWAALTDADVDDWCLTKFQSSAEK
QYTLTSVMYSPVDIVTYYATPWKVIMAADKPGELLEHNYIIENLNPPCEIKNTNWIKPGK
IMRETSLTTDQALKTIDFCAEHRIPYMLFDWQWYMPCTSHDGDATKVVSKLDMPKVVAYG
KKKGVGIWLYVNQHALQKQMRELFPLLRKWGIVGVKSGFVQYASHRWATWLHDMVRLAAE
NNLMMNIHDEFRPSGFSRTYPNLLTQEGILGNEEFPDATHSTILPFTRMINGAADYTICY
YDKRLKNTHAHQLAASLVFYSPLMTIFWYDRPQFYNGEPEIEWFENLQTVFDESHVLNGM
PGKNITMARRKGKEWFVGMLTNNDGSDETLDCSFLGKNQMYLASIYTDGGEEVKTKTQVK
CSYVLVGAEDVIKFTLKPRGGAAVRLVPVTEKEAKAYKKYGGESF

Enzyme Prediction     

No EC number prediction in MGYG000001364_00753.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	19	626	2.1e-164	0.9825673534072901

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam14508	GH97_N	4.75e-64	29	287	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam10566	Glyco_hydro_97	8.24e-57	293	520	2	269	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14509	GH97_C	1.43e-31	531	627	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ALJ62032.1	0.0	1	643	1	645
QDO69864.1	0.0	1	643	1	645
QUT92217.1	0.0	1	643	1	645
QUT34121.1	0.0	1	643	1	645
QUU00177.1	0.0	1	643	1	645

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5E1Q_A	3.12e-58	50	626	35	654	Mutant(D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482],5E1Q_B Mutant (D415G) GH97 alpha-galactosidase in complex with Gal-Lac [Bacteroides thetaiotaomicron VPI-5482]
3A24_A	2.36e-57	50	626	21	640	Crystalstructure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron],3A24_B Crystal structure of BT1871 retaining glycosidase [Bacteroides thetaiotaomicron]
3WFA_A	2.53e-45	27	639	5	717	Catalyticrole of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron],3WFA_B Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron]
2JKA_A	2.56e-45	27	639	14	726	Nativestructure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKA_B Native structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKE_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKE_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKP_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482],2JKP_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482]
2D73_A	2.80e-45	27	639	25	737	CrystalStructure Analysis of SusB [Bacteroides thetaiotaomicron VPI-5482],2D73_B Crystal Structure Analysis of SusB [Bacteroides thetaiotaomicron VPI-5482],2ZQ0_A Crystal structure of SusB complexed with acarbose [Bacteroides thetaiotaomicron],2ZQ0_B Crystal structure of SusB complexed with acarbose [Bacteroides thetaiotaomicron]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8A6L0	3.63e-57	24	626	22	661	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
G8JZS4	1.53e-44	27	639	25	737	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
D7CFN7	1.18e-39	50	558	59	560	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000314	0.999038	0.000185	0.000156	0.000142	0.000140

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001364_00753.