CAZyme Information: MGYG000001368_02417

Basic Information

• Species: Muricomes fissicatena_A
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Muricomes; Muricomes fissicatena_A
• CAZyme ID: MGYG000001368_02417
• CAZy Family: GH86
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1349	MGYG000001368_5|CGC2	150811.41	4.2768

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001368	5374587	Isolate	not provided	not provided

• Gene Location: Start: 45843; End: 49892 Strand: +

Full Sequence      

MKHTRIRRIIAVLLCVSVMLPTALSNKPVQAEDRQSDLPEDIIENKISFDTVDTVIEKYK
TEFSNREIDPIAYKSSINGENRVRRINFYFPEEPLQVNGECEIPIPEEIARILRLFGDVR
YTGSIADSGTCKMYIVDPDGKEHLLPNYGQAWKGEFSIGGEPQWTSYGKTSANESSLMNP
TEHKVNGEVNYTGYKLKFLGTGSLNFINLWEENGLPMEYDTSAYGVIGSDKDVIDATVSF
DATKNLSIDGVNKMEDDVFKRVHVNSGPVQVFSNMNTPILDDEVFSKTYRDWKFTPGRGT
FQIKDAGGLLTEGADGYSNTDALFESYALNPVMVDKLNTLFPATQDDYILTYDGWPEWQW
GELGDINIKNINMATPDYKYFKAAADTAAKLTKAIDTRFDGYGPRYIEVKNESTFSGEWA
FFNTCDEDTAWNYLAEFHNEVADAIKKEVPEVLVGGPSSAFMYIEKDDFKDAFRQLKFMD
DTKGHLDWYSHHFYENSASLYVNGREENSDGFLCGRFQAVMDLLRAHMENTDNVRPILIT
EEGSYNSLNTDIDYFQNLTAFNGYFIQSLEYADTIDSLIPYLYPVCSWRPNGNNQLYKYN
EGQTGILEEMTPLEAYVDMWTDFNGAFIPASISTDDPVLKSRIYTKAVRQGDTIYLAVQN
LNNQQVNLDIEGILGNGIQIENISEKHYFLENAKLTYEEIMVDDLNDIHMRVQEMAMFEI
KVSDAAEFVSEVYKEVDYAREELVETGNHMEFTVESSKSDLVDSTIRVGFGKSGSGFAGT
MTISLNGKVVGNRDLEYTNKTGDIFTTADFDIDPAEVKEGTNKIVVSMPEGGRVSCVSII
NNYDKAAAACADAAPLKVKIEEALNMIGDKLYLSEDGSGLPAGAKWITQINFESFTNVIT
DACALLKDVMRTDKEIQTQLKILDTAMTMFSKNSKDAVVIESEAAVFPDTTFEEGEDVSY
IVTNGKPAESEIITENNNSVLKVTVPKMGSWNDLTQVVWTAADGEYWDLMSNHLTFDIGN
PNEYTLYYAVNIYDTEGGLTQNWLQVAAGETKHVELIPAAPANFKNAAKVAIWINEPHME
MKDKENVSFILDNVCVIKEDEEPTVLVKAERPDVDTTIVEGLKLPEEPEIIEGANAVWDN
GTKGNLIIKSDALFADFIKVTVNGTDLNSECYEVSEGSTVVTLKESYLDGLEAGVYNIAI
VSKSGIAETTFTIKRSEKPDGGETDDKELVSIEITKEPNKKIYQVGEKLDITGMEVMARY
EDGTTELISGYQVKGFDSSKSGDIYLTVVYEGKSCRLKVTIRENAGSETVKKPDVIKTGD
ESRIFVAVVALVLSAIVLLMLLLRKRLKK

Enzyme Prediction     

No EC number prediction in MGYG000001368_02417.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH86	236	840	8.7e-79	0.9830795262267343

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd21510	agarase_cat	5.36e-70	345	620	45	320	alpha-beta barrel catalytic domain of agarase, such as GH86-like endo-acting agarases identified in non-marine organisms. Typically, agarases (E.C. 3.2.1.81) are found in ocean-dwelling bacteria since agarose is a principle component of red algae cell wall polysaccharides. Agarose is a linear polymer of alternating D-galactose and 3,6-anhydro-L-galactopyranose. Endo-acting agarases, such as glycoside hydrolase 16 (GH16) and GH86 hydrolyze internal beta-1,4 linkages. GH86-like endo-acting agarase of this protein family has been identified in the human intestinal bacterium Bacteroides uniformis. This acquired metabolic pathway, as demonstrated by the prevalence of agar-specific genetic cluster called polysaccharide utilization loci (PULs), varies considerably between human populations, being much more prevalent in a Japanese sample than in North America, European, or Chinese samples. Agarase activity was also identified in the non-marine bacterium Cellvibrio sp.
pfam07523	Big_3	1.15e-08	1237	1301	1	67	Bacterial Ig-like domain (group 3). This family consists of bacterial domains with an Ig-like fold. Members of this family are found in a variety of bacterial surface proteins.
pfam18206	Porphyrn_cat_1	1.34e-04	643	739	2	100	Porphyranase catalytic subdomain 1. This domain is found in porphyranase protein present in Bacteroides plebeius. Porphyranase breaks down porphyran during digestion of red seaweed glycans. It is worth noting that red seaweed glycans contain sulfate esters that are absent in terrestrial plants. This domain makes up part of the catalytic domain of the porphyranase protein.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QCT03653.1	6.48e-258	1	1090	1	1089
AML39432.1	2.59e-257	2	1103	24	1124
BAT46645.1	2.59e-257	2	1103	24	1124
AYA22371.1	1.47e-250	10	1090	2	1083
QGG56411.1	3.43e-249	3	1090	4	1092

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5TA1_A	7.18e-14	434	839	222	637	Crystalstructure of BuGH86wt [Bacteroides uniformis],5TA5_A Crystal structure of BuGH86wt in complex with neoagarooctaose [Bacteroides uniformis],5TA5_B Crystal structure of BuGH86wt in complex with neoagarooctaose [Bacteroides uniformis]
5TA0_A	1.64e-13	434	839	222	637	Crystalstructure of BuGH86E322Q in complex with neoagarooctaose [Bacteroides uniformis],5TA0_B Crystal structure of BuGH86E322Q in complex with neoagarooctaose [Bacteroides uniformis]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000384	0.990851	0.008098	0.000252	0.000217	0.000178

TMHMM  Annotations     

start	end
1324	1343