CAZyme Information: MGYG000001368_03928

Basic Information

• Species: Muricomes fissicatena_A
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Muricomes; Muricomes fissicatena_A
• CAZyme ID: MGYG000001368_03928
• CAZy Family: GH110
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2076	MGYG000001368_11|CGC2	227107.25	4.6412

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001368	5374587	Isolate	not provided	not provided

• Gene Location: Start: 66238; End: 72468 Strand: +

Full Sequence      

MGSKRTKAVKGVRTAKRLIALGIVICMVLSQGLLVLAKAPKAAGKVINVLDYGADPTGIK
DSTVAIQKALKAAKKAEDDGRNQVTLNFPKGEYHIYKDYAEVREYHTSNTNSIEHPEKTI
GILIEEHKNLTVEGNGSLFMMHGNMMALAVVKSDNITLRNFSWDFAVPTVSEMTIMDMGM
DGGQAYTDFYIPKCFPFEISGSTIKWYSEKSPYTGEYYWTETGIHNAYSIVGYQPDEEMT
RAYFTSETPFQNVSSISKISDTVVRITYTGNNRPAMQKAGMVLELASSAVRETAGAFTWE
STNVLAEKVNVHFMHGFGWLIQMSKDVTYRECNFMPRKNSGHITVSYADIIHASGAAGQI
LIEDCNFSNSHDDPINLHGTFTRVENRVDDHNLVLKYIHQQQGGFPQFHVGDKVAFFTRD
TLESTDNETLYTVEAVTDPGQDGNDLRTMQVRFKETLPVDLSDRIGSEPKYVAENVTYAP
AVTIRNNTFKNVPTRGILCTTRNKVLIENNTFLNMSMATIYLSNDSDQWYESGPIRDMEI
RNNTFHIKNIGRTSWEYAPAVYFHPVTKGGGLPSADNPIHKNITIEDNTFHMDVDTVVKA
ESVENLTIRNNKVWRKNPDVSIQIAEDKNQLAVGEVGAVTTTATGDTNTRPQDNVFEFTK
CKNVILEGNTYDDGLKKYAVLSGMPESYLTNKDSDIKVMDNRDGVVTDPVRNIRYASTDP
SVVTVDDSGKFYAKKQGTAEVYAYYVWNDTIIKSNAISVTVSADTAVKPSLEITTEKPVI
LEQPDEQKTITATTSSSSAISWSVSDFLTGGATEVATINESGVVTAKQNGIVWVKASVEG
METEIPVVVSLPTEKVNPAFSIVRESKGNYELNSDSLTINMQSGDLYQSDNNVQNLFLYT
LPDSIDKNAMRTIVKVDNLPVRESDQWDTGAFILYEDDNNYITIGKKSHYDGITSVREVG
GAAVETGGNSSENSVSTALLGFTKNGDSVSLDFKVGNGEWQKLRDLDASMLGDNYKIGFA
TWGNDERGTKVTFSDFRAAGSDVSYQDLENQATISWGNVNQAPEVNNLHFSKESYYVGES
AEISYDFSDADNDSEGASLYLWNYTQDGVEKQEVTDVPRFTAKASGTLKCEVYPKDAGGA
PGYPAQQSISVEKGSVDLTLDSLEINGEELLKDDMSKRSFDVYLPADLTKVELSYHSIMP
DEGTIKISVGDKAVTADPQNVGSLVLESLKDKDVISVERGKDVIYKINVHSIESNNVAIK
GITMPEIGLDISDFTPGNWLVNTEASGSMINITADASIGKVELQSDHYRKPVPFTKSGEQ
YSAEVNFRNGLNTYYVVVTAKDNITTKKYIINVIYNANSDVTVQNITLNGSSIDGFDPDV
YEYVTELPEDASKLAVAAESDGSLRLAINQDTVIGSELESTSLKNGVNDVYVMALAGDGI
TRKTYHIKVVKPYKENTNVLGIALNEKDIADKFDDRGKLSTTIPGDKVVMKIQAEDKGAD
IKVSSGKEVFKGKGSLIQSLNIYKETPAIDIEITARDGKTKKSYYIDLEKAVYLSDLDWK
SGATVGYDQIQKDKASSGSVIRIPDKDGNPVTFEKGIGTHAESIISYDVAGLGVNKLQGY
VGIDYAKYGSNESSVQFQIFAGDTKLFDSEVMLSTTPMKKFDVDIGNTDMITLKALKGEN
NWSDHADWADIKLTADFKATPVKYYAQTITPAEHGVITSSVEGNKLLENSSVTYTFTPDK
GYFVKEVVVNGQKVMLTGDQYTVDTVTENITVSAEFAKGEEPVKYYSQTITSAENGVITS
STEGDALREHSSVTYTFTPDAGCHIIEASVNGQVVLLEGSQYTVADVMENITVSAVFAKK
ESQAVIEDVNGTGIKAEIPKELLGDANPTLNITPVGDSEESYKELAASLVGKEDILGAYQ
VALEGLPEGVDVSAVTLHFPVDSKYNGKRAVVKLLTSDHTIVSWETEIKDGQVTIENPME
LATFMVAVDPGKKPEPEKPDPQNPGPQKPDPQKPAPQKPDTPKPDPQKPDTSKPGQTTGK
PSAAKTGDASPVAATLIGLMAGGAVIAITVKKKRQI

Enzyme Prediction     

No EC number prediction in MGYG000001368_03928.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH110	46	621	4.9e-152	0.9598540145985401
CBM51	1552	1693	3.6e-36	0.9925373134328358

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam08305	NPCBM	9.46e-38	1550	1694	1	136	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
smart00776	NPCBM	5.27e-35	1549	1694	2	145	This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins.
NF033839	PspC_subgroup_2	1.63e-10	1990	2074	429	555	pneumococcal surface protein PspC, LPXTG-anchored form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site. The other form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A.
NF033839	PspC_subgroup_2	3.80e-08	1990	2041	407	462	pneumococcal surface protein PspC, LPXTG-anchored form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site. The other form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A.
NF033839	PspC_subgroup_2	1.27e-07	1990	2042	396	452	pneumococcal surface protein PspC, LPXTG-anchored form. The pneumococcal surface protein PspC, as described in Streptococcus pneumoniae, is a repetitive and highly variable protein, recognized by a conserved N-terminal domain and also by genomic location. This form, subgroup 2, is anchored covalently after cleavage by sortase at a C-terminal LPXTG site. The other form, subgroup 1, has variable numbers of a choline-binding repeat in the C-terminal region, and is also known as choline-binding protein A.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYE35188.1	0.0	45	1384	44	1394
QAS60591.1	0.0	45	1384	44	1394
AGN25168.1	9.63e-252	45	1368	34	1301
VEH83785.1	1.88e-251	45	1480	34	1436
QDS38485.1	5.11e-251	45	1368	34	1301

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7JW4_A	3.28e-43	30	611	11	564	Crystalstructure of PdGH110B in complex with D-galactose [Pseudoalteromonas distincta],7JW4_B Crystal structure of PdGH110B in complex with D-galactose [Pseudoalteromonas distincta]
7JWF_A	1.42e-42	30	611	11	564	Crystalstructure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_B Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_C Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_D Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta]
2VMG_A	2.25e-23	1538	1694	1	157	Thestructure of CBM51 from Clostridium perfringens GH95 in complex with methyl-galactose [Clostridium perfringens]
2VMH_A	4.79e-23	1546	1694	3	151	Thestructure of CBM51 from Clostridium perfringens GH95 [Clostridium perfringens]
7JRM_A	6.48e-23	1533	1770	63	283	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B1V8K7	8.68e-117	46	764	34	726	Alpha-1,3-galactosidase A OS=Streptacidiphilus griseoplanus OX=66896 GN=glaA PE=1 SV=1
Q826C5	2.71e-106	37	615	31	585	Alpha-1,3-galactosidase A OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=glaA PE=3 SV=1
Q64MU6	2.14e-79	47	615	26	552	Alpha-1,3-galactosidase A OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=glaA PE=3 SV=1
Q5L7M8	1.32e-78	47	615	26	552	Alpha-1,3-galactosidase A OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow) OX=272559 GN=glaA PE=3 SV=1
Q8A2Z5	7.70e-76	66	614	28	533	Alpha-1,3-galactosidase A OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=glaA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.789296	0.206807	0.002051	0.000229	0.000202	0.001416

TMHMM  Annotations     

start	end
20	37
2051	2070