CAZyme Information: MGYG000001371_01120

Basic Information

• Species: Paenibacillus lautus_A
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus; Paenibacillus lautus_A
• CAZyme ID: MGYG000001371_01120
• CAZy Family: GT2
• CAZyme Description: Dimodular nonribosomal peptide synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2409	MGYG000001371_27|CGC1	267802.73	4.9033

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001371	6950059	Isolate	not provided	not provided

• Gene Location: Start: 24945; End: 32174 Strand: -

Full Sequence      

MLEQKRERWSLSGAQLGIWYAQQLDPDNPIFNTAECIEIRGPVDPVLFEKALRQVVGEAD
ALFARFGEDADGPWQSIEPSPDWPLCVMDVSGMEDPHLAAQTWMEHDLAQAIDLSRGPLF
TEALLKLAADRYYWYQRIHHIAIDGFGVSLLLQKVSEVYTDLTGGGASGIGAFGRFRTIL
EEDASYRMSQQLGSDRQFWLQRFADEPEVVSLGGQAQRSARSFLRRSAQLPASSMQRLQA
AAQAAGTSWPDVIAAAAALYVHRITGAEEIVLGLPVMCRLGSSSIRIPGMVMNLLPLRVR
VRPDMTLIDLLRQVARENREIKRHQRYRHQDIRRDLKLLADNRRLFGPLVNVMPFAEELN
VGGFRGSVRNLASGPVDDLTMNVYGGLGGHGLRIDMDANPMIYGAAELESHLQRYLYLLD
SLAEAEPNDPVGGLAFILPDEREKALVRWNDTTRALPQLSVPQLFEEQAARTPQAEAVIC
EGLSLCYEDLNRRANRLARLLIAREIGPERIVALSLPRSVDMLVAILAVHKAGAAYLPLD
PDYPDERIAYMLADANPACLITTRQLSEHFASVMEDKGVILLDAPATLQRLEQQVFHNPV
DADRVRPLLPLHPSYVIYTSGSTGRPKGVMLTFEGLANLLSDMRERLGIGERDRWLSVTT
MSFDISVMEMLLPLACGSTLDIVMRDTILDAAALIRRIRETRTTIMQATPTLWQSIVACR
PGKFERLKVITGGEALPVSLKLALQDLNCEVNNQYGPTETTIYSTAAKLDQAPEKPSIGG
PLWNTRLYVLDSSLSPVPPGVTGELYIAGLGLGRGYLGRPDLTADRFVADPYGPPGSRMY
RTGDLARWQENGWIDYLGRADHQIKLRGFRIETGEIESVLVAHPAVEQAFVMVREDRVGD
RRLVAYIVPSSDWPHPIDSTDLRSYAAEKLAEYMVPSVIAALEALPLTPNKKVDRKALPV
PDVRLAAGRMPRTPQEEMLCTIFGEVLGLPRVTIDDDFFELGGHSLLAGRVMVRIREAFG
AELSIGSLFEASTAASLVKRLDHAQAAREAVRPAARYERLPLSFAQQRLWFLYRLEGPSP
TYNIPLVARLSGDLDLASLRMALWDVVARHESLRTVYPENDGASYQRILDAERARPELMV
TEIREAELADRLADAVRYRFELAAETSLRAELFKLGDQEFVLLLLLHHIAGDGWSLNPLA
RDLADAYAARCLGSEPTWTPLPVQYADYAMWQQKLFGRSGDENSLMDRQLAYWTEALSGL
PEQLELPTDFPRPAVASYQGGSVPFSISRELHLQLNGLAQSNRATLFMVFQSAMAALFTK
LGAGTDIPIGSPVSGRNDDNLDHLIGFFINTLVFRVDTSNDPTFDELLRRVREKSLAAFE
HQDVPFERLVETLNPPRSRARHPLFQVMLVLQNTPETSLELPGISSKLQLQSVGTAKFDL
TFELTERRDQNGEPAGVDGLIEFSSDLFERGTAEAMAERYVRVLESAVVEPSKSISKLDI
LTVDEREDIRQQWSAPLPVTEQATLSERFEAQVSRSPQSVAIVHDGQSLSYEELNERANR
LAHFLIASGVGTEQIVALALPRSVDMVVGILAVLKAGAAYLPLDPEYPEDRLAYMMEDAC
PVCVMTSVHVMDQLPSFGRWLRVVIDGPEEAMQWQRYSKENPSDRERLGGLMPQNAAYII
YTSGSTGKPKGVLVPHQNVVRLFDSTRHWFQFNETDVWTLFHSYAFDFSVWEIWGPLLYG
GRLVVVPHTVSRSPEEMLGLLVREGVTVLNQTPSAFYPLIQADREQPETGRQLSLRYVVF
GGEALELGRLTDWYRRHADDAPRLINMYGITETTVHVSYMELNRNLALPGAGSLIGEAIP
DLRVYVLDDRLQPVPYGVIGEMYVAGAGLARGYWGRPDLTADRFVADLYGPPGTRMYRTG
DLARRFADGTLDYLGRSDHQVKIRGFRIELGEIESVLVRHEALSQAAVIVREDQPGDQRL
AAYVVGNPGESPSGTELRRYAASLLPDYMVPWAFVLMDKLPLTPNGKLDRKALPAPDMSP
GADGRNPRTPQEEVLCELFAEILGAKRVGIDDSFFELGGHSLLAVRLISRVREALGKELS
IAALFEAPTVAGLVDKLEMGGGNSALQVVLPLRSHGDQTPLFCVHPAGGLSWCYAGLMKH
LGMNYPIYGLQARGIAHPEELPGSLEVMTADYIGHIRSLQPAGPYRLLGWSLGGNVAQSI
AVQLQEAGEDIELLAILDAYPSHYLPIKEKPDEDEALTALLALGGFDRESIEAGLGDAPL
TIAAALELLRNESSALSSLEEETIMNLKTTYENSVQLLKAYVPKRFEGDMLFFHSTIIPD
WFDPIDPEMWEPYVGGTIERHDIECRHKDLCQPGPLDEIGKTILNKLEEMASKTETTRDR
RELIYDQSI

Enzyme Prediction     

No EC number prediction in MGYG000001371_01120.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF038078	NRPS_MxcG	0.0	7	1047	3	1049	myxochelin non-ribosomal peptide synthetase MxcG.
PRK05691	PRK05691	0.0	12	2113	679	2771	peptide synthase; Validated
PRK12467	PRK12467	0.0	1040	2241	2627	3795	peptide synthase; Provisional
cd05930	A_NRPS	0.0	1537	2033	1	444	The adenylation domain of nonribosomal peptide synthetases (NRPS). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
cd19540	LCL_NRPS-like	0.0	1059	1491	1	433	LCL-type Condensation domain of nonribosomal peptide synthetases (NRPSs) and similar domains. LCL-type Condensation (C) domains catalyze peptide bond formation between two L-amino acids, ((L)C(L)). C-domains of NRPSs catalyze peptide bond formation within (usually) large multi-modular enzymatic complexes. NRPS can use a large variety of acyl monomers (approximately 500 different possible monomer substrates as opposed to the 20 standard amino acids in ribosomal protein synthesis) to construct bioactive secondary metabolites of 2 to 18 units long (with various activities such as antibiotic, antifungal, antitumor and immunosuppression). In addition to the LCL-type, there are various subtypes of C-domains such as the DCL-type which links an L-amino acid to the D-amino acid at the end of a growing peptide, starter C-domains which acylate the first amino acid with a beta-hydroxy carboxylic acid, and heterocyclization (Cyc) domains which catalyze both peptide bond formation and cyclization of Cys, Ser, or Thr residues. Typically, an NRPS module consists of an adenylation domain, a peptidyl carrier protein (PCP) domain (also known as thiolation (T) domain) and a C-domain. NRPS modules may also include specialized domains such as the terminal-module thioesterase (Te) domain that releases the product via hydrolysis or macrocyclization and any of various C-domain family members such as the epimerization (E) domain, the ester-bond forming C-domain, dual E/C (epimerization and condensation) domains, and the X-domain. C-domains typically have a conserved HHxxxD motif at the active site; mutations in this motif can abolish or diminish condensation activity. An HHxx[SAG]DGxSx(6)[ED] motif is characteristic of LCL-type C-domains.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QND46664.1	0.0	50	2387	602	2929
ACX49739.1	1.43e-301	11	1774	12	1816
BAZ00088.1	1.18e-236	972	2248	2140	3423
BAZ75991.1	1.18e-236	972	2248	2140	3423
BAY90071.1	2.03e-222	1238	2117	303	1177

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U89_A	0.0	438	1509	3	1070	Crystalstructure of a cross-module fragment from the dimodular NRPS DhbF [Geobacillus sp. Y4.1MC1]
6MFZ_A	3.49e-275	463	2110	209	1790	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
6MFY_A	3.33e-261	463	2042	209	1721	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]
6P1J_A	2.81e-211	1058	2033	4	964	Thestructure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae],6P1J_B The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae]
6N8E_A	2.36e-184	1037	2293	14	1245	Crystalstructure of holo-ObiF1, a five domain nonribosomal peptide synthetase from Burkholderia diffusa [Burkholderia diffusa]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39846	0.0	4	2113	6	2072	Plipastatin synthase subunit B OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsB PE=1 SV=1
Q70LM6	0.0	101	2134	2679	4695	Linear gramicidin synthase subunit B OS=Brevibacillus parabrevis OX=54914 GN=lgrB PE=1 SV=1
Q70LM5	0.0	103	2115	2705	4689	Linear gramicidin synthase subunit C OS=Brevibacillus parabrevis OX=54914 GN=lgrC PE=3 SV=1
P27206	0.0	9	2128	6	2092	Surfactin synthase subunit 1 OS=Bacillus subtilis (strain 168) OX=224308 GN=srfAA PE=1 SV=4
P45745	0.0	1	2390	1	2376	Dimodular nonribosomal peptide synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=dhbF PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000053	0.000002	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001371_01120.