CAZyme Information: MGYG000001373_01832

Basic Information

• Species: Faecalimonas sp000209385
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Faecalimonas; Faecalimonas sp000209385
• CAZyme ID: MGYG000001373_01832
• CAZy Family: GH110
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1849	MGYG000001373_1|CGC16	207234.75	5.6531

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001373	2218229	Isolate	not provided	not provided

• Gene Location: Start: 1867178; End: 1872727 Strand: -

Full Sequence      

MKKNRKRSRIMSLLLIFALLLQPTVSLAKDGEESAKKNNGVVIDVRDFGADPTGVNDSAE
AIWKALEEAKKVSDGGKKEVTLNFPKGEYHIYKDKAQKREYHTSNTNSIENPVKTIGILI
EEQKNLTIEGNGSLFMMHGNMMALAVAKSENITLHNFSWDFAVPTVSEMTVTNMGKSGGR
DYTDFYIPNCFPYEIQGNTILWKSENSPYTNQPYWTEKGIHNSYAVVAYQPDDEMTRNYY
TNQGPFQNAIKIEKLENNQVRVHYAVTRPAMQEKGMVFELASSAVRETAGAFTWESKNVR
AEKVNVHFMHGFGWLIQMSENVYYKDCNLMPRENSGHLTVSYADGIHASGASGEIVIENC
NFANTHDDPINLHGTFTRVEKRIDSHTLELKYIHNQQGGFPQYHVGDKVQFFTRDLLSST
DGEKQYEVAEVISNPGENGNDLRTMKIKFKEDLPENLSDKIGGQPKYVAENVTYAPKVTI
RNCTFRNVPTRGILCTTRKEVIIENNVFHNMSMATIFLSNDSNDWYESGPIRDMKIRNNT
FYIKDIGRTSWEYAPAIYIHPVTKGGQFPDASNPIHKNISIEGNTFYMDEDTVVKAESVE
NLTFKNNKVFRMNPDVTVGITLDNKTIQTGQSVQLKTDAKGNTNNKKVDNVFEFTKSKNI
KIENNVYDDGLKLYAVAEDDATEKNISIKGDDIKVIRNKNQAPAEPVRNICYASTNPDVA
TVDKNGQISGIKSGKTTVFAYYRWNDTIVKSNEIEVTVGGEAAETEKIEIKEEDNKEVKV
NEKVQFTLKKGTGATWSVTDFLTGEETNSATISSTGEFTAKANGVVWVNAVKGTAKDRKA
VVIYGGEVKALNADFEIKREDKAKYKLTDNSIEVTMQPGDLYTNTNTVKNLFLYNPKNVE
KNNLRAVVKVEGLPIKENNQWDTASFILYRDDDNYVTVGKKSHYNGIATVEEKNGAAQEY
HGNEADNNVTSAYLGITKKNDKITLSYQKENGEWQTAKEFTNASFGNDYKIGMACWESNA
RAKKVTFSDFHVGSADTEFNKLLEGEEISVQQTKTVRPTVSDVKTKVENGKAKVEYKFAD
TQGAKEGETVYRWYWKEGEVEKTTVTKEKELSVAGKKDIFCQVYPKDQYGIVGMPSEKVK
VAVSPEQTDELQEISVNNGIVYKRGDNKEQNILVPQSLKKIMLSYVSVNDSVGKTQIYIN
GTEVAGELNNTDTLILEVQDKDELRIVRGKNTYVLTVQLKGESAIQVNKLSMPQLQFEQN
KPIETKSFYMNADGSKATSDIVVETEGKIGKVEVLAGEYRTPLNVSNEGNRYTAKADFRN
GLNSFYVRVYAEDNTTYEQYILNVTYMPSTKMQVNGITLNGKGLTNFDAEKEEYFFALDK
KEKNLNVKVNTDASAVKISLNGEVKNGTEATFNKLKEGENTLLIQCVAGDKLFKKNYRVT
VYKHFDVNTGIQEVILDGKNITSDLLNEKKTSEQFVDSDKTTLKVTAIDPDATVEVKQTR
EKAAKGNVFEGELNVYDGLQNVDIVVTAADRKTKETYRIELRKGAYLSDLEWESATVGYG
TQVNRDRNHLGSTIQLANEKGEPVAFKKGLGTHAESVIVYDITGKGYKALEGFVGIDYCQ
YNADDGKVQFCIEVDGQIVFDSGEMAQKTPMKKFAIELPENAKKVTLKALKGENNFNDHA
DWADAKFLGEFPEKTYTITANANDDKMGVVEIVPEKEVYQIGEEVKVIANVNEGYKFTGW
LENNEIISTEKEYTFTVDKDRELTAQFEKEEKPVEPDKPVVPEKPNKPVAPQIPPKPDRP
NEQKPSQNGNAGQPQKPVKTGDDSQSTLALFGFGISAIALAYVLGRKRK

Enzyme Prediction     

No EC number prediction in MGYG000001373_01832.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH110	42	615	1.5e-157	0.9562043795620438
CBM51	1546	1687	1.5e-35	0.9850746268656716

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam08305	NPCBM	3.71e-36	1546	1687	4	135	NPCBM/NEW2 domain. This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins. This domain has also been called the NEW2 domain (Naumoff DG. Phylogenetic analysis of alpha-galactosidases of the GH27 family. Molecular Biology (Engl Transl). (2004)38:388-399.)
smart00776	NPCBM	1.38e-29	1546	1687	6	144	This novel putative carbohydrate binding module (NPCBM) domain is found at the N-terminus of glycosyl hydrolase family 98 proteins.
pfam13229	Beta_helix	3.41e-05	478	609	11	110	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam13229	Beta_helix	1.18e-04	472	610	28	135	Right handed beta helix region. This region contains a parallel beta helix region that shares some similarity with Pectate lyases.
pfam12733	Cadherin-like	1.27e-04	1475	1542	20	87	Cadherin-like beta sandwich domain. This domain is found in several bacterial, metazoan and chlorophyte algal proteins. A profile-profile comparison recovered the cadherin domain and a comparison of the predicted structure of this domain with the crystal structure of the cadherin showed a congruent seven stranded secondary structure. The domain is widespread in bacteria and seen in the firmicutes, actinobacteria, certain proteobacteria, bacteroides and chlamydiae with an expansion in Clostridium. In contrast, it is limited in its distribution in eukaryotes suggesting that it was derived through lateral transfer from bacteria. In prokaryotes, this domain is widely fused to other domains such as FNIII (Fibronectin Type III), TIG, SLH (S-layer homology), discoidin, cell-wall-binding repeat domain and alpha-amylase-like glycohydrolases. These associations are suggestive of a carbohydrate-binding function for this cadherin-like domain. In animal proteins it is associated with an ATP-grasp domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AYE35188.1	0.0	4	1374	6	1393
QAS60591.1	0.0	4	1374	6	1393
AYV35227.1	5.85e-245	1	1523	1	1445
VEH83785.1	1.27e-244	1	1524	1	1446
AGN25168.1	3.47e-244	1	1524	1	1446

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7JW4_A	5.36e-56	38	607	23	564	Crystalstructure of PdGH110B in complex with D-galactose [Pseudoalteromonas distincta],7JW4_B Crystal structure of PdGH110B in complex with D-galactose [Pseudoalteromonas distincta]
7JWF_A	2.38e-55	38	607	23	564	Crystalstructure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_B Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_C Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta],7JWF_D Crystal structure of PdGH110B D344N in complex with alpha-(1,3)-galactobiose [Pseudoalteromonas distincta]
7JRM_A	4.29e-21	1518	1687	55	223	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]
2VMH_A	4.41e-21	1538	1686	2	149	Thestructure of CBM51 from Clostridium perfringens GH95 [Clostridium perfringens]
7JRL_A	5.19e-21	1518	1687	77	245	ChainA, F5/8 type C domain protein [Clostridium perfringens ATCC 13124]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
B1V8K7	9.55e-116	21	761	10	726	Alpha-1,3-galactosidase A OS=Streptacidiphilus griseoplanus OX=66896 GN=glaA PE=1 SV=1
Q826C5	5.26e-109	34	613	32	587	Alpha-1,3-galactosidase A OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=glaA PE=3 SV=1
Q5L7M8	1.25e-82	45	611	28	552	Alpha-1,3-galactosidase A OS=Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow) OX=272559 GN=glaA PE=3 SV=1
Q64MU6	1.42e-81	45	611	28	552	Alpha-1,3-galactosidase A OS=Bacteroides fragilis (strain YCH46) OX=295405 GN=glaA PE=3 SV=1
Q8A2Z5	1.45e-76	70	615	36	538	Alpha-1,3-galactosidase A OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=glaA PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000286	0.998964	0.000198	0.000195	0.000179	0.000160

TMHMM  Annotations     

start	end
1826	1845