CAZyme Information: MGYG000001376_02348

Basic Information

• Species: Dysgonomonas gadei
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Dysgonomonadaceae; Dysgonomonas; Dysgonomonas gadei
• CAZyme ID: MGYG000001376_02348
• CAZy Family: CE12
• CAZyme Description: Rhamnogalacturonan acetylesterase RhgT

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
259	MGYG000001376_5|CGC6	29192.59	8.2396

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001376	5166013	Isolate	not provided	not provided

• Gene Location: Start: 357244; End: 358023 Strand: +

Full Sequence      

MNKTSVLLIITLCLLSLSAIAQKRIFMIGDSTMADKVLFKTVTDSISGDSICEPFPEKGW
GQLLPTFFNDNVEIKNYAKNGRSSRSFIAEGLWQQVIDNISKDDYLIIQFGHNDAAIEKK
DRYTTPEEYEKNFTMFVEKAKAKGAKPIICTSVVRRRFDKNGVFQDSHGKYLDLARGVAK
KEKIPMIDMYEKSKALITGLGDEDSKPLFLHLKPGENKNFPKGNTDNTHFNEKGAIAIAT
LFVEGLKEQNIEDLVKELK

Enzyme Prediction     

No EC number prediction in MGYG000001376_02348.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
CE12	24	246	1.8e-73	0.9952380952380953

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01821	Rhamnogalacturan_acetylesterase_like	5.18e-85	24	246	2	198	Rhamnogalacturan_acetylesterase_like subgroup of SGNH-hydrolases. Rhamnogalacturan acetylesterase removes acetyl esters from rhamnogalacturonan substrates, and renders them susceptible to degradation by rhamnogalacturonases. Rhamnogalacturonans are highly branched regions in pectic polysaccharides, consisting of repeating -(1,2)-L-Rha-(1,4)-D-GalUA disaccharide units, with many rhamnose residues substituted by neutral oligosaccharides such as arabinans, galactans and arabinogalactans. Extracellular enzymes participating in the degradation of plant cell wall polymers, such as Rhamnogalacturonan acetylesterase, would typically be found in saprophytic and plant pathogenic fungi and bacteria.
COG2755	TesA	3.28e-22	15	254	1	216	Lysophospholipase L1 or related esterase [Amino acid transport and metabolism].
cd00229	SGNH_hydrolase	3.56e-14	25	244	1	186	SGNH_hydrolase, or GDSL_hydrolase, is a diverse family of lipases and esterases. The tertiary fold of the enzyme is substantially different from that of the alpha/beta hydrolase family and unique among all known hydrolases; its active site closely resembles the typical Ser-His-Asp(Glu) triad from other serine hydrolases, but may lack the carboxlic acid.
pfam13472	Lipase_GDSL_2	7.15e-13	48	234	3	173	GDSL-like Lipase/Acylhydrolase family. This family of presumed lipases and related enzymes are similar to pfam00657.
pfam00657	Lipase_GDSL	1.34e-09	48	239	5	221	GDSL-like Lipase/Acylhydrolase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QIK58725.1	1.77e-109	9	259	11	261
QIK53308.1	3.37e-107	9	259	11	261
QRX62572.1	2.60e-79	3	259	2	259
SCM55356.1	3.05e-72	27	259	1	232
AHM58505.1	4.30e-69	24	251	29	242

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1DEO_A	2.51e-12	25	246	3	212	RHAMNOGALACTURONANACETYLESTERASE FROM ASPERGILLUS ACULEATUS AT 1.55 A RESOLUTION WITH SO4 IN THE ACTIVE SITE [Aspergillus aculeatus],1DEX_A RHAMNOGALACTURONAN ACETYLESTERASE FROM ASPERGILLUS ACULEATUS AT 1.9 A RESOLUTION [Aspergillus aculeatus],1K7C_A Rhamnogalacturonan acetylesterase with seven N-linked carbohydrate residues distributed at two N-glycosylation sites refined at 1.12 A resolution [Aspergillus aculeatus],1PP4_A The crystal structure of rhamnogalacturonan acetylesterase in space group P3121 [Aspergillus aculeatus],1PP4_B The crystal structure of rhamnogalacturonan acetylesterase in space group P3121 [Aspergillus aculeatus]
3C1U_A	1.21e-11	25	246	3	212	ChainA, Rhamnogalacturonan acetylesterase [Aspergillus aculeatus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31523	5.32e-55	24	248	7	214	Rhamnogalacturonan acetylesterase RhgT OS=Bacillus subtilis (strain 168) OX=224308 GN=rhgT PE=1 SV=1
O31528	6.56e-37	46	257	7	216	Probable rhamnogalacturonan acetylesterase YesY OS=Bacillus subtilis (strain 168) OX=224308 GN=yesY PE=1 SV=1
Q5BAA2	6.78e-13	18	246	14	224	Rhamnogalacturonan acetylesterase OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=AN2528 PE=1 SV=1
Q00017	1.23e-11	18	246	14	229	Rhamnogalacturonan acetylesterase OS=Aspergillus aculeatus OX=5053 GN=rha1 PE=1 SV=1
P54451	1.05e-08	60	223	49	227	Uncharacterized lipoprotein YqeF OS=Bacillus subtilis (strain 168) OX=224308 GN=yqeF PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000261	0.999127	0.000157	0.000146	0.000130	0.000127

TMHMM  Annotations     

start	end
5	22