CAZyme Information: MGYG000001378_00300

Basic Information

• Species: Bacteroides ovatus
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Bacteroides; Bacteroides ovatus
• CAZyme ID: MGYG000001378_00300
• CAZy Family: GH2
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
988	MGYG000001378_1|CGC11	113205.09	7.0586

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001378	6545242	Isolate	not provided	not provided

• Gene Location: Start: 442227; End: 445193 Strand: -

Full Sequence      

MMKRILVITFILCVFSLSEGCAAGREKYNFNSDWLLKIGDVPNAQAVGFEDSDWNMVTLP
RAFNENESFKVAIDQLTDTIVWYRKHFKVAVLDARKVFVEFEGVRQGADFYLNGYHLGKH
ENGVMAVGFDLTPYMKNGDNVMAVRVDNDWSYKEVSTNTKFQWHDNRFNCNYGGIPKNVF
LHVTNDVYQTLPLYNNLKTTGLYVYATDFDIKNRKAKIHVESEVKNDSHKSRKFSFLVTL
VDADGKNVKVFKGPDVCLKSKEVKIIKAEAEVNNLHFWSWGYGYLYTVKTKLVNADNEVV
DEVNTRTGFRKTKFGEGKVWLNDRVIHLKGYAQRTTNEWPGVGMSVPAWLSDYSNDLVVK
SNANLVRWMHVTPWKQDVESCDRVGLIQAMPAGDQERDSKGRQWEQRTELMRDAIIYNRN
NPSILFYECGNKGIKREHMLEMKAIRDKYDPYGGRAIGSREMLDIREAEYGGEMLYVNKS
RHHPMWSMEYCRDEGLRKYWDDYSYPYHKEGDGPLHRGGSAKSYNHNQDMFAVELVRRWY
DYWRERPGTGLRINAGGAKIIFSDSNTHFRGAENYRRSGVTDAMRIEKDGFFAHQVMWNG
WVDIESEQTYIIGHWNYPENTVKPVYVVSTGEKVELFLNDRLLGEGEREYDFLFTFDKVA
FKPGKLEAVSYNKAGKELSRYVLTTVGEPTNIRLTAIQNPEGFHADGADMALIQFEVVDK
DGKRCPLDNRIVKFDLKGEAEWRGGIAQGEDNYILSKDLPVECGVNRVLIRSTTKAGKLI
LTAKAKGLPEASITLETIPVEKVDGVSNYLPQMTLKGRLDKGETPLSSSYRDKKVNIGIV
SAKAGANSQNVAKSYDDDELSEWTNDGQLSTAWITYQLEREANIDDVCLKLRGWRKNSYP
LEVYAGNTLVWSGKTERNLGYIHLKTMPMRTDNITIRLKGSVSVKDAFGEIVEVEDKASN
TRVQEKADEERYLPIIEVEFLENSKLNF

Enzyme Prediction     

EC	3.2.1.31

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH2	24	545	1.6e-58	0.5824468085106383

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG3250	LacZ	5.05e-23	29	512	29	495	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
pfam18565	Glyco_hydro2_C5	1.57e-18	692	794	1	103	Glycoside hydrolase family 2 C-terminal domain 5. Domain 5 is found in dimeric beta-D-galactosidase from Paracoccus sp. 32d, which contributes to stabilization of the functional dimer. It is suggested that the location of this domain 5, may be one of the factors responsible for the creation of a functional dimer and cold-adaptation of this enzyme.
PRK10150	PRK10150	2.76e-18	25	431	10	416	beta-D-glucuronidase; Provisional
PRK10340	ebgA	3.13e-16	83	431	113	448	cryptic beta-D-galactosidase subunit alpha; Reviewed
pfam00703	Glyco_hydro_2	5.02e-14	204	310	1	106	Glycosyl hydrolases family 2. This family contains beta-galactosidase, beta-mannosidase and beta-glucuronidase activities.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGT72520.1	0.0	1	988	1	988
QGT72522.1	0.0	1	985	1	987
ALJ49030.1	0.0	1	985	1	987
QRQ55837.1	0.0	1	985	1	987
SCV08447.1	0.0	1	985	1	987

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6HPD_A	2.47e-151	25	813	35	820	Thestructure of a beta-glucuronidase from glycoside hydrolase family 2 [Formosa agariphila KMM 3901]
5T98_A	1.78e-52	22	795	22	818	Crystalstructure of BuGH2Awt [Bacteroides uniformis],5T98_B Crystal structure of BuGH2Awt [Bacteroides uniformis],5T99_A Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis],5T99_B Crystal structure of BuGH2Awt in complex with Galactoisofagomine [Bacteroides uniformis]
7CWD_A	1.30e-51	26	797	2	801	ChainA, beta-glalactosidase [Niallia circulans],7CWI_A Chain A, beta-galactosidase [Niallia circulans]
4YPJ_A	1.79e-49	24	798	6	808	ChainA, Beta galactosidase [Niallia circulans],4YPJ_B Chain B, Beta galactosidase [Niallia circulans]
6B6L_A	8.10e-46	23	795	3	775	Thecrystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838],6B6L_B The crystal structure of glycosyl hydrolase family 2 (GH2) member from Bacteroides cellulosilyticus DSM 14838 [Bacteroides cellulosilyticus DSM 14838]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
T2KN75	1.08e-150	25	813	26	811	Beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22060 PE=1 SV=1
A7LXS9	1.33e-49	18	795	35	846	Beta-galactosidase BoGH2A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02645 PE=1 SV=1
P77989	7.23e-48	24	792	2	732	Beta-galactosidase OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=lacZ PE=3 SV=2
T2KM09	1.94e-39	11	741	34	761	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_22050 PE=2 SV=2
T2KPJ7	1.59e-32	2	725	27	757	Putative beta-glucuronidase OS=Formosa agariphila (strain DSM 15362 / KCTC 12365 / LMG 23005 / KMM 3901 / M-2Alg 35-1) OX=1347342 GN=BN863_21970 PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000447	0.095478	0.903975	0.000044	0.000051	0.000043

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001378_00300.