CAZyme Information: MGYG000001380_03014

Basic Information

• Species: Ruminococcus_B gnavus
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Ruminococcus_B; Ruminococcus_B gnavus
• CAZyme ID: MGYG000001380_03014
• CAZy Family: CBM48
• CAZyme Description: 1,4-alpha-glucan branching enzyme GlgB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
641	MGYG000001380_12|CGC1	74561.32	6.5406

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001380	3437962	Isolate	not provided	not provided

• Gene Location: Start: 79694; End: 81619 Strand: -

Full Sequence      

MGRQKTVQKKLQPYEIGELDQYLFGQGTHYEIFRKMGAHKAVQKKQEGVYFAVWAPHAAR
VSVVGEFNAWDFGANEMHRQEPLGIYTCFVPGAQEGDLYKFCIETQTGERIFKADPFANY
AERRPGTASRVTDISNLKWSDEQWLQKRKKWDQKENPLAIYEVHMGSWMRHPGTEDEGFY
TYREFAHSITDYVKKMGYTHVELMGIAEHPFDGSWGYQVTGYYAPTSRYGTPQDFAYMIN
YLHRNGIGVILDWVPAHFPKDAHGLADFDGTPTFEYADPRKGEHPDWGTKIFDYSKPEVQ
NFLIANALFWIEHYHVDGLRVDAVASMLYLDYGKQDGQWVPNQYGGNQNLEAIAFFKHLN
SVVLGRNQGAMMIAEESTAWPKVTGAPEDDGLGFSLKWNMGWMHDFTEYMKLDPLFRKGA
HYQMTFSMEYAYSENYILVLSHDEVVHLKCSMLNKMPGLGFDKFANLKAAYAFMTGHPGK
KLLFMGQEFAQLREWSEERELDWYLLAEEPHQQIQNWYRDLLHLYRHNKALYELDTDPAG
FEWINKDDTFRSIFSFVRHSRDNKKNLLFVCNFTPMERLDYRVGVPRRKQLKLVLDSDDP
KYGGNGVERPKVYKPVKQECDGQKYSIAYPLQPYGVAVFEF

Enzyme Prediction     

EC	2.4.1.18

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH13	191	491	1.3e-152	0.9966777408637874
CBM48	35	120	4.8e-16	0.8947368421052632

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK12313	PRK12313	0.0	18	641	9	627	1,4-alpha-glucan branching protein GlgB.
PRK14706	PRK14706	0.0	36	641	27	621	glycogen branching enzyme; Provisional
PRK14705	PRK14705	0.0	12	639	595	1220	glycogen branching enzyme; Provisional
COG0296	GlgB	0.0	16	640	6	627	1,4-alpha-glucan branching enzyme [Carbohydrate transport and metabolism].
PRK05402	PRK05402	0.0	16	640	100	722	1,4-alpha-glucan branching protein GlgB.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRT30382.1	0.0	1	641	1	641
QHB24149.1	0.0	1	641	1	641
QEI31650.1	0.0	1	641	1	641
CBL27570.1	0.0	1	641	1	641
QUO31484.1	0.0	5	641	3	637

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5GQW_A	2.28e-223	13	640	127	772	Crystalstructure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQX_A Crystal structure of branching enzyme W610N mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR2_A	3.22e-223	13	640	127	772	Crystalstructure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GR4_A Crystal structure of branching enzyme L541A mutant from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]
5GR5_A	3.22e-223	13	640	127	772	Crystalstructure of branching enzyme W610A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQZ_A	4.56e-223	13	640	127	772	Crystalstructure of branching enzyme Y500A mutant from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142]
5GQU_A	6.46e-223	13	640	127	772	Crystalstructure of branching enzyme from Cyanothece sp. ATCC 51142 [Crocosphaera subtropica ATCC 51142],5GQV_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltohexaose [Crocosphaera subtropica ATCC 51142],5GQY_A Crystal structure of branching enzyme from Cyanothece sp. ATCC 51142 in complex with maltoheptaose [Crocosphaera subtropica ATCC 51142]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P30539	1.59e-304	16	641	7	632	1,4-alpha-glucan branching enzyme GlgB OS=Butyrivibrio fibrisolvens OX=831 GN=glgB PE=1 SV=1
Q7NL20	2.26e-239	16	640	103	727	1,4-alpha-glucan branching enzyme GlgB OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=glgB PE=3 SV=1
Q8DLB8	6.89e-239	15	640	105	749	1,4-alpha-glucan branching enzyme GlgB OS=Thermosynechococcus vestitus (strain IAM M-273 / NIES-2133 / BP-1) OX=197221 GN=glgB PE=3 SV=1
B3PGN4	1.20e-237	15	630	114	727	1,4-alpha-glucan branching enzyme GlgB OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=glgB PE=3 SV=1
B8CVY1	6.50e-236	18	640	8	626	1,4-alpha-glucan branching enzyme GlgB OS=Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562) OX=373903 GN=glgB PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000045	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001380_03014.