CAZyme Information: MGYG000001380_03044

Basic Information

• Species: Ruminococcus_B gnavus
• Lineage: Bacteria; Firmicutes_A; Clostridia; Lachnospirales; Lachnospiraceae; Ruminococcus_B; Ruminococcus_B gnavus
• CAZyme ID: MGYG000001380_03044
• CAZy Family: GH3
• CAZyme Description: Thermostable beta-glucosidase B

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
764	MGYG000001380_13|CGC1	85265.48	4.5279

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001380	3437962	Isolate	not provided	not provided

• Gene Location: Start: 15877; End: 18171 Strand: -

Full Sequence      

MEKEKIQNLISQMTLEEKAGMCSGADFWHLKGIERLGIPSVMVTDGPHGLRKQAQGADHL
GINESEKAICFPAGCATASSFDRDLIRRQGELLGQECQAMDVSTILGPAMNIKRSPLCGR
NFEYYSEDPFVSTEMAAALIEGIQSKNVGTSAKHFVANNQEKRRMTNSSDMDERTLREIY
LASFEGAVKKAKPWTVMSSYNRVNGEFVGDSKEYLTEILREEWGFDGYVVSDWGAVNDRV
SSLAAGLDLEMPPGDFENDRLIVKAVREGKLDESVVDQACERVLDVIFRYTENRDTEAVF
DYEKDHEAAAEIEAECMVLLKNENEILPLTSDQKIAFIGKYAKTPRYQGGGSSHINSWKV
ESALEAAKEIPELANVTFAEGYQDEKDEVIEELQNEAVKAAAKADVTVLFLGLPDNFESE
GYDRKHMNLPDCQNELVQKVLEVQKHVVVVLHNGSAVVMPWKDQVEGILEAYLGGEAVGK
AVAEVLAGRKNPSGRLAETFPLRLEDTPCYLTYGKGFDNAVYQEGVFVGYRYYTSRKMET
AFPFGYGLSYTTFAYSDLQLDKKEMSDKEHVEVSVKVKNIGKRAGKTVVQLYVAAPEAEV
VRPVRELRGFEKVSLEAGEEKTVTFTLDERAFSYWNTQIHDWYAEEGSYQVMIGENADQM
CLSGEILVHPTKEIPKVYSLNTCLGELMRDPKAQAVMAPFMQEMAQNDAATDMAEANAND
QSGVVNQEMMAAMMEGMPLRQLLSFVPGIKREMLEQMVNALNQL

Enzyme Prediction     

No EC number prediction in MGYG000001380_03044.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH3	32	252	1.3e-69	0.9861111111111112

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
PRK15098	PRK15098	9.27e-102	6	654	38	751	beta-glucosidase BglX.
COG1472	BglX	2.10e-70	26	356	48	374	Periplasmic beta-glucosidase and related glycosidases [Carbohydrate transport and metabolism].
pfam01915	Glyco_hydro_3_C	9.15e-57	317	530	1	216	Glycosyl hydrolase family 3 C-terminal domain. This domain is involved in catalysis and may be involved in binding beta-glucan. This domain is found associated with pfam00933.
PLN03080	PLN03080	3.11e-54	3	638	50	754	Probable beta-xylosidase; Provisional
pfam00933	Glyco_hydro_3	6.52e-42	35	286	63	316	Glycosyl hydrolase family 3 N terminal domain.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QRT30267.1	0.0	1	764	1	764
QHQ63232.1	0.0	4	763	4	755
QUI21624.1	1.40e-305	6	762	2	754
QYH55286.1	4.15e-304	1	763	1	754
AHF25022.1	2.01e-302	1	763	1	765

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
7MS2_A	6.25e-285	6	764	5	754	ChainA, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2],7MS2_B Chain B, Thermostable beta-glucosidase B [Acetivibrio thermocellus AD2]
5WAB_A	7.88e-189	10	742	9	724	CrystalStructure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_B Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_C Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703],5WAB_D Crystal Structure of Bifidobacterium adolescentis GH3 beta-glucosidase [Bifidobacterium adolescentis ATCC 15703]
2X40_A	3.15e-137	4	668	2	713	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with glycerol [Thermotoga neapolitana DSM 4359],2X41_A Structure of beta-glucosidase 3B from Thermotoga neapolitana in complex with glucose [Thermotoga neapolitana DSM 4359]
2X42_A	4.85e-136	4	668	2	713	Structureof beta-glucosidase 3B from Thermotoga neapolitana in complex with alpha-D-glucose [Thermotoga neapolitana DSM 4359]
3AC0_A	8.27e-128	1	660	1	828	Crystalstructure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_B Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_C Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus],3AC0_D Crystal structure of Beta-glucosidase from Kluyveromyces marxianus in complex with glucose [Kluyveromyces marxianus]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P14002	4.85e-284	6	764	5	754	Thermostable beta-glucosidase B OS=Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=bglB PE=1 SV=2
F6C6C1	8.30e-181	10	698	9	701	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium breve (strain ACS-071-V-Sch8b) OX=866777 GN=HMPREF9228_1477 PE=1 SV=1
E7CY69	3.30e-180	10	698	9	701	Exo-alpha-(1->6)-L-arabinopyranosidase OS=Bifidobacterium longum OX=216816 GN=apy PE=1 SV=1
A7LXS8	3.29e-133	5	660	45	737	Beta-glucosidase BoGH3A OS=Bacteroides ovatus (strain ATCC 8483 / DSM 1896 / JCM 5824 / BCRC 10623 / CCUG 4943 / NCTC 11153) OX=411476 GN=BACOVA_02644 PE=1 SV=1
Q5BB53	2.25e-130	6	672	6	834	Probable beta-glucosidase I OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=bglI PE=2 SV=2

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000045	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001380_03044.