CAZyme Information: MGYG000001391_02716

Basic Information

• Species: Coprobacter fastidiosus
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Coprobacteraceae; Coprobacter; Coprobacter fastidiosus
• CAZyme ID: MGYG000001391_02716
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1603	MGYG000001391_5|CGC8	177763.52	4.8093

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001391	3843461	Isolate	not provided	not provided

• Gene Location: Start: 345601; End: 350412 Strand: -

Full Sequence      

MNIKKLFITLFVILGVGSAIAQQPYSPCWHPDNIKNWKPSDDQDAKFNRSRVPLQPRFWD
NTVKANSSQFADSRVAACLTMNPECSLTPSQDADNILGYNPTYWQYMDLLIWWGGSAGEG
IIIPPSAPVVDVAHMNGVKVLGQLFFPPRAFSGDPAWLRQMLTKEGGEYPYARKLYEIAD
YYGFDGWFINEELHGALPGDWMGFIETFNKCKKAGGKEYMEIQWYNNDIVVGDYMDMVKM
DGVSYFLDYGSNTVSNIQSQMQAVKNAGMADIAFSKLYFGIESAINGFTGNADKFSNLFT
STGHRGSPNIFNPEEGIWKKVVDEFRKNPVNGSAEQYKKMQEVFQNESRFWTNAKGDPSD
NSAWGSSTWPGLANTILERSVIQTKPFVTTFGAGLGKKRFVNGEEKGNHDWYHRGMQTIM
PTWRWWFDNKTLSADIYLDDAYNMGTSVKVSGALSADADHIMRLYKTRLAIASGDKIQLV
YKGSGAVIEAKLGISENANEFATVTLNTVSTNNGWTVAEGDLSSLEGKTVSIIALNFRSS
SNLSSYEVLLGQLAVYPQGYYPELARVENLKIENKLAANSGDIRVVWDAPKSEDIDHYNI
YLQRNGVKSLVGQTRDEGFYIPKFNRSSDSETGVTVSVTAVVKDMREGAEVSASVNYRDL
AAPEVVLSASQTLIPVNGTVTLYAKTEDNPTGYEWILPSSARKTGNGGQISVQFTQAGVF
DIGVKVTNKAGTTEITEKAFVEVANNADDLKVVSVDKQIHSVSGQLAGEEAYHLIDGVTK
NFSDVHQKWCIGGKKEHWVVVDLQESYKLYKFRIIDCKQGEPEFDNISMYKIYVSNDAVD
WTLIADERSNPATEKTIWVKPTIARYVKFVPYSTEPVTIRIWEFEAYGVATKLTLDKSEN
LLLGLGESAEKEFSYGFGGTEKEENFNIEVKTDRPDLLEIKNIRHDEVKNKISFDMLSLE
IGTAEVEVSIKNGDWVKSSVFEVKIEDLSLINIARGALPSIVMKDAMSAILNEPNLKPEN
ALTDGNRSTFWAGWKTFSVKIDLGKIYDARVIKFMFEPADYNVVGNRCYFPSEVKVYTAQ
TDEESAYASVAWFENKNLQEINEVVLPVDGRQVRYVRLEVTGYDERRVFLQEIEVLAKTD
KENPLLIGNVNGVKIEVGKSVKKKIPVDLGDMSVEDNLEVVSRLKSVSGSDVAIVNASYD
EDARKILLDIEGKTPGNADLVVALKNGDWQVCTNLSVVVFSGGNLALGKSITESHVSATG
TPLSGGGKADVKTLVDGSKPIKGDGSADFNNLWCGPSWQNNTKSTVVIDLESEASISDIK
VFGAVFWSTVSVPGEVKIFIGSEDSESSYQEVYTWKRSSEYETSNKYRHMEFDYHLPSPM
TGRYVKLQLTQNNEYGMAVALREIEVYGESSSDGETPSNTLSVSPIEAVTLNGGESKTLE
TSYSLGGASQKENFDISVSAQDGNIVKVSKVMYDEVQQKITFELRGLKKGTTGVRIVFEN
GKWSTSSDVNVSVLSDDPLLGIENVKTVACKVYPNPVRKGGVLVIEAEQIRMVKILTLQG
SLLREVAVESDRCELNMGYLSSGVYLLIIDTENDMKVQKVTVK

Enzyme Prediction     

No EC number prediction in MGYG000001391_02716.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	85	398	2.6e-50	0.9746031746031746

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	8.08e-104	1	555	1	549	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	3.62e-57	99	430	25	338	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	1.01e-52	98	396	7	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
pfam00754	F5_F8_type_C	5.41e-12	758	871	1	112	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
cd00057	FA58C	3.24e-06	755	888	10	143	Substituted updates: Jan 31, 2002

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QUT77959.1	3.27e-254	10	1041	2	1055
EFC71183.2	6.11e-244	3	1141	40	1192
QZE14770.1	3.77e-240	4	1322	3	1285
EFC71182.2	1.57e-239	8	996	9	1003
QUB46808.1	4.13e-235	1	996	1	1005

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3FHA_A	5.63e-84	24	621	6	583	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHQ_A	2.59e-83	24	621	6	583	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
2VTF_A	3.97e-83	24	621	11	588	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]
2W91_A	7.75e-60	32	644	18	621	Structureof a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D. [Streptococcus pneumoniae TIGR4],2W92_A Structure of a Streptococcus pneumoniae family 85 glycoside hydrolase, Endo-D, in complex with NAG-thiazoline. [Streptococcus pneumoniae TIGR4]
3GDB_A	3.61e-58	32	644	169	772	Crystalstructure of Spr0440 glycoside hydrolase domain, Endo-D from Streptococcus pneumoniae R6 [Streptococcus pneumoniae R6]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
F4JZC2	1.30e-13	23	558	17	540	Cytosolic endo-beta-N-acetylglucosaminidase 1 OS=Arabidopsis thaliana OX=3702 GN=ENGASE1 PE=1 SV=1
Q8BX80	3.70e-12	99	486	142	496	Cytosolic endo-beta-N-acetylglucosaminidase OS=Mus musculus OX=10090 GN=Engase PE=1 SV=1
Q8NFI3	7.50e-11	103	485	154	503	Cytosolic endo-beta-N-acetylglucosaminidase OS=Homo sapiens OX=9606 GN=ENGASE PE=1 SV=1
Q9SRL4	8.40e-10	33	609	36	626	Cytosolic endo-beta-N-acetylglucosaminidase 2 OS=Arabidopsis thaliana OX=3702 GN=ENGASE2 PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000249	0.999144	0.000157	0.000140	0.000124	0.000122

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001391_02716.