CAZyme Information: MGYG000001405_02467

Basic Information

• Species: Ferdinandcohnia timonensis
• Lineage: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae_L; Ferdinandcohnia; Ferdinandcohnia timonensis
• CAZyme ID: MGYG000001405_02467
• CAZy Family: CBM50
• CAZyme Description: Cell division suppressor protein YneA

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1848		198495.79	4.5305

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001405	4608762	Isolate	not provided	not provided

• Gene Location: Start: 827495; End: 833041 Strand: -

Full Sequence      

MKRKKYIVTDGAPDHLSRVFFVWALMGENLNKGMNKKEVEKLRRTLNIEVFCLEIQGYKL
VKNKDDYTLVIYVESQMTEFASEFGGHFYREKKDLNTQIRHLVKEKFPHIPIRAAKVLAG
SMLITTFYLGATGPNVGAQTISGQTQTVNDMYTVQAGDTLYSIAKRYNVSVDSIKKVNGL
TSDMLFVGQQLRLPFFTYTVSSGDTLYSIAKRYNTTVDQLRTTNQLKTDMLSIGQKLRIP
QLQATSTTTTQPTQGTVTEQPTTTQPVEETATAPPPTTAQPAEETVTAPAPTPVQETPTP
APTTSTYTVVAGDTLYGISMKFGTTVDQLKSLNNLTTTVLNVGQVLKVPGTTGSDVAAPT
APATTTYTVVAGDTLYSISQKFGTTVSVIQAANTLTTTNLSIGQVLTIPAEGTVTAPPPT
EQPTPVETPADTTAPIAPTFAPIPLVSTANQSNLNVTGTTEANALVTLTITDQTNAPKTI
QVKANQNGTFGTKIDVSNLQDGSLTITATATDEAGNKSVVATQTIKKDTKTDAPNIINTQ
IMNRQSATAYPVKGTAEPASRVQIVFFDGVNAPITTEVLANEKGEFSAIVNLGKLRDGPI
SITTKAIDSSGNESGMTKLTVKKDTTVEAPVFQELQDITGKNVAEFTLVGNAEPNATITI
RATDGANNRVDTKITTNENGAFRSALNLSGLKDGNITLTAITTDEAQNSSDPQQVTITKD
TFIAEPVLDTKQNITTQNANSYTLRGIADSGSTVQIVLSDGVNPEMTVEAVANENGEFTT
NVDLRNLNDGEIFITASAMDEHGNRSEVEQATLMKETTLPPPVISNTEMINSKTAESYHI
FGIAQPGTTVDITVSDGVNPDIITSTTANQNGEYNVAVDASALLDTSLTISAFQTSAAGI
TSNVSNTTVEKDTKAPTAPIFNNDEFITGTNQANYELFGKAEANTNIQIRIFNGNGQERT
VTATTNENGEYRVPVDLTSFSDGDLSFELTQVDRAGNVSPAVTKTLIKDTVGPAAIQLET
PLPIFSDNVLMYPIRGVGEPHATVVVEISDGVSVVTKNIETDDNGRFEEIFDLSTLRDGE
LTATFRATDNAGNVGESQEFILEKDTAAPAGVVTNQPSYVNTTNQSNFTITGSSVEEGAT
VEMVISDGTTTVRQTAQVVNGAFGANFNLSGLKDGPLTFEARQTDVAGNKSIAEATTIEK
DTVVENAVATKNGFRYENLQYIYTVIGTAEANAKVEVSLFDANKNVLVTRIANADKNGFY
SVDVYVDKASNVASASVRQTDTAGNTSEVTTVSMSSYTVAQGDSLYAVAKRYNTTVDSLM
SLNNMTSDVIQPNQTLRLPITASEVVNLGYMYFGNTSQYVNTVNSTGHAVNIVSPSYFDI
NTDGTLKLTYQVDPKFIETMHRQGIRVVPFLSNHWNRDVGRAMLANKELAAQQIADAIAR
YNLDGVNVDIENVTDADRANYTEFVRLLREKIPPTKEVSVAVAANPNGWATGWHGSYDYT
NLAKYADYLMIMAYDESYQGGDPGSVASYSWVEKSIQYALAQNVAPDKVVLGIAHFGRYW
MEGQSYGGYGISNTQVQTLIDRYNGTVVFDEVSKTPKATITIKAGDPLTFVNGAALSPGT
YTIWFENDESIQKKLELVGKYNIRGVGNWSLGQENKSVWNSYTTTLPNTVPVVSPIYTGP
VQTYKTYTVVAGDTLWVIANRNDTTVSELKEANGMTTDSLFVGQVLRIPTDEAENVVTPT
PAPPATETAAPVYTVNYTVVGGDTLSAIAAKNGTTVTAIKTANNLTSDMIYVGQVLKVPT
NVVIHTVASGDTLYGIAKRYNTTVTAIKTANNLTSDILSIGQTLKIPV

Enzyme Prediction     

No EC number prediction in MGYG000001405_02467.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH18	1352	1657	1.8e-41	0.9290540540540541
CBM50	152	194	1.9e-16	0.975

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd02874	GH18_CFLE_spore_hydrolase	1.10e-71	1345	1662	2	313	Cortical fragment-lytic enzyme (CFLE) is a peptidoglycan hydrolase involved in bacterial endospore germination. CFLE is expressed as an inactive preprotein (called SleB) in the forespore compartment of sporulating cells. SleB translocates across the forespore inner membrane and is deposited as a mature enzyme in the cortex layer of the spore. As part of a sensory mechanism capable of initiating germination, CFLE degrades a spore-specific peptidoglycan constituent called muramic-acid delta-lactam that comprises the outer cortex. CFLE has a C-terminal glycosyl hydrolase family 18 (GH18) catalytic domain as well as two N-terminal LysM peptidoglycan-binding domains. In addition to SleB, this family includes YaaH, YdhD, and YvbX from Bacillus subtilis.
COG3858	YaaH	1.39e-70	1297	1659	52	415	Spore germination protein YaaH [Cell cycle control, cell division, chromosome partitioning].
PRK06347	PRK06347	1.01e-30	1657	1846	378	591	1,4-beta-N-acetylmuramoylhydrolase.
pfam00704	Glyco_hydro_18	7.02e-30	1440	1653	102	306	Glycosyl hydrolases family 18.
PRK06347	PRK06347	1.99e-27	1678	1846	324	523	1,4-beta-N-acetylmuramoylhydrolase.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QNF29662.1	0.0	53	1800	1	1675
AST91884.1	5.01e-295	55	1729	4	1488
QOR65000.1	2.84e-284	53	1672	1	1565
ADU32435.1	2.87e-261	55	1729	2	1328
AGK53922.1	8.35e-199	909	1671	356	1131

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3CZ8_A	1.57e-21	1320	1651	1	308	ChainA, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168],3CZ8_B Chain B, Putative sporulation-specific glycosylase ydhD [Bacillus subtilis subsp. subtilis str. 168]
5JH8_A	1.23e-20	1412	1659	73	307	Crystalstructure of chitinase from Chromobacterium violaceum ATCC 12472 [Chromobacterium violaceum ATCC 12472]
4S3K_A	3.36e-20	1348	1659	110	420	ChainA, Spore germination protein YaaH [Priestia megaterium QM B1551]
4S3J_A	5.80e-17	1378	1659	141	418	Crystalstructure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_B Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876],4S3J_C Crystal structure of the Bacillus cereus spore cortex-lytic enzyme SleL [Bacillus cereus ATCC 10876]
6XI3_AAA	3.70e-16	447	804	39	401	ChainAAA, Large adhesion protein (Lap) involved in biofilm formation [Marinobacter nauticus ATCC 49840]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31682	2.57e-72	1348	1565	4	217	Putative glycosylase YkvQ OS=Bacillus subtilis (strain 168) OX=224308 GN=ykvQ PE=3 SV=1
O32258	2.77e-25	1354	1661	37	338	Uncharacterized glycosylase YvbX OS=Bacillus subtilis (strain 168) OX=224308 GN=yvbX PE=3 SV=1
O05495	1.10e-21	1299	1651	6	400	Putative sporulation-specific glycosylase YdhD OS=Bacillus subtilis (strain 168) OX=224308 GN=ydhD PE=1 SV=2
P37531	3.07e-20	1378	1659	139	416	Cortical fragment-lytic enzyme OS=Bacillus subtilis (strain 168) OX=224308 GN=sleL PE=1 SV=2
C0H404	1.02e-19	1579	1661	2	78	Probably inactive glycosylase YkzR OS=Bacillus subtilis (strain 168) OX=224308 GN=ykzR PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000050	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001405_02467.