CAZyme Information: MGYG000001420_00339

Basic Information

• Species: Alistipes senegalensis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; Alistipes senegalensis
• CAZyme ID: MGYG000001420_00339
• CAZy Family: GH28
• CAZyme Description: Beta-galactosidase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1278		143376.01	6.3821

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001420	3985129	Isolate	not provided	not provided

• Gene Location: Start: 444769; End: 448605 Strand: +

Full Sequence      

MTLKRILILGLLLTAAWSAGARNRAGEIERQIRPVTFPDRDFRITDFGAEAGGEADCRPA
VTAAIDRCSREGGGRVVIPAGRWFSKGPVVLKSHVNLHLEAGAVLFFSSDEADYLPAVLT
RWEGTEVYNYSPLIYAWQATNIAVTGQGVIDGRGSHNFAQWKPRQKADQKALRRMGTNLT
PVSDRLFGEGHYLRPAMLEPVNCTDVLIEGVTFVDSPFWVIHPLACENVTVRGVTVDSYN
LNNDGCDPESCTNVLIEDCTFRTGDDGIAIKSGRDNDAWRIGRPTENVLIRNCSFRSKAN
GVCIGSEISGGVRNVVVENVRMSDVGNGIYFKSNLDRGGYIEDVFVCGVEADSVRKTLVL
FEPDYKSESRENHPTAFRGFVIEDVRARWAGKAGIDIRGFADMPVRYVTISRLTLASTPE
PVIVRNAAGIILSEVTINGERQDRRDPDACISLNGKWEVAIADTMPQTYAAKVPVPGVIT
MATPSLGEDLDAVDNREIGYDYVWYRRTFTLDGTAYPRARLRLRAKYNAQVFLNGREIGY
DPHTTYSHGTFDLSEAIDYDGPNELVVRVGSWNTATTPSKENSAEWWRNSRAPGIWDDVT
LCLGQSVSIDHLKILPEVSEGRVRCMATLVNTSTRDERLAIEAAVCDDMLAVSRAATEVS
VPAGGSTKCELMLPADMLRTWSAGKEGTPRLYRMEVTATNTAGVLCSRKSERFGYRSIET
RGRDVLVNGEKVLFRAENIAFVRALNRWADVMFDKAWIRRFLRAAVQEYNFNYLRIHLGH
AYGKWYDIADEEGIMIQDEWRFMHDDEPTGKDLEDTETEFRRWISENVNHPSIVAWDQEN
EGNVRLEALKAELRRYDPTRLWGEDDYVAKHIYEYSENVVKDFSGYTISDTKPSTVLESC
RLWTNEFGLLEPRENFKTSRTASGWGLYYYDRDDIARLLSDLHADIGTFYRSRRLQAWAP
FALLSGAVNGHNFYRGDIADSLYPQPNLLVLKHLNEPVGASVEMNQAREWYKDKTLYRPG
AEYRKTVWVWNDLPRACDVRLRVMLVAPDGTEHICADENVTVAASSAVKREVRLTMPRTQ
GICLVEPRLTLPGGREVAGVRRRLMVARNASKVAERMAFGGRRTPVAGGWSILEHFTGSD
IPDPVRQGIIAAAGDGLIDRIDVSGTPEAPTYIVQSTRYESHDRLHLTTTTFDAEGRVRD
IRRAEAMPYVNLPAPVRRTIVEVVGSVPVDESRVIRRGEGDETTYEITMIGSDLRYKLRI
TDDGVLHDKKVTRKKAKK

Enzyme Prediction     

No EC number prediction in MGYG000001420_00339.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH28	63	418	3.8e-75	0.9323076923076923
GH2	440	1006	1.3e-65	0.6289893617021277

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG5434	Pgu1	1.17e-73	36	411	77	449	Polygalacturonase [Carbohydrate transport and metabolism].
COG3250	LacZ	1.31e-21	451	841	13	406	Beta-galactosidase/beta-glucuronidase [Carbohydrate transport and metabolism].
PRK10340	ebgA	1.70e-18	505	861	113	471	cryptic beta-D-galactosidase subunit alpha; Reviewed
PLN03003	PLN03003	6.12e-15	44	304	26	268	Probable polygalacturonase At3g15720
pfam00295	Glyco_hydro_28	1.26e-13	68	385	1	268	Glycosyl hydrolases family 28. Glycosyl hydrolase family 28 includes polygalacturonase EC:3.2.1.15 as well as rhamnogalacturonase A(RGase A), EC:3.2.1.-. These enzymes are important in cell wall metabolism.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
ADY53936.1	0.0	1	1271	4	1275
QUT89399.1	2.00e-156	23	445	28	450
ALJ59565.1	2.81e-156	23	445	28	450
QUT26779.1	6.33e-154	25	441	40	456
SDT40105.1	8.29e-132	5	454	8	464

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
3JUR_A	2.40e-89	21	444	7	438	Thecrystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_B The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_C The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima],3JUR_D The crystal structure of a hyperthermoactive Exopolygalacturonase from Thermotoga maritima [Thermotoga maritima]
5OLP_A	9.43e-81	32	436	35	447	Galacturonidase[Bacteroides thetaiotaomicron VPI-5482],5OLP_B Galacturonidase [Bacteroides thetaiotaomicron VPI-5482]
1YQ2_A	1.37e-14	505	867	122	472	ChainA, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_B Chain B, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_C Chain C, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_D Chain D, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_E Chain E, beta-galactosidase [Arthrobacter sp. C2-2],1YQ2_F Chain F, beta-galactosidase [Arthrobacter sp. C2-2]
1BHE_A	1.26e-11	33	417	4	363	ChainA, POLYGALACTURONASE [Pectobacterium carotovorum]
7VQM_A	1.04e-10	470	850	73	421	ChainA, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_B Chain B, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_C Chain C, GH2 beta-galacturonate AqGalA [Aquimarina sp.],7VQM_D Chain D, GH2 beta-galacturonate AqGalA [Aquimarina sp.]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A7PZL3	1.26e-38	44	505	65	490	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1
P20041	1.87e-15	52	384	64	417	Polygalacturonase OS=Ralstonia solanacearum OX=305 GN=pglA PE=1 SV=1
P58598	5.76e-15	61	384	78	419	Polygalacturonase OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=pglA PE=3 SV=1
Q9LW07	2.22e-14	44	381	26	328	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1
P27644	4.73e-14	198	404	25	249	Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.055328	0.899454	0.042019	0.002359	0.000430	0.000371

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001420_00339.