CAZyme Information: MGYG000001420_03019

Basic Information

• Species: Alistipes senegalensis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Rikenellaceae; Alistipes; Alistipes senegalensis
• CAZyme ID: MGYG000001420_03019
• CAZy Family: GH97
• CAZyme Description: Glucan 1,4-alpha-glucosidase SusB

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
671	MGYG000001420_2|CGC18	75663.03	4.7471

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001420	3985129	Isolate	not provided	not provided

• Gene Location: Start: 1569492; End: 1571507 Strand: +

Full Sequence      

MNQTLKFSFRNLFLSCVCLLLFACGGNPEIISPDGRTRLSFVTGADGCMAYTVERDGRPL
ILPSALGLVAQERDLAGGFSVREIVKRSVNETWTQPWGENKILRDCHNEMTAVLKNDDGV
LLTLRFRAFDDGVAFRYEWEVPDLDSLTVTDELTEFRFAEDGVSWSIPGNFNTYELLYRE
LPVSAVENANTPFTFRVDGTYGSIHEAALYDFPEMNLYRTDSLAFKAELAPLPDGIKARI
PSKFMTAWRTIQVGDKAVDLINSSLILNLNEPSKIADTSWIKPQKYIGVWWGLHLGTHTW
TMGPRHGATTENALRHIDFAAANNIQGVLFEGWNEGWENWGKTQHFDYVKPYADFDLDRI
AAYAREKNIELWMHNETGGNIPEYEAALETAMQRYAGLGVHAVKTGYAGGFRDGQLHHSQ
YGVRHYQRVVETAARYGIVIDAHEPIKDTGIRRTWPNMMTREGARGMEWNAWSEGNSAEY
LCTLPFVRLLSGPMDYTPGVFDLDYSRVRGRETGMQEWNGDNNSCCIKTTLARQIANWVI
IYSPLQMASDLIENYEGHPAFQFFRDFDADCDWSEALQGEPGEYIVVVRRADDSFFLGAG
TNDEPRTLTQKLGFLKSGMTYTATIYADAPDSAENPENYRIEKRTVTSADMLEIAMTARG
GQAVTFVPVNE

Enzyme Prediction     

No EC number prediction in MGYG000001420_03019.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH97	17	666	2e-220	0.993660855784469

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
pfam10566	Glyco_hydro_97	1.76e-136	277	567	1	277	Glycoside hydrolase 97. This domain is the catalytic region of the bacterial glycosyl-hydrolase family 97. This central part of the GH97 family protein sequences represents a typical and complete (beta/alpha)8-barrel or catalytic TIM-barrel type domain. The N- and C-terminal parts of the sequences, mainly consisting of beta-strands, form two additional non-catalytic domains. In all known glycosidases with the (beta-alpha)8-barrel fold, the amino acid residues at the active site are located on the C-termini of the beta-strands.
pfam14508	GH97_N	3.76e-84	32	272	1	235	Glycosyl-hydrolase 97 N-terminal. This N-terminal domain of glycosyl-hydrolase-97 contributes part of the active site pocket. It is also important for contact with the catalytic and C-terminal domains of the whole.
pfam14509	GH97_C	2.24e-35	571	667	1	97	Glycosyl-hydrolase 97 C-terminal, oligomerization. Glycosyl-hydrolase-97 is made up of three tightly linked and highly conserved globular domains. The C-terminal domain is found to be necessary for oligomerization of the whole molecule in order to create the active-site pocket and the Ca++-binding site.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AFL77433.1	0.0	1	671	1	671
CBK63835.1	0.0	1	671	1	671
BBL01467.1	0.0	13	671	10	669
ASB37496.1	0.0	10	670	2	664
QQR08228.1	0.0	10	670	2	664

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5HQ4_A	1.71e-166	32	671	5	663	AGlycoside Hydrolase Family 97 enzyme from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8],5HQA_A A Glycoside Hydrolase Family 97 enzyme in complex with Acarbose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQC_A	1.71e-166	32	671	5	663	AGlycoside Hydrolase Family 97 enzyme R171K variant from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
5HQB_A	4.84e-166	32	671	5	663	AGlycoside Hydrolase Family 97 enzyme (E480Q) in complex with Panose from Pseudoalteromonas sp. strain K8 [Pseudoalteromonas sp. K8]
3WFA_A	1.03e-139	29	671	4	708	Catalyticrole of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron],3WFA_B Catalytic role of the calcium ion in GH97 inverting glycoside hydrolase [Bacteroides thetaiotaomicron]
2JKA_A	1.06e-139	29	671	13	717	Nativestructure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKA_B Native structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron [Bacteroides thetaiotaomicron VPI-5482],2JKE_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKE_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with deoxynojirimycin [Bacteroides thetaiotaomicron VPI-5482],2JKP_A Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482],2JKP_B Structure of a family 97 alpha-glucosidase from Bacteroides thetaiotaomicron in complex with castanospermine [Bacteroides thetaiotaomicron VPI-5482]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
G8JZS4	1.01e-139	10	671	3	728	Glucan 1,4-alpha-glucosidase SusB OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=susB PE=1 SV=1
Q8A6L0	3.04e-53	12	664	7	659	Retaining alpha-galactosidase OS=Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50) OX=226186 GN=BT_1871 PE=1 SV=1
D7CFN7	6.58e-44	33	663	46	617	Probable retaining alpha-galactosidase OS=Streptomyces bingchenggensis (strain BCW-1) OX=749414 GN=SBI_01652 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as LIPO

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000001	0.000017	1.000014	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001420_03019.