CAZyme Information: MGYG000001436_00265

Basic Information

• Species: Paenibacillus_F sp000411255
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_F; Paenibacillus_F sp000411255
• CAZyme ID: MGYG000001436_00265
• CAZy Family: GT2
• CAZyme Description: Dimodular nonribosomal peptide synthase

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2419	MGYG000001436_1|CGC5	263981.46	4.8761

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001436	6290841	Isolate	not provided	not provided

• Gene Location: Start: 340923; End: 348182 Strand: -

Full Sequence      

MIESALRLPLSGAQQGIWYAHQMDAQRSVYNTAEWLEIHGNINEELLEQALRLTVQEAKW
LHARFGEDGDGPWQELGPETDWALLKIDVSRMNHPHAEAEAWMRQDLQKPVELAEGPLFT
QALIRLASDRWCWYQRIHHILIDAYGYALLHRRASQIYTALASGRPVPPHNFGSPEQVLR
EEETYRASGQHGQDRAYWLDKLADAPDSSVMGRGGNSPDGSPLRQSGRLAAAASRVLTES
AAEAGCSWAEAVLAAAAAYVHRMTGSADIRLGLPLMNRLGSSALNVPGMVMNVLPLCLTV
KPETSWGDLRLQVAAELRKLRRHQRYRQHDLVRDLGLLGERRRLYGPMINIIPFVETPSF
ASAGTVRHSIYAGPADDLSLHIYEASDGDGLQIDFDANPGIYSEQELSGHRRRFLSFLES
AAFADPDTPAGLLDTLLPSEKDLLLNRWIDTACDVPGDSPAELIRRRAELSPHAAAVLCG
GRTLTYAELDRQAAVLGAELRRLGIGPERLVAIALPRSEAAVVSILAVLRLGAAYMPVDP
DYPAERIAYMLDDAQPACLLTDTESMRSLPDTGNVSLLLLDELGKGDRAESPGSTPKTGD
HADAECHPLRPSPLHPAYVIYTSGSTGRPKGVPVTYGGLANLLDAMQRKFRLQEGDSFLS
VTTIAFDISVMEILLPLIGGACLDIASRELILEPGALARRIRSTGASIMQATPTLWQSLV
SARPGRLDGLRVIAGGEALPLSLQLALQELGCEVHNQYGPTETAIYSTAAALPGGSMIKP
PIGPPIANTRTYILDAGLQPVPPGAEGELYIAGAGVARGYLNKPVQTAERFVADPWGEPG
SRMYRTGDLARWLPDGSIDYLGRADHQVKLRGFRIELGEIESRIAGQPGIAQAVVVVRED
RPGERRLTAYVVPEELHEPDHAQLKNQLQQELPEYMVPLVWVTLASLPLTPNKKVDRKAL
PVPQMSGTAKEASARTPHEELLCQVFAEVLGLSSVGIDDDFFRLGGHSLLAGRAVLRIRE
TTGVDVRLSGIFEAPTVRELAQLLGRKGTPIPPVVPAAPGVAVPLSRAQRRLWFLYRLEG
PSFTYNIPLVCTLSGKLDRQALKQALLDVTGRHEPLRTVFPETPDGMPQIRIMDREAAAD
MFVFQVVPAAGLEGDDWQESLDREVRHGFELSSEIPLKAVLFEREEEEYVLLLIVHHIAA
DGWSLSVITSDLSAAYAARIAGRDPSFSDTPVRYGDFTLWQEKLLEEEGEPQSLMGSQLA
YWKEALRGLPEELPLPADHPRPSVSGQAGGVIPFRIGEELHAQLLELAAEGKASLFMVLH
AALAALLSRLGGGEDIPVGSPAAGRPDEATGSLVGMFINTLVLRADLSGQPTFRELLARV
RYTDLSAYENQELPFERLVEALSPARSRSRHPLFQIMLVLQNTPATRLDLPGIVSKLELR
GTGSSKFDLTFELTEHRRSDGEPCGIEGLLEYSADLYLPNTAEAFTRRFLKLLEQAAAQP
DQPVGRISVLLEEERLALTAKLKPEAAEQTIAGGKLSASGRTLTELFEGQAALRPDAPAV
VYGTQTLSYRQLNERANRIARLLMEEGAGPEQFVALALPRSADLPAAVLGVLKTGAAYLP
LDPDYPADRLAYMLEDAGPSILVTSADAAAGLPEGSGLKVVVLGDFYCEERLRRLPDSDP
EPALRAGRHNALHPAYLIYTSGSTGRPKGVVIPHENVIRLMESTEEWFGFDETDTWTLFH
SYAFDFSVWELWGALLYGGKLVVVPFSVSRSPSEFLDLLEEQGVTVLNQTPSAFYQLIQA
DREQSERASALSLKHVIFGGEALDPGKLEEWYSRHPDHFPRLVNMYGITETTVHVSYYPL
DRRSAEAGSGSVIGVPIPDLDVYVLDDNLEPVPFGVSGEMYVSGAGLARGYWQKPELSAQ
RFVADPFGTPGSRMYRTGDLAKRFADGSLEYLGRADQQVKLRGFRIEPGEIEAVLEKHPA
VAQAAVVLREDQPGDKRLAAYIVPAQSGEVPDTGMIRLHASAALPDHMVPSGIVVLDSLP
LTVNGKLDRKALPAPVYASANGSRSPRTPQEEVLCGLFAETLGLEDVGIDDGFFELGGHS
MLAVRLMSRIREVWGKDPGIGLLFETPTVAGLAARLEEDTGDGALEMLLPLRSNGSGAPL
FCVHPAGGLSWCYAGLMKHLNPDVPIYGLQARGIAGPDRLPQTLGEMTEDYIRCIRTVQP
AGPYRLLGWSLGGNVAHAMAVQLQKEGESVEFLAMLDAYPSHYLPLRGEPDEEEALTALL
ALGGYDPDSIGDEPLTMETAIEILRGDNSALASLSVETIRQLRVTYENSVKILAAYVPEK
YEGDLLFFHSTIIPDWFDPIDPDMWIPYVGGQIERHDIECRHKDLCQPGPLSIIGRMLAG
KLSSANMKEGEGSFHGQSV

Enzyme Prediction     

No EC number prediction in MGYG000001436_00265.

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF038078	NRPS_MxcG	0.0	7	1046	4	1045	myxochelin non-ribosomal peptide synthetase MxcG.
cd17643	A_NRPS_Cytc1-like	0.0	1555	2052	1	450	similar to adenylation domain of cytotrienin synthetase CytC1. This family of the adenylation (A) domain of nonribosomal peptide synthases (NRPS) includes Streptomyces sp. cytotrienin synthetase (CytC1), a relatively promiscuous adenylation enzyme that installs the aminoacyl moieties on the phosphopantetheinyl arm of the holo carrier protein CytC2. Also included are Streptomyces sp Thr1, involved in the biosynthesis of 4-chlorothreonine, Pseudomonas aeruginosa pyoverdine synthetase D (PvdD), involved in the biosynthesis of the siderophore pyoverdine and Pseudomonas syringae syringopeptin synthetase, where syringpeptin is a necrosis-inducing phytotoxin that functions as a virulence determinant in the plant-pathogen interaction. The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
cd05930	A_NRPS	0.0	472	960	1	444	The adenylation domain of nonribosomal peptide synthetases (NRPS). The adenylation (A) domain of NRPS recognizes a specific amino acid or hydroxy acid and activates it as an (amino) acyl adenylate by hydrolysis of ATP. The activated acyl moiety then forms a thioester bond to the enzyme-bound cofactor phosphopantetheine of a peptidyl carrier protein domain. NRPSs are large multifunctional enzymes which synthesize many therapeutically useful peptides in bacteria and fungi via a template-directed, nucleic acid independent nonribosomal mechanism. These natural products include antibiotics, immunosuppressants, plant and animal toxins, and enzyme inhibitors. NRPS has a distinct modular structure in which each module is responsible for the recognition, activation, and in some cases, modification of a single amino acid residue of the final peptide product. The modules can be subdivided into domains that catalyze specific biochemical reactions.
PRK12467	PRK12467	0.0	1064	2281	2648	3812	peptide synthase; Provisional
PRK12316	PRK12316	0.0	5	1308	2600	3869	peptide synthase; Provisional

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QND46664.1	0.0	10	2279	563	2805
ACX49739.1	7.31e-289	3	1792	5	1816
BAZ00088.1	6.11e-222	975	2406	2140	3565
BAZ75991.1	6.11e-222	975	2406	2140	3565
BAY30132.1	1.64e-220	975	2406	2142	3567

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5U89_A	0.0	437	1520	3	1072	Crystalstructure of a cross-module fragment from the dimodular NRPS DhbF [Geobacillus sp. Y4.1MC1]
6MFZ_A	4.59e-252	462	2133	209	1794	Crystalstructure of dimodular LgrA in a condensation state [Brevibacillus parabrevis],6MFZ_B Crystal structure of dimodular LgrA in a condensation state [Brevibacillus parabrevis]
6MFY_A	2.47e-238	462	2055	209	1715	Crystalstructure of a 5-domain construct of LgrA in the substrate donation state [Brevibacillus parabrevis],6MG0_A Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis],6MG0_B Crystal structure of a 5-domain construct of LgrA in the thiolation state [Brevibacillus parabrevis]
6P1J_A	1.59e-191	1064	2052	7	964	Thestructure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae],6P1J_B The structure of condensation and adenylation domains of teixobactin-producing nonribosomal peptide synthetase Txo2 serine module [Eleftheria terrae]
6N8E_A	2.20e-173	8	1044	30	1066	Crystalstructure of holo-ObiF1, a five domain nonribosomal peptide synthetase from Burkholderia diffusa [Burkholderia diffusa]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
P39846	0.0	9	2139	12	2079	Plipastatin synthase subunit B OS=Bacillus subtilis (strain 168) OX=224308 GN=ppsB PE=1 SV=1
P27206	0.0	9	2133	7	2078	Surfactin synthase subunit 1 OS=Bacillus subtilis (strain 168) OX=224308 GN=srfAA PE=1 SV=4
Q70LM5	0.0	9	2133	5188	7267	Linear gramicidin synthase subunit C OS=Brevibacillus parabrevis OX=54914 GN=lgrC PE=3 SV=1
Q70LM6	0.0	41	2153	2617	4695	Linear gramicidin synthase subunit B OS=Brevibacillus parabrevis OX=54914 GN=lgrB PE=1 SV=1
P45745	0.0	6	2406	7	2377	Dimodular nonribosomal peptide synthase OS=Bacillus subtilis (strain 168) OX=224308 GN=dhbF PE=1 SV=4

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
1.000043	0.000000	0.000000	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001436_00265.