CAZyme Information: MGYG000001437_00443

Basic Information

• Species: Staphylococcus schleiferi
• Lineage: Bacteria; Firmicutes; Bacilli; Staphylococcales; Staphylococcaceae; Staphylococcus; Staphylococcus schleiferi
• CAZyme ID: MGYG000001437_00443
• CAZy Family: GH73
• CAZyme Description: Bifunctional autolysin

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1323	MGYG000001437_1|CGC6	145108.37	9.8325

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001437	2461191	Isolate	not provided	not provided

• Gene Location: Start: 459093; End: 463064 Strand: -

Full Sequence      

MVKKLGYKTPTIIALTLAGSALTAHEAHAAEQTKAQETPTNILDEHQQVQQANQAKNETK
APTISGTQAYKDPNQVEIEKPTQPKADSSLLEQTSASSSATQQATEQEATSQNNHAQADV
AEQPVASPYAAEEIDPHHEAEVSTEAYHAAQDQKTDAQQETPQNVTKSQQSPSEALPKDD
SQNIAVQQQNNQVTTEQPTTHTQQPTHNTQPTAKKVQSKATAISAQERAAATSNQEKNLA
RVVARSAEKTPAVKQQSTSTNEKVDINANPNGPTPPRVGGKGGPATVKLEPTNYSARAVA
ATPKAYQPQVKSQINDYIRKQKFKVPKYEEDYSSFIPRYGYRYGVGKPEGIVVHDTANDN
STITSEIQYMKNNYQSAFVHGFIDGNRIIETQPTDYLAWGAGPIANQRFIHIELVHVHDY
NSFARQMNNFADYAATNLQYYGLKPDSAEYDGKGTVWTHDAVSRYLGGTDHVDPHGYLRA
HGYSYDELYDLINEKYQVKMGYASPAGSTAGQSSKPKPQNNNLKVTDNTGYSRISSKNNG
LYKTVYDQSGVRTNQTNVTLKVTKKATLNGQSFYLVSTAKNNGLLGWVRSNDVTYQSAQP
EKNVKQSYQVKPGTTVYEVPWGTKAQATGTISGSKKQTFKATKEQKVANTNWLYGTVNNL
TGWIDASNVVKTPSSSTTAPSSPLKVTGDSGFGRINNKNNGLYKTVYDAKGQPTSATNQT
LSVQKKATLNGKSFYLVSDYATGTYVGWVQQKDVDYRTGQAAKNVKQNYTIKPGATLYKV
PWGTSAQVAGKVAGKNAQNFAATKSQKVGNLTFVYGTVNQLSGWIDQSLLTQSQEKATKN
AVQSVSQIGQLKSSTEGIRASIYDKTAKNAAQFTDRTYKIAKTASNQNHNYVLLQNADGR
TPLGWFNANDVILRSLGTEKPMHGQYKVNNKTAGLYSIPWGTAKQRIDALSGNVKDRTFS
AVKSVVAGPDTYLFGQVNHKMGWINLKDLKPVASAYTVKPTSATKATKLSQPQDYFVYNG
NGYYYAKPNAQVLGSLSPYYETLFKVTSTQNVKGVTWLYGTFENGTQGWIKSGDLRSLLI
KYYNANASLKEAVDKQMALPYKPQVQHVPGKWEDANRQEVQAAMDTQNIKNSASGIYQFL
KLDQYQGLSADALNRLLAGKGILAGQGAAFAEAAKANNINEIYLISHAFLETGNGTSTLA
NGGYVDKNNKVITNGPKKFYNMFGIGAVDSDAIRGGFKTAEKYGWNTVKKAIVGGAKFIG
QNYIHAGQNTLYRMRWNPQNPATHQYATDIAWADFNAKRMKQFYDQIGEVGKYFDIDLYR
QSK

Enzyme Prediction     

EC	3.2.1.-

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH73	1168	1302	3.2e-21	0.953125

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4193	LytD	1.60e-77	1084	1320	22	245	Beta- N-acetylglucosaminidase [Carbohydrate transport and metabolism].
COG5632	CwlA	1.82e-51	314	634	5	302	N-acetylmuramoyl-L-alanine amidase CwlA [Cell wall/membrane/envelope biogenesis].
smart00047	LYZ2	1.60e-33	1156	1315	2	147	Lysozyme subfamily 2. Eubacterial enzymes distantly related to eukaryotic lysozymes.
smart00644	Ami_2	1.01e-24	346	474	1	126	Ami_2 domain.
cd06583	PGRP	4.17e-22	347	477	1	126	Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors that bind, and in certain cases, hydrolyze peptidoglycans (PGNs) of bacterial cell walls. PGRPs have been divided into three classes: short PGRPs (PGRP-S), that are small (20 kDa) extracellular proteins; intermediate PGRPs (PGRP-I) that are 40-45 kDa and are predicted to be transmembrane proteins; and long PGRPs (PGRP-L), up to 90 kDa, which may be either intracellular or transmembrane. Several structures of PGRPs are known in insects and mammals, some bound with substrates like Muramyl Tripeptide (MTP) or Tracheal Cytotoxin (TCT). The substrate binding site is conserved in PGRP-LCx, PGRP-LE, and PGRP-Ialpha proteins. This family includes Zn-dependent N-Acetylmuramoyl-L-alanine Amidase, EC:3.5.1.28. This enzyme cleaves the amide bond between N-acetylmuramoyl and L-amino acids, preferentially D-lactyl-L-Ala, in bacterial cell walls. The structure for the bacteriophage T7 lysozyme shows that two of the conserved histidines and a cysteine are zinc binding residues. Site-directed mutagenesis of T7 lysozyme indicates that two conserved residues, a Tyr and a Lys, are important for amidase activity.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QGS47148.1	0.0	1	1323	1	1323
QPA33964.1	0.0	1	1323	1	1323
QPA23734.1	0.0	1	1323	1	1323
BAS46496.1	0.0	1	1323	1	1323
AKS71798.1	0.0	1	1323	1	1323

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
4KNK_A	6.11e-107	297	508	8	223	ChainA, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNK_B Chain B, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_A Chain A, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_B Chain B, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_C Chain C, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325],4KNL_D Chain D, Bifunctional autolysin [Staphylococcus aureus subsp. aureus NCTC 8325]
3LAT_A	4.46e-99	295	507	2	213	Crystalstructure of Staphylococcus peptidoglycan hydrolase AmiE [Staphylococcus epidermidis],3LAT_B Crystal structure of Staphylococcus peptidoglycan hydrolase AmiE [Staphylococcus epidermidis]
6FXO_A	9.64e-88	1079	1320	4	244	ChainA, Bifunctional autolysin [Staphylococcus aureus subsp. aureus Mu50]
4EPC_A	8.42e-85	504	831	2	333	Crystalstructure of Autolysin repeat domains from Staphylococcus epidermidis [Staphylococcus epidermidis]
6U0O_B	7.70e-52	1080	1322	43	278	ChainB, LYZ2 domain-containing protein [Staphylococcus aureus subsp. aureus NCTC 8325]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q8CPQ1	0.0	1	1320	1	1335	Bifunctional autolysin OS=Staphylococcus epidermidis (strain ATCC 12228 / FDA PCI 1200) OX=176280 GN=atl PE=3 SV=1
Q5HQB9	0.0	1	1320	1	1335	Bifunctional autolysin OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=atl PE=3 SV=1
Q6GAG0	0.0	1	1320	1	1250	Bifunctional autolysin OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=atl PE=3 SV=1
Q6GI31	0.0	1	1320	1	1257	Bifunctional autolysin OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=atl PE=3 SV=1
Q931U5	0.0	1	1320	1	1248	Bifunctional autolysin OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=atl PE=1 SV=2

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000293	0.998952	0.000250	0.000191	0.000160	0.000140

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001437_00443.