CAZyme Information: MGYG000001447_00615

Basic Information

• Species: Prevotella oralis
• Lineage: Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae; Prevotella; Prevotella oralis
• CAZyme ID: MGYG000001447_00615
• CAZy Family: GH85
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
1245	MGYG000001447_1|CGC9	139423.93	6.2306

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001447	2849145	Isolate	not provided	not provided

• Gene Location: Start: 719273; End: 723010 Strand: -

Full Sequence      

MKKRTLPIVMLGCFSLLSVDVAAQELQESYIKWSNSSSYKLPAAISTWKNKKQITEDDNF
FISRVKPKKRFRNEATQVRLNLKEGDDKRLIAWLPFNDPNKNALPDGVFDSEVFSMWQYV
NVWGDWTAPLGRIPAALLDVAHKNGVSVSSVASIPNAYLNGDWYNYLRDLKNTNVQDAAD
YLYYYGQDGLGYNSEYWEYSNITKDVRDFHIKVTDIMRTRNSIYENIWYDGTDDNGHIRF
DQGLGSHNEKNFGTAEHPCYSLFFNYNWTNSGLLERSVKKAKEMQRDPLYLYAGINMQGG
EPKFQNWTHLKNYPISIGLWGAHQKNMFWESRNEKGSNPETQQRTYMLRTERWFTGGTRN
PANTPEVINTLNYSADNYNFPGMSSFMTAKSTLSWNLQEEPFITYFNLGNGKFFNWKGER
KNDRPWYNVGVQDYLPTWRWWFAHELLGRTNIPMGELDAEFIWNDAYVGGSCMRVYGTTS
DEYLHLFKTSYELKQGDVITVRYKLVGGKAKANLVLTTNGNESQAVNEAHYALVTTEQNA
DETEWTERKFTVNADLAGKSLALIALHFTDTKDLNLYLGEISIVRGSAATPVKPSIKNAK
LLAFGKLGVDGKIIFNMQNSKSEGEPCYNSDVNTSLFKLYAKQGDGEPILMGITTSWAGM
YYSIPVDFTKNSFNLRLGVSAVSLDMKTESEIVWSDELTPPQYVYDDGIQIDKNVIKPNE
SFNLSYVDPRHTTGTWTIYDADHNKVYSGTGTVVDVTGLPKTGVYSLELNGEVHDGATTS
TQTRTFAGYIQITPEAIGALPQINSLTANGQEQTINVNTGDMIQMAYTGRHANGQLSRGI
ETKEKGIAFKATDAGLTNNKSAWSLAFWVKYNSIVDGSNQFVDMRDQFTSWPQNNWGCFW
STYDGKGKTLQFTIRNANSGGDEHKQNWQVDFTPGVWTHVVIAMESNGTGVRERIYINGK
QATPTNWELGSIKGTGLSTVYQNTTAWWPNAYMLLGIGRHGCAALNGVVDDVKFFDRTLT
ESEVEKVMTTSDLSDAPKASWNFETDATADNYFISNGQDTSVKLARVEIKAGENEGQGTL
ANTVPDYQAGCPFVSGTAYTVTTVPVWKAPKGALTETSGSDMQGQSKVSYTSAGDYAVTL
TLTNSYGSDSRTFQVIKVTSATGIHQAENAEIRTYTVDRDIFVEFADAGKYGVQVYTAQG
LQVANAAHDVVTGTKVHVHVTVAGVYLLRIVKDGKTVRTVKLICK

Enzyme Prediction     

No EC number prediction in MGYG000001447_00615.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH85	108	414	9.9e-52	0.946031746031746

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
COG4724	COG4724	9.19e-39	37	583	31	549	Endo-beta-N-acetylglucosaminidase D [Carbohydrate transport and metabolism].
cd06547	GH85_ENGase	8.13e-29	108	441	21	334	Endo-beta-N-acetylglucosaminidase (ENGase) hydrolyzes the N-N'-diacetylchitobiosyl core of N-glycosylproteins. The beta-1,4-glycosyl bond located between two N-acetylglucosamine residues is hydrolyzed such that N-acetylglucosamine 1 remains with the protein and N-acetylglucosamine 2 forms the reducing end of the released glycan. ENGase is a key enzyme in the processing of free oligosaccharides in the cytosol of eukaryotes. Oligosaccharides formed in the lumen of the endoplasmic reticulum are transported into the cytosol where they are catabolized by cytosolic ENGases and other enzymes, possibly to maximize the reutilization of the component sugars. ENGases have an eight-stranded alpha/beta barrel topology and are classified as a family 85 glycosyl hydrolase (GH85) domain. The GH85 ENGases are sequence-similar to the family 18 glycosyl hydrolases, also known as GH18 chitinases. An ENGase-like protein is also found in bacteria and is included in this alignment model.
pfam03644	Glyco_hydro_85	7.48e-24	109	411	5	291	Glycosyl hydrolase family 85. Family of endo-beta-N-acetylglucosaminidases. These enzymes work on a broad spectrum of substrates.
pfam13385	Laminin_G_3	1.64e-13	850	1024	8	150	Concanavalin A-like lectin/glucanases superfamily. This domain belongs to the Concanavalin A-like lectin/glucanases superfamily.
smart00089	PKD	0.002	1128	1156	49	77	Repeats in polycystic kidney disease 1 (PKD1) and other proteins. Polycystic kidney disease 1 protein contains 14 repeats, present elsewhere such as in microbial collagenases.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EFC71164.1	0.0	1	1245	1	1247
QUB60779.1	0.0	4	1245	2	1250
QUB72358.1	0.0	4	1245	2	1250
QUB76735.1	0.0	4	1245	2	1250
ADK97201.1	0.0	4	1245	2	1245

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
2VTF_A	6.28e-32	79	683	49	608	X-raycrystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae],2VTF_B X-ray crystal structure of the Endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae E173Q mutant reveals a TIM barrel catalytic domain and two ancillary domains [Glutamicibacter protophormiae]
3FHA_A	4.46e-31	79	683	44	603	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_C Chain C, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHA_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]
3FHQ_A	4.46e-31	79	683	44	603	ChainA, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_B Chain B, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_D Chain D, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae],3FHQ_F Chain F, Endo-beta-N-acetylglucosaminidase [Glutamicibacter protophormiae]

Swiss-Prot Hits     

has no Swissprot hit.

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000620	0.993860	0.004799	0.000248	0.000236	0.000220

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001447_00615.