CAZyme Information: MGYG000001451_03322

Basic Information

• Species: Paenibacillus_A antibioticophila
• Lineage: Bacteria; Firmicutes; Bacilli; Paenibacillales; Paenibacillaceae; Paenibacillus_A; Paenibacillus_A antibioticophila
• CAZyme ID: MGYG000001451_03322
• CAZy Family: PL8
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2139	MGYG000001451_2|CGC9	232907.7	4.6981

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001451	5573040	Isolate	not provided	not provided

• Gene Location: Start: 526394; End: 532813 Strand: +

Full Sequence      

MSKLLKSLMAFVLLVGTWGGMPANVVRAADRSTLSMDQTVVELGQPLTIHYTGAQGNKDW
IGIYLKDETPKKGTNAWKWDYVNDLPDGTVTLTDNSEGRKEYPSLKDLAPGEYYIAYLLN
DGYTENADRFYFQIADKEVTQLKATPESVALKLNPQEDSSQLQVEATYSTNETEDVTSAN
DIQYVSSLPQVATVTSTGLVKAVSAGTAVIDITFKGKSATVEVTVASTTPGPDPKPEIEL
TVMSLNLWAGDAIKKNGKDSVVNFLENLIRTADADVIGIQESNDDVVTQAAASLGYDFSM
KASPNVSLMSRYPILDVDKEKDYYLIEVDPGRAVAISNVHLSSSPYGPYSIADGKTVESV
LADENLKHMAEMRNSFSKLPALAGQGMPVFLTGDFNVPSHLDWTEATKDRYFGKAVEWPV
SKKLQELGMVDSYRSMHPDPASTPGITWAVEGTEWRTPEVYDRIDYVYAGGPATTLDSKL
IGEKTVNGGPISYADIQLDPYLSDHRAVVSKFNVMPRSYADLNLPSAPKPDYSSTLSMDQ
TVVERGQPLTIHYTGAQGQKDWIGIYLKNETPKKGTNAWKWAYVNDLPDGTVTLTDNSEG
RKEYPSLKDLAPGEYYLAYLLNDGYTENADRFYFQIVDKVSTPEEIADMKLMKQRIVDYY
ISRDIINDGTNGRVEWTFKSKAGTYLSSQNPDGSWGDVDYKSTTSAANGRGWSPYLALDR
MQSMAQAFADPNDPYYHSPAMLEGIQKALDHWFTVKPTSTNWWETGIGKQLRLGKIALLC
EGYLTETQASNIIATLDSKAHTGDGANSSWYNQNYMYRGLLLENADIVRDAVNAFNILAN
VTTTVTGIQSDMSFFMHGKTNYTTGYGRSFARDMSFWAYITSGTVFPYSKVAIDSLSSYI
LDGTRYLVRGDVADLGMGMNGPEWPSYSSAALTFYEDPLEWMITANPARAAEFASFLDNI
RRVGTSTSNGLNANNMTQWQTLVSSHMRDDYGITVKMSSSTVKGGEWRTINPSGYNLLYW
TPQGATAIQRTGDEYRPVYQLMDWAHVPGTTAPYVLTKDGNFNNPRTFVGGVTDERYGAT
AFDFNKLSTSGKKGYFFFDDEMVALGAGITSTNAAPVHTTLNQSQAVGEVLVDGNPLAEG
TKQTNGRWAYNDRIGYVFPNSTAFQVNLETKTGSWSDVVTGSSTDPITKPIFSVWLDHGI
KPANASYQYIVLPNKNSDEVSNYASNIPVTILSNTSSVQAVRHNTLEISELLFYKPGTVT
LRDGLTVTVDQPAMVIVDESAAPVRISVSNPETPGISVNVTLDRNGERTTTSYKLGKDTF
TGRSITLDEKASINDREFDLAYDKGAIASSSKGNHASSNATDIYRTSYWNSANSDNEWIY
VDLQSQYLINKVRLNWEKAYGKSYKIQVSNDANTWTDVYSTTAGKGGIEDISFNPVSARY
VRMQGVQQGTGNGYSLYEFKVYEDLPLNLAEGKSVVASSSRAADVAPGYAVDGILSTRWG
SNYSDSEWIYVDLGSSQSIEKVDLHWEAAYGKEYQIRVSDDAKNWTTVYSTTTGAGGIDK
ISFAPVNARYVQMYGIKRGSTYGYSLWEFKVYPSLVAPPEAPADVYAIGHDGSAEVSFTA
PIHHGGSEITGYQVTAWADGKAVTTAEGSDSPITVTGLTNGTAYTFTVIARNAKWESVPS
APSNSVIPSPNEASVPAAPANLKATAGDQSVTLRWNASAESVTYSVYQYEGIAAPANPDN
WQLVQASVTEATYTVTGLTNGKGYTFAVKAVDARGESDFSNTVTATPMVRQPETPNNPYP
GTQAGGSTTGNGNHTSIIKSANGTIIIPNGRAGEVSLGEEVILTIGAGAAEQELRIVIEK
LLSTAGLVFDKETLVSNVFELTKNVTGNFKKPIVLSLKFDPSLVGDHQRAAIFYYDEERK
VWIEIGGIVNGDRITAEVDHFTKFAVMAVGEKKDEGGEPDTPTPSFTDIAGHWAQNAILC
AAGIKLVNGYPDGTFKPDADITRAEFTVMLAKALKLEAAGSAAAFTDEAKIGGWAKQAIA
NAVEAGLVSGYADGSFRPDARITRAEMAAMIARALKLSAEESATTGFADDKDIPQWAKSA
IEAVRRLGIVSGRGDNKFVPSGTATRAEAVTLLVRTLEQ

Enzyme Prediction     

No EC number prediction in MGYG000001451_03322.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
PL8	979	1214	2.2e-60	0.9759036144578314
CBM32	1476	1590	3.8e-26	0.9274193548387096
CBM32	1348	1460	8.1e-25	0.9193548387096774

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd01083	GAG_Lyase	6.55e-117	652	1291	5	693	Glycosaminoglycan (GAG) polysaccharide lyase family. This family consists of a group of secreted bacterial lyase enzymes capable of acting on glycosaminoglycans, such as hyaluronan and chondroitin, in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen. These are broad-specificity glycosaminoglycan lyases which recognize uronyl residues in polysaccharides and cleave their glycosidic bonds via a beta-elimination reaction to form a double bond between C-4 and C-5 of the non-reducing terminal uronyl residues of released products. Substrates include chondroitin, chondroitin 4-sulfate, chondroitin 6-sulfate, and hyaluronic acid. Family members include chondroitin AC lyase, chondroitin abc lyase, xanthan lyase, and hyalurate lyase.
pfam02278	Lyase_8	6.08e-71	986	1216	12	252	Polysaccharide lyase family 8, super-sandwich domain. This family consists of a group of secreted bacterial lyase enzymes EC:4.2.2.1 capable of acting on hyaluronan and chondroitin in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen.
NF033190	inl_like_NEAT_1	1.29e-21	1941	2139	559	754	NEAT domain-containing leucine-rich repeat protein. Members of this family have an N-terminal NEAT (near transporter) domain often associated with iron transport, followed by a leucine-rich repeat region with significant sequence similarity to the internalins of Listeria monocytogenes. However, since Bacillus cereus (from which this protein was described, in PMID:16978259) is not considered an intracellular pathogen, and the function may be iron transport rather than internalization, applying the name "internalin" to this family probably would be misleading.
pfam00754	F5_F8_type_C	2.35e-21	1474	1588	1	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.
pfam00754	F5_F8_type_C	6.03e-21	1346	1458	2	125	F5/8 type C domain. This domain is also known as the discoidin (DS) domain family.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
QMV41393.1	0.0	1	2138	1	2235
CQR51520.1	0.0	644	2138	43	1610
QQZ62770.1	0.0	649	2138	1	1563
QUL55219.1	0.0	644	2138	19	1488
AKH86410.1	1.06e-253	647	1592	38	972

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
1CB8_A	1.88e-66	686	1277	32	625	CHONDROITINASEAC LYASE FROM FLAVOBACTERIUM HEPARINUM [Pedobacter heparinus]
1HM2_A	2.88e-66	686	1277	54	647	ACTIVESITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HM3_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-Oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus],1HMU_A ACTIVE SITE OF CHONDROITINASE AC LYASE REVEALED BY THE STRUCTURE OF ENZYME-OLIGOSACCHARIDE COMPLEXES AND MUTAGENESIS [Pedobacter heparinus],1HMW_A Active Site Of Chondroitinase Ac Lyase Revealed By The Structure Of Enzyme-oligosaccharide Complexes And Mutagenesis [Pedobacter heparinus]
6F2P_A	3.16e-42	761	1334	122	738	Structureof Paenibacillus xanthan lyase to 2.6 A resolution [Paenibacillus]
2E24_A	7.40e-37	695	1291	43	697	ChainA, Xanthan lyase [Bacillus sp. GL1]
2E22_A	1.30e-36	695	1291	43	697	Crystalstructure of xanthan lyase in complex with mannose [Bacillus sp. GL1]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
Q59288	1.58e-65	686	1277	54	647	Chondroitinase-AC OS=Pedobacter heparinus (strain ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3) OX=485917 GN=cslA PE=1 SV=1
Q9AQS0	1.60e-35	695	1291	68	722	Xanthan lyase OS=Bacillus sp. (strain GL1) OX=84635 GN=xly PE=1 SV=1
P38536	2.46e-30	1876	2139	1602	1857	Amylopullulanase OS=Thermoanaerobacterium thermosulfurigenes OX=33950 GN=amyB PE=3 SV=2
Q9GUC9	2.75e-24	240	472	27	274	Uncharacterized protein F14F9.5 OS=Caenorhabditis elegans OX=6239 GN=F14F9.5 PE=1 SV=2
P38535	7.49e-23	1965	2139	907	1083	Exoglucanase XynX OS=Acetivibrio thermocellus OX=1515 GN=xynX PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.000369	0.998703	0.000268	0.000218	0.000208	0.000194

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001451_03322.