CAZyme Information: MGYG000001459_01822

Basic Information

• Species: Fusobacterium nucleatum
• Lineage: Bacteria; Fusobacteriota; Fusobacteriia; Fusobacteriales; Fusobacteriaceae; Fusobacterium; Fusobacterium nucleatum
• CAZyme ID: MGYG000001459_01822
• CAZy Family: GH23
• CAZyme Description: Membrane-bound lytic murein transglycosylase F

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
190	MGYG000001459_40|CGC1	21682.17	5.4659

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001459	2247318	Isolate	not provided	not provided

• Gene Location: Start: 14768; End: 15340 Strand: -

Full Sequence      

MKKNILLLLLLLFSSKPIFSKENFVNKVKEYVISNVDNYELATELYGYIKYYAKEYGVEE
ELVVSVIKVESNYDVVAVSPVGAIGLMQLMPKTAEYLGVEAEYIPENIKGGVKYLKECLS
KNGEDIALALAAYNAGLGAVKKYSSIPPYRETQKYIEKVLKEYNKLKKEKYVHNIVEFKE
DSKIAFEDEF

Enzyme Prediction     

No EC number prediction in MGYG000001459_01822.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GH23	60	172	1.2e-27	0.8444444444444444

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
cd00254	LT-like	5.73e-46	60	164	1	111	lytic transglycosylase(LT)-like domain. Members include the soluble and insoluble membrane-bound LTs in bacteria and LTs in bacteriophage lambda. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd16896	LT_Slt70-like	1.20e-37	48	165	7	146	uncharacterized lytic transglycosylase subfamily with similarity to Slt70. Uncharacterized lytic transglycosylase (LT) with a conserved sequence pattern suggesting similarity to the Slt70, a 70kda soluble lytic transglycosylase which also has an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.
cd13401	Slt70-like	7.67e-35	48	160	9	143	70kDa soluble lytic transglycosylase (Slt70) and similar proteins. Catalytic domain of the 70kda soluble lytic transglycosylase (LT)-like proteins, which also have an N-terminal U-shaped U-domain and a linker L-domain. LTs catalyze the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc), as do "goose-type" lysozymes. However, in addition to this, they also make a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue. Proteins similar to this family include the soluble and insoluble membrane-bound LTs in bacteria and the LTs in bacteriophage lambda.
pfam01464	SLT	4.18e-27	49	155	1	114	Transglycosylase SLT domain. This family is distantly related to pfam00062. Members are found in phages, type II, type III and type IV secretion systems.
cd13403	MLTF-like	6.92e-25	49	168	1	161	membrane-bound lytic murein transglycosylase F (MLTF) and similar proteins. This subfamily includes membrane-bound lytic murein transglycosylase F (MltF, murein lyase F) that degrades murein glycan strands. It is responsible for catalyzing the release of 1,6-anhydromuropeptides from peptidoglycan. Lytic transglycosylase catalyzes the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyl-D-glucosamine (GlcNAc) as do goose-type lysozymes. However, in addition, it also makes a new glycosidic bond with the C6 hydroxyl group of the same muramic acid residue.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
EFG34987.1	1.17e-126	1	190	1	190
EEO42572.1	1.66e-126	1	190	1	190
ATV71382.1	7.84e-110	2	190	3	194
ASC04268.1	7.84e-110	2	190	3	194
ALM95353.1	7.84e-110	2	190	3	194

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
5MPQ_A	6.87e-08	52	160	423	549	BulgecinA: The key to a broad-spectrum inhibitor that targets lytic transglycosylases [Neisseria meningitidis]
5O1J_A	6.88e-08	52	160	427	553	Lytictransglycosylase in action [Neisseria meningitidis MC58]
6FPN_B	6.90e-08	52	160	433	559	Lytictransglycosylase in action [Neisseria meningitidis MC58]
5O24_A	6.91e-08	52	160	437	563	Lytictransglycosylase in action [Neisseria meningitidis]
5O29_A	6.92e-08	52	160	443	569	Lytictransglycosylase in action [Neisseria meningitidis]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
O31608	3.63e-30	49	163	64	179	Putative murein lytic transglycosylase YjbJ OS=Bacillus subtilis (strain 168) OX=224308 GN=yjbJ PE=3 SV=1
O64046	9.28e-27	47	165	1424	1543	Probable tape measure protein OS=Bacillus phage SPbeta OX=66797 GN=yomI PE=3 SV=1
O31976	9.28e-27	47	165	1424	1543	SPbeta prophage-derived uncharacterized transglycosylase YomI OS=Bacillus subtilis (strain 168) OX=224308 GN=yomI PE=3 SV=2
P27380	1.86e-10	55	166	17	122	Transglycosylase OS=Enterobacteria phage PRD1 OX=10658 GN=VII PE=1 SV=3
Q0A9W6	1.55e-09	30	138	274	392	Membrane-bound lytic murein transglycosylase F 1 OS=Alkalilimnicola ehrlichii (strain ATCC BAA-1101 / DSM 17681 / MLHE-1) OX=187272 GN=mltF1 PE=3 SV=1

SignalP and Lipop Annotations

This protein is predicted as SP

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.001428	0.996463	0.001409	0.000221	0.000221	0.000224

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001459_01822.