CAZyme Information: MGYG000001465_02299

Basic Information

• Species: Providencia alcalifaciens_A
• Lineage: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Providencia; Providencia alcalifaciens_A
• CAZyme ID: MGYG000001465_02299
• CAZy Family: GT44
• CAZyme Description: hypothetical protein

CAZyme Property

Protein Length	CGC	Molecular Weight	Isoelectric Point
2980		339134.02	7.3442

Genome Property

Genome Assembly ID	Genome Size	Genome Type	Country	Continent
MGYG000001465	4108375	Isolate	not provided	not provided

• Gene Location: Start: 17751; End: 26693 Strand: -

Full Sequence      

MRVNSVIFNSPQENNTTALNNTSNDIGISDKSEKTYEPFINRITKIKNTSTEDVVAIGGF
IQEEIRKTLNPTTSQHPVTTSGNWKTAILYRIGLLMSGHASPSTPLINGNNNVNLNDIES
VASKEPIISNLAPLEHVYNTPSLTIPHELEVKSKVIKKRSLEDGSKEQKAHHLNTNGKYS
LTPNNILDKIHNGSKKKHEIRPELISLLKQSINGYLNLKDKNSREGIEMLKQQANTLSNI
YYSLNKHGNTRNNEVEKNNIIKLIAEVQNEYKSHKVEIEKNINIAWIAGVPPESITRYAK
AYKNAYPDFIYNFWTDSHAMLASAFNKELRIIAFENAKNELLSLLSYEEIHRLKNGQDFS
ESFNIKLTDLFERCLFKSVLQVQDSVMNYAYIKGLLTFNDNHRIEFLKEILNYDAEKIKE
FQQIVEENNRKLRELEKKLIDIFGKENVNIKDVNELPEMQIAHKKQNYQQELILRGNYAA
ATDMLRAYILKNHGGIYADHDVMPNYTPEVYKIVQDNCNNYDFLEKEDHRRALSDEILSL
VSNEPSAGLKHKLPREDITKLDNIISKLKIQVKESKIFSPIDTKVIRDSMMMHKSHQLSR
KGWKIRGNNNFLATHKESKVMDFVIAGQENTYREILGIREQLRTEGIGEQRAFYNPNNDS
LEPLRGSEKVESKLFVSDLESNEKKSRKKEILKSLDKDLKEYGQLLTVENQGVKKKDIRA
TQNFLKGYKEGNAINNIFGFKEEMNIHDIVSLMKKNYKNLSEEQKGALAYEVEKRALAVT
FQPRVEEYHQLFDKVKSNGEIDPLAKEKLIPQLFLLNLVGDGFGGRCDPLSVLLLSAKYQ
ESLTNENLSGKFIENLYSAAAVLSESSRYTKTEIMKAKELLNTLVRLHSKNPMLSTDQQV
WKEKKEKQTLHSTIELLKKESIEPILLKLEAPGHAMAAWAVGKGENRIYGFYDPNGGFVE
FSDIEKFSHYFYGIFENNGLDKGKKYHLSKDTEGEKFIFERVVILNGEKLAHYKTGINEK
PLLDILKINVFEPEIRIKQKKEKFSPKKYYQGSLFSHYRMDGIIPQKYSTLSITGPDAMM
NSMKKYYESLGELGQCRLEQHNNKFKGLGDDSFMGNLKEVVGASGQHYDWINQKTVGIND
ITPDDESTWVGKKLDIKEVLTSIESNKQSSILFDITPTKLNLEKLQTGWPSELVQKLKLE
WPNFEDDYNKLIDRNILDLNMLSDIDQKIHKHLLTCEDSLVKWAGLSLSDQLTNALHRKK
TPINNQVHYLLSDLSQDIHGYKKSIRSILASNISTKISIWRDDFINKLLVAKEFTILKAR
ENEIINLFKEYNGVLGYELELYYGLKRKSQLGTISVTEKEKLSTVLQKIAQDKNLEKKLI
EIERKIKSLPIQDNFKINGKTINFLKIKDINDLKYNIGYKGDIITLWNKYITNEQQIINS
LLQEADNKWGKGKIEIRNISTDLKDNHLIQKMINDRYGFDDLNDIVKHGILRQESGVMMR
HPALAAPSTELIKIISDYTGGNDTDTKIILKKLYNHFFGKDIVHFGPSNYEQSLRLTFES
ILDVLDKNNFSKYFLSVMNQDVSALGVRYSSVEGKLTSDIMMSGMKDILSNNNIVLDRMG
NFLEVLYDVKKSKDITLELIKSKFNQNTLGFMLQNDAHILEYLKGTSTHHESSLSDISRG
LTGKNSFLECALHITQDKFPSITNNLLKEINSKQPSVYSIADSTLIEQSTLKGLGYGSND
NYLTNPIAGPKLHDISIQAKYNALKWGDFYGRNALLWQDAATKFEGKNVQFNPQILLTPQ
EGRCMGLAELYLLVKNENHYNILQENLELASALYQESLLEQSQLSLADKHLLTSMLNQIE
HAQQHGNNKLLQSTNIEKIRLSDFEVNTVAEYLSANHIKKLLITMNFHSIVISIFEDKCR
VTDPNFGSADFTNLEQALNFVESSIHISPEVRELYSGKAIGDNIDILFVRNNHWESIVIP
DVLNLSTHHYQSTVEKIKSLNFIIKIDNNQFNLFDLYKYGIYWNGARIDENINKTVFNSN
ELDKLNINEKILKKYIDEHHLTEKEHDAIKLLVETLKSSDGEKKLNLDDVFFSDNKDKHF
SHRVQRQSERVGQMLSNIYSRIRNAISSTGISQFKIKNTILSDSSETIKLALEELDTRKV
VNVDINISDLKMTLREGLDALSEGVDNMNLDGVMAILGIIQYVRLTHNGDYVSTVDHANL
GSDIKTVTEKIVGTTLMFMGNKKFGTSISDISLEGIVSQQISQLATKIGGSTGKVLSHVA
KLIRFPVLDTALNLWSLGESVQSYLDAKEGSLEKTLAEIDISFASTYSALTLSSIAFPPI
GLAAFPLMFLQQEIRNFQMHLYHENSRRASWLNIETFFNQAAESILNIDKYNGVIDLSSC
QIVGNLKLDLTSNPPILTGIPSYNDGKNIGNNPNLSDEEVRKISQYAISCVNKDEVYIPN
IFGNSGGTHCRDRSSESNLVKGFANRNWPSKMPSIPAGDYNTAILGYTATFTANTEVIRM
AWDDYQEVSRENYQAIEKSHKHTKVIAGEKNFRVIVPALERSMFSPQNLGELLQFADYSF
DINGGIGGITVYANGVGHFTIRGENGAKNTISFGELPEYLNVHLDLSKTGKQDVVTYNVA
SYPYKQKTLMTLIQDNINTVVGSHHRKNTFIGNNQGNHFIIGHSGANVYLGGGSNVITIP
SLSGKSFIANIYLTDDAQMQYLQFGFGINKIFIANRYHSEILFYFDDNTRYDEKILMIHA
PNKRFIGGFLDSLMISTADGLELGINKSGLLFAKKINVIEFSKYHKYNHILTPKEVLQNE
KFRVLKGLIYEFDYGDHKAIYEENSLIYKGLSSGCKIQLSNEFSSVLYGSKGGEYIFPGD
NGNNYTIYLHNDNENPETINLSGVSNKIKVTAYCKHSKCILKFFFNDTYSTLIIKSNSID
NQSLQKYNVNIIFNKNKEMQLSDIFKLCSHMENEVVIYNN

Enzyme Prediction     

No EC number prediction in MGYG000001465_02299.

CAZyme Signature Domains

Family	Start	End	Evalue	family coverage
GT44	416	513	2e-28	0.97

CDD Domains     

Cdd ID	Domain	E-Value	qStart	qEnd	sStart	sEnd	Domain Description
NF033479	Efa1_rel_toxin	0.0	1047	2902	693	2546	LifA/Efa1-related large cytotoxin. Members of this family are large and almost certainly multifunctional proteins found in various pathogens from genus Chlamydia, about 3000 amino acids in size and related to lymphostatin (Efa1/LifA) from enteropathogenic Escherichia coli. Roles have been suggested for Efa1 (EHEC factor for adherence) in adhesion, so some members have been annotated as adherence proteins rather than cytotoxins.
NF033479	Efa1_rel_toxin	5.98e-102	47	640	13	664	LifA/Efa1-related large cytotoxin. Members of this family are large and almost certainly multifunctional proteins found in various pathogens from genus Chlamydia, about 3000 amino acids in size and related to lymphostatin (Efa1/LifA) from enteropathogenic Escherichia coli. Roles have been suggested for Efa1 (EHEC factor for adherence) in adhesion, so some members have been annotated as adherence proteins rather than cytotoxins.
pfam12919	TcdA_TcdB	4.90e-39	280	635	1	316	TcdA/TcdB catalytic glycosyltransferase domain. This domain represents the N-terminal glycosyltransferase from a set of toxins found in some bacteria. This domain in TcdB glycosylates the host RhoA protein.
cd20495	C58_PaToxP-like	9.69e-39	805	1004	2	178	peptidase C58 domain of Photorhabdus asymbiotica toxin PaTox and LifA/Efa1-related large cytotoxin, and similar proteins. This subfamily includes the cysteine protease domain of Photorhabdus asymbiotica toxin PaTox, a large virulence-associated multifunctional protein toxin. This domain is similar to AvrPphB protease found in Pseudomonas syringae, a C58 protease. Mutation studies show that this domain enhances cytotoxic effects of the toxin, and therefore is essential for full PaTox activity. Also included in this family is the enteropathogenic Escherichia coli (EPEC) factor for adherence/lymphocyte activation inhibitor (efa1/lifA) gene which is strongly associated with diarrhea. Efa1/LifA proteins are important for A/E lesion formation efficiency in EPEC strains lacking multiple effectors. This domain contains the invariant C/H/D residues conserved in the C58/YopT family.
pfam11996	DUF3491	1.77e-27	2529	2902	7	379	Protein of unknown function (DUF3491). This family of proteins is functionally uncharacterized. This protein is found in bacteria. Proteins in this family are typically between 286 to 3225 amino acids in length. This protein is found associated with pfam04488. This protein is found associated with pfam04488.

CAZyme Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End
AVF62353.1	0.0	16	2979	3	2971
AMG08231.1	0.0	20	2979	4	2967
AMG29389.2	0.0	135	2980	12	2867
AUJ89621.1	0.0	31	2979	19	2972
QST64945.1	0.0	31	2979	19	2972

PDB Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
6II2_A	4.55e-80	690	1032	315	661	Crystalstructure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_B Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_C Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II2_D Crystal structure of alpha-beta hydrolase (ABH) and Makes Caterpillars Floppy (MCF)-Like effectors of Vibrio vulnificus MO6-24/O [Vibrio vulnificus]
6II0_A	5.45e-78	695	1032	5	346	Crystalstructure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_B Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_C Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II0_D Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O [Vibrio vulnificus],6II6_A Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3) [Vibrio vulnificus],6II6_B Crystal structure of the Makes Caterpillars Floppy (MCF)-Like effector of Vibrio vulnificus MO6-24/O in complex with a human ADP-ribosylation factor 3 (ARF3) [Vibrio vulnificus]
7S0Z_A	3.66e-14	203	512	23	299	ChainA, Toxin B [Clostridioides difficile],7S0Z_B Chain B, Toxin B [Clostridioides difficile]
6OQ7_A	3.75e-14	203	512	23	299	ChainA, Toxin B [Clostridioides difficile]
7V1N_A	4.95e-14	203	512	23	299	ChainA, Toxin B [Clostridioides difficile]

Swiss-Prot Hits     

Hit ID	E-Value	Query Start	Query End	Hit Start	Hit End	Description
A0A2S3R7M0	7.75e-76	690	1032	3215	3561	Multifunctional-autoprocessing repeats-in-toxin OS=Vibrio vulnificus OX=672 GN=CRN52_02910 PE=1 SV=1
Q9EXR0	2.71e-13	203	512	23	299	Toxin B OS=Clostridioides difficile OX=1496 GN=tcdB PE=1 SV=1
P18177	8.90e-12	180	515	2	302	Toxin B OS=Clostridioides difficile OX=1496 GN=tcdB PE=1 SV=3
Q46342	7.64e-11	203	514	23	301	Cytotoxin-L OS=Paeniclostridium sordellii OX=1505 GN=tcsL PE=1 SV=1
P0DUB4	1.31e-10	203	514	23	301	Cytotoxin-L OS=Paeniclostridium sordellii OX=1505 GN=tcsL PE=1 SV=1

SignalP and Lipop Annotations

This protein is predicted as OTHER

Other	SP_Sec_SPI	LIPO_Sec_SPII	TAT_Tat_SPI	TATLIP_Sec_SPII	PILIN_Sec_SPIII
0.999996	0.000041	0.000001	0.000000	0.000000	0.000000

TMHMM  Annotations     

There is no transmembrane helices in MGYG000001465_02299.